Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein
The glycosylation of asparagine residues in proteins is known to occur by transfer from a dolichyl diphosphate oligosaccharide containing glucose. Paper chromatography allowed the separation of oligosaccharides (obtained by acid hydrolysis of the dolichyl diphosphate derivative) containing 1, 2 and...
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todo:paper_00142956_v105_n2_p275_STANELONI2023-10-03T14:11:46Z Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein STANELONI, R.J. UGALDE, R.A. LELOIR, L.F. dolichol D glucosylmonophosphate dolichol-D-glucosylmonophosphate dolichyl diphosphate oligosaccharides glucose isoprenoid phosphate sugar protein animal article liver microsome metabolism paper chromatography rat Animal Chromatography, Paper Glucose Microsomes, Liver Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars Proteins Rats The glycosylation of asparagine residues in proteins is known to occur by transfer from a dolichyl diphosphate oligosaccharide containing glucose. Paper chromatography allowed the separation of oligosaccharides (obtained by acid hydrolysis of the dolichyl diphosphate derivative) containing 1, 2 and 3 glucose residues. Using this procedure it was found that the addition of all three glucoses to the dolichyl diphosphate oligosaccharide occur with dolichyl phosphate glucose as donor. Furthermore only the compound with three glucoses was used as donor in the transfer to protein. The addition of glucose to exogenous dolichyldiphosphate oligosaccharide labelled by transfer from radioactive guanosine diphosphate mannose was detected. Copyright © 1980, Wiley Blackwell. All rights reserved JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v105_n2_p275_STANELONI |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
dolichol D glucosylmonophosphate dolichol-D-glucosylmonophosphate dolichyl diphosphate oligosaccharides glucose isoprenoid phosphate sugar protein animal article liver microsome metabolism paper chromatography rat Animal Chromatography, Paper Glucose Microsomes, Liver Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars Proteins Rats |
spellingShingle |
dolichol D glucosylmonophosphate dolichol-D-glucosylmonophosphate dolichyl diphosphate oligosaccharides glucose isoprenoid phosphate sugar protein animal article liver microsome metabolism paper chromatography rat Animal Chromatography, Paper Glucose Microsomes, Liver Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars Proteins Rats STANELONI, R.J. UGALDE, R.A. LELOIR, L.F. Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein |
topic_facet |
dolichol D glucosylmonophosphate dolichol-D-glucosylmonophosphate dolichyl diphosphate oligosaccharides glucose isoprenoid phosphate sugar protein animal article liver microsome metabolism paper chromatography rat Animal Chromatography, Paper Glucose Microsomes, Liver Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Oligosaccharides Polyisoprenyl Phosphate Sugars Proteins Rats |
description |
The glycosylation of asparagine residues in proteins is known to occur by transfer from a dolichyl diphosphate oligosaccharide containing glucose. Paper chromatography allowed the separation of oligosaccharides (obtained by acid hydrolysis of the dolichyl diphosphate derivative) containing 1, 2 and 3 glucose residues. Using this procedure it was found that the addition of all three glucoses to the dolichyl diphosphate oligosaccharide occur with dolichyl phosphate glucose as donor. Furthermore only the compound with three glucoses was used as donor in the transfer to protein. The addition of glucose to exogenous dolichyldiphosphate oligosaccharide labelled by transfer from radioactive guanosine diphosphate mannose was detected. Copyright © 1980, Wiley Blackwell. All rights reserved |
format |
JOUR |
author |
STANELONI, R.J. UGALDE, R.A. LELOIR, L.F. |
author_facet |
STANELONI, R.J. UGALDE, R.A. LELOIR, L.F. |
author_sort |
STANELONI, R.J. |
title |
Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein |
title_short |
Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein |
title_full |
Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein |
title_fullStr |
Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein |
title_full_unstemmed |
Addition of Glucose to Dolichyl Diphosphate Oligosaccharide and Transfer to Protein |
title_sort |
addition of glucose to dolichyl diphosphate oligosaccharide and transfer to protein |
url |
http://hdl.handle.net/20.500.12110/paper_00142956_v105_n2_p275_STANELONI |
work_keys_str_mv |
AT stanelonirj additionofglucosetodolichyldiphosphateoligosaccharideandtransfertoprotein AT ugaldera additionofglucosetodolichyldiphosphateoligosaccharideandtransfertoprotein AT leloirlf additionofglucosetodolichyldiphosphateoligosaccharideandtransfertoprotein |
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