Evidence indicating that Argentine populations of Anastrepha fraterculus (Diptera: Tephritidae) belong to a single biological species
Anastrepha fraterculus (Wiedmann), a South American fruit fly, is an economically important pest for fruit-bearing plants of the neotropical region. Some evidence indicates that the nominal A. fraterculus comprises multiple cryptic species. In the current work we analyzed genetic variability and str...
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todo:paper_00138746_v95_n4_p505_Alberti2023-10-03T14:11:35Z Evidence indicating that Argentine populations of Anastrepha fraterculus (Diptera: Tephritidae) belong to a single biological species Alberti, A.C. Rodriguero, M.S. Cendra, P.G. Saidman, B.O. Vilardi, J.C. Allozymes Anastrepha fraterculus Genetic variability Polymerase chain reaction-restriction fragment-length polymorphism Population structure allozyme polymerase chain reaction population genetics taxonomy Argentina Anastrepha Anastrepha fraterculus Anastrepha fraterculus Argentina (fish) Diptera Diptera Drosophila melanogaster Tephritidae Tephritidae Anastrepha fraterculus (Wiedmann), a South American fruit fly, is an economically important pest for fruit-bearing plants of the neotropical region. Some evidence indicates that the nominal A. fraterculus comprises multiple cryptic species. In the current work we analyzed genetic variability and structure in Argentine populations of A. fraterculus based on eight isoenzymatic loci and the restriction patterns of a fragment of ≈500 bp belonging to the 16 S mitochondrial ribosomal DNA. The isoenzymatic analysis indicated a significant excess of homozygotes (FIS > 0), due to the fact that sampling sites probably do not constitute stable Mendelian populations. Genetic similarities ranged within values expected for conspecific populations and gene flow, estimated indirectly from the FST value, was high. The phenogram obtained by unweighted pair-group method with arithmetic average among populations shows disagreements with the expected result based on geographic distribution. Populations located in the neighborhood of Buenos Aires City show high diversity probably due to a high migration rate from different regions. The polymerase chain reaction (PCR) + restriction fragment-length polymorphism (RFLP) analysis of the mtDNA fragment involved 11 Argentine and one south Brazilian populations. The PCR product was digested by three endonucleases: Ssp-I, Ase-I, and Mnl-I. The observed patterns showed no variation within nor among the populations studied here but differed from the expected results based on the sequences recorded by other researchers for populations of north Brazil, Venezuela, and Mexico. The results so far obtained indicate that, despite the occurrence of macrogeographical variation, the Argentine and south Brazilian populations studied here belong to a single biological species. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00138746_v95_n4_p505_Alberti |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Allozymes Anastrepha fraterculus Genetic variability Polymerase chain reaction-restriction fragment-length polymorphism Population structure allozyme polymerase chain reaction population genetics taxonomy Argentina Anastrepha Anastrepha fraterculus Anastrepha fraterculus Argentina (fish) Diptera Diptera Drosophila melanogaster Tephritidae Tephritidae |
| spellingShingle |
Allozymes Anastrepha fraterculus Genetic variability Polymerase chain reaction-restriction fragment-length polymorphism Population structure allozyme polymerase chain reaction population genetics taxonomy Argentina Anastrepha Anastrepha fraterculus Anastrepha fraterculus Argentina (fish) Diptera Diptera Drosophila melanogaster Tephritidae Tephritidae Alberti, A.C. Rodriguero, M.S. Cendra, P.G. Saidman, B.O. Vilardi, J.C. Evidence indicating that Argentine populations of Anastrepha fraterculus (Diptera: Tephritidae) belong to a single biological species |
| topic_facet |
Allozymes Anastrepha fraterculus Genetic variability Polymerase chain reaction-restriction fragment-length polymorphism Population structure allozyme polymerase chain reaction population genetics taxonomy Argentina Anastrepha Anastrepha fraterculus Anastrepha fraterculus Argentina (fish) Diptera Diptera Drosophila melanogaster Tephritidae Tephritidae |
| description |
Anastrepha fraterculus (Wiedmann), a South American fruit fly, is an economically important pest for fruit-bearing plants of the neotropical region. Some evidence indicates that the nominal A. fraterculus comprises multiple cryptic species. In the current work we analyzed genetic variability and structure in Argentine populations of A. fraterculus based on eight isoenzymatic loci and the restriction patterns of a fragment of ≈500 bp belonging to the 16 S mitochondrial ribosomal DNA. The isoenzymatic analysis indicated a significant excess of homozygotes (FIS > 0), due to the fact that sampling sites probably do not constitute stable Mendelian populations. Genetic similarities ranged within values expected for conspecific populations and gene flow, estimated indirectly from the FST value, was high. The phenogram obtained by unweighted pair-group method with arithmetic average among populations shows disagreements with the expected result based on geographic distribution. Populations located in the neighborhood of Buenos Aires City show high diversity probably due to a high migration rate from different regions. The polymerase chain reaction (PCR) + restriction fragment-length polymorphism (RFLP) analysis of the mtDNA fragment involved 11 Argentine and one south Brazilian populations. The PCR product was digested by three endonucleases: Ssp-I, Ase-I, and Mnl-I. The observed patterns showed no variation within nor among the populations studied here but differed from the expected results based on the sequences recorded by other researchers for populations of north Brazil, Venezuela, and Mexico. The results so far obtained indicate that, despite the occurrence of macrogeographical variation, the Argentine and south Brazilian populations studied here belong to a single biological species. |
| format |
JOUR |
| author |
Alberti, A.C. Rodriguero, M.S. Cendra, P.G. Saidman, B.O. Vilardi, J.C. |
| author_facet |
Alberti, A.C. Rodriguero, M.S. Cendra, P.G. Saidman, B.O. Vilardi, J.C. |
| author_sort |
Alberti, A.C. |
| title |
Evidence indicating that Argentine populations of Anastrepha fraterculus (Diptera: Tephritidae) belong to a single biological species |
| title_short |
Evidence indicating that Argentine populations of Anastrepha fraterculus (Diptera: Tephritidae) belong to a single biological species |
| title_full |
Evidence indicating that Argentine populations of Anastrepha fraterculus (Diptera: Tephritidae) belong to a single biological species |
| title_fullStr |
Evidence indicating that Argentine populations of Anastrepha fraterculus (Diptera: Tephritidae) belong to a single biological species |
| title_full_unstemmed |
Evidence indicating that Argentine populations of Anastrepha fraterculus (Diptera: Tephritidae) belong to a single biological species |
| title_sort |
evidence indicating that argentine populations of anastrepha fraterculus (diptera: tephritidae) belong to a single biological species |
| url |
http://hdl.handle.net/20.500.12110/paper_00138746_v95_n4_p505_Alberti |
| work_keys_str_mv |
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1807321209179734016 |