Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin

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The antiapoptotic effect of melatonin has been described in several systems. In this study, the antagonistic effect of the methoxyindole on dexamethasone-induced apoptosis in mouse thymocytes was examined. Melatonin decreased both DNA fragmentation, and the number of annexin V-positive cells incubat...

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Autores principales:	Hoijman, E., Rocha Viegas, L., Keller Sarmiento, M.I., Rosenstein, R.E., Pecci, A.
Formato:	JOUR
Materias:	cytochrome c dexamethasone DNA fragment glucocorticoid lipocortin 5 melatonin protein Bak protein Bax protein bcl 2 protein bcl xl animal cell animal experiment animal model apoptosis article cell death cellular distribution controlled study drug induced disease enzyme activation gene overexpression male mitochondrial membrane mouse nonhuman outer membrane priority journal protein analysis protein family protein synthesis regulation receptor density thymocyte thymus Adjuvants, Immunologic Animals Apoptosis bcl-2-Associated X Protein Cytochromes c Dexamethasone Glucocorticoids Male Melatonin Mice Mice, Inbred Strains Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Receptors, Glucocorticoid RNA, Messenger Thymus Gland
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00137227_v145_n1_p418_Hoijman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00137227_v145_n1_p418_Hoijman2023-10-03T14:11:17Z Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin Hoijman, E. Rocha Viegas, L. Keller Sarmiento, M.I. Rosenstein, R.E. Pecci, A. cytochrome c dexamethasone DNA fragment glucocorticoid lipocortin 5 melatonin protein Bak protein Bax protein bcl 2 protein bcl xl animal cell animal experiment animal model apoptosis article cell death cellular distribution controlled study drug induced disease enzyme activation gene overexpression male mitochondrial membrane mouse nonhuman outer membrane priority journal protein analysis protein family protein synthesis regulation receptor density thymocyte thymus Adjuvants, Immunologic Animals Apoptosis bcl-2-Associated X Protein Cytochromes c Dexamethasone Glucocorticoids Male Melatonin Mice Mice, Inbred Strains Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Receptors, Glucocorticoid RNA, Messenger Thymus Gland The antiapoptotic effect of melatonin has been described in several systems. In this study, the antagonistic effect of the methoxyindole on dexamethasone-induced apoptosis in mouse thymocytes was examined. Melatonin decreased both DNA fragmentation, and the number of annexin V-positive cells incubated in the presence of dexamethasone. Analysis of the expression of the members of the Bcl-2 family indicated that the synthetic glucocorticoid increased Bax protein levels without affecting the levels of Bcl-2, Bcl-X L, Bcl-X S, or Bak. This effect correlated with an increase in thymocytes bax mRNA levels. Dexamethasone also increased the release of cytochrome C from mitochondria. All of these effects were reduced in the presence of melatonin, which was ineffective per se on these parameters. In addition, the involvement of cAMP on glucocorticoid/melatonin antagonism was examined. Both melatonin and dexamethasone decreased the levels of this nucleotide in mouse thymocytes, indicating that the antagonistic action between both hormones involves a cAMP-independent pathway. In summary, the present results suggest that the antiapoptotic effect of melatonin on glucocorticoid-treated thymocytes would be a consequence of an inhibition of the mitochondrial pathway, presumably through the regulation of Bax protein levels. Fil:Hoijman, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rocha Viegas, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rosenstein, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00137227_v145_n1_p418_Hoijman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cytochrome c dexamethasone DNA fragment glucocorticoid lipocortin 5 melatonin protein Bak protein Bax protein bcl 2 protein bcl xl animal cell animal experiment animal model apoptosis article cell death cellular distribution controlled study drug induced disease enzyme activation gene overexpression male mitochondrial membrane mouse nonhuman outer membrane priority journal protein analysis protein family protein synthesis regulation receptor density thymocyte thymus Adjuvants, Immunologic Animals Apoptosis bcl-2-Associated X Protein Cytochromes c Dexamethasone Glucocorticoids Male Melatonin Mice Mice, Inbred Strains Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Receptors, Glucocorticoid RNA, Messenger Thymus Gland
spellingShingle	cytochrome c dexamethasone DNA fragment glucocorticoid lipocortin 5 melatonin protein Bak protein Bax protein bcl 2 protein bcl xl animal cell animal experiment animal model apoptosis article cell death cellular distribution controlled study drug induced disease enzyme activation gene overexpression male mitochondrial membrane mouse nonhuman outer membrane priority journal protein analysis protein family protein synthesis regulation receptor density thymocyte thymus Adjuvants, Immunologic Animals Apoptosis bcl-2-Associated X Protein Cytochromes c Dexamethasone Glucocorticoids Male Melatonin Mice Mice, Inbred Strains Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Receptors, Glucocorticoid RNA, Messenger Thymus Gland Hoijman, E. Rocha Viegas, L. Keller Sarmiento, M.I. Rosenstein, R.E. Pecci, A. Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin
topic_facet	cytochrome c dexamethasone DNA fragment glucocorticoid lipocortin 5 melatonin protein Bak protein Bax protein bcl 2 protein bcl xl animal cell animal experiment animal model apoptosis article cell death cellular distribution controlled study drug induced disease enzyme activation gene overexpression male mitochondrial membrane mouse nonhuman outer membrane priority journal protein analysis protein family protein synthesis regulation receptor density thymocyte thymus Adjuvants, Immunologic Animals Apoptosis bcl-2-Associated X Protein Cytochromes c Dexamethasone Glucocorticoids Male Melatonin Mice Mice, Inbred Strains Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Receptors, Glucocorticoid RNA, Messenger Thymus Gland
description	The antiapoptotic effect of melatonin has been described in several systems. In this study, the antagonistic effect of the methoxyindole on dexamethasone-induced apoptosis in mouse thymocytes was examined. Melatonin decreased both DNA fragmentation, and the number of annexin V-positive cells incubated in the presence of dexamethasone. Analysis of the expression of the members of the Bcl-2 family indicated that the synthetic glucocorticoid increased Bax protein levels without affecting the levels of Bcl-2, Bcl-X L, Bcl-X S, or Bak. This effect correlated with an increase in thymocytes bax mRNA levels. Dexamethasone also increased the release of cytochrome C from mitochondria. All of these effects were reduced in the presence of melatonin, which was ineffective per se on these parameters. In addition, the involvement of cAMP on glucocorticoid/melatonin antagonism was examined. Both melatonin and dexamethasone decreased the levels of this nucleotide in mouse thymocytes, indicating that the antagonistic action between both hormones involves a cAMP-independent pathway. In summary, the present results suggest that the antiapoptotic effect of melatonin on glucocorticoid-treated thymocytes would be a consequence of an inhibition of the mitochondrial pathway, presumably through the regulation of Bax protein levels.
format	JOUR
author	Hoijman, E. Rocha Viegas, L. Keller Sarmiento, M.I. Rosenstein, R.E. Pecci, A.
author_facet	Hoijman, E. Rocha Viegas, L. Keller Sarmiento, M.I. Rosenstein, R.E. Pecci, A.
author_sort	Hoijman, E.
title	Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin
title_short	Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin
title_full	Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin
title_fullStr	Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin
title_full_unstemmed	Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin
title_sort	involvement of bax protein in the prevention of glucocorticoid-induced thymocytes apoptosis by melatonin
url	http://hdl.handle.net/20.500.12110/paper_00137227_v145_n1_p418_Hoijman
work_keys_str_mv	AT hoijmane involvementofbaxproteininthepreventionofglucocorticoidinducedthymocytesapoptosisbymelatonin AT rochaviegasl involvementofbaxproteininthepreventionofglucocorticoidinducedthymocytesapoptosisbymelatonin AT kellersarmientomi involvementofbaxproteininthepreventionofglucocorticoidinducedthymocytesapoptosisbymelatonin AT rosensteinre involvementofbaxproteininthepreventionofglucocorticoidinducedthymocytesapoptosisbymelatonin AT peccia involvementofbaxproteininthepreventionofglucocorticoidinducedthymocytesapoptosisbymelatonin
_version_	1782030878088626176

Involvement of Bax Protein in the Prevention of Glucocorticoid-Induced Thymocytes Apoptosis by Melatonin

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