Dimeric inhibin A and B production are differentially regulated by hormones and local factors in rat granulosa cells
In the present study, we have examined the role of hormones and growth factors in regulating dimeric inhibin production in immature rat granulosa cells. Purified granulosa cells from estrogen-primed immature rats were cultured under defined conditions. Inhibins A and B in the culture media were meas...
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todo:paper_00137227_v140_n6_p2549_Lanuza2023-10-03T14:11:13Z Dimeric inhibin A and B production are differentially regulated by hormones and local factors in rat granulosa cells Lanuza, G.M. Groome, N.P. Barañao, J.L. Campo, S. activin A estradiol follitropin growth factor hormone inhibin A inhibin B transforming growth factor beta animal cell article cattle coculture concentration response controlled study enzyme linked immunosorbent assay granulosa cell hormonal regulation hormone action hormone sensitivity nonhuman oocyte priority journal protein blood level protein determination protein synthesis rat In the present study, we have examined the role of hormones and growth factors in regulating dimeric inhibin production in immature rat granulosa cells. Purified granulosa cells from estrogen-primed immature rats were cultured under defined conditions. Inhibins A and B in the culture media were measured using a two-site enzyme-linked immunosorbent assay specific for each dimer. Under basal conditions, granulosa cells produced 14-fold more inhibin A than inhibin B (inhibin A, 2.0; inhibin B, 0.14 ng/ml, measured against human standards; average A/B apparent ratio, 14). Addition of increasing doses of FSH elicited dose-dependent increases in both inhibins, the effects being more pronounced on inhibin A than on inhibin B (9.4- and 4.1-fold increases, respectively; average A/B ratio, 34). Estradiol, when added alone, stimulated inhibin A production 3- to 6-fold, whereas minor changes were observed in inhibin B production. Insulin-like growth factor-I produced a similar stimulation of both inhibins (3-fold stimulation over control). This growth factor, however, induced a marked dissociation in the sensitivity of inhibins A and B to FSH stimulation, with maximal stimulation of inhibin B observed at comparatively lower concentrations of the gonadotropin. Transforming growth factor-β (TGF-β, 5 ng/ml) had a more marked stimulatory effect on inhibin B than on inhibin A production (7- to 14-fold vs. 2- to 5-fold for inhibin B and A, respectively). A more pronounced differential stimulation of inhibin B was also exerted by another member of the TGF-β superfamily, activin A (A/B ratio, 0.66). This preferential stimulation of inhibin B by TGF-β and activin A was amplified in the presence of FSH. Coculture of rat granulosa cells with freshly isolated bovine oocytes was also associated with a marked stimulation of inhibin B production (100-fold increase) and a comparatively lower stimulation of inhibin A (10-fold increase; A/B ratio, 1). The discrepancy between the proportion of inhibin dimers in serum (A/B ratio, 0.13) and those produced by untreated granulosa cells may suggest that intraovarian factors, such as TGF-β, activin A, or oocyte-derived factor(s), are responsible for the shift of the ratio toward the predominance of inhibin B. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00137227_v140_n6_p2549_Lanuza |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
activin A estradiol follitropin growth factor hormone inhibin A inhibin B transforming growth factor beta animal cell article cattle coculture concentration response controlled study enzyme linked immunosorbent assay granulosa cell hormonal regulation hormone action hormone sensitivity nonhuman oocyte priority journal protein blood level protein determination protein synthesis rat |
| spellingShingle |
activin A estradiol follitropin growth factor hormone inhibin A inhibin B transforming growth factor beta animal cell article cattle coculture concentration response controlled study enzyme linked immunosorbent assay granulosa cell hormonal regulation hormone action hormone sensitivity nonhuman oocyte priority journal protein blood level protein determination protein synthesis rat Lanuza, G.M. Groome, N.P. Barañao, J.L. Campo, S. Dimeric inhibin A and B production are differentially regulated by hormones and local factors in rat granulosa cells |
| topic_facet |
activin A estradiol follitropin growth factor hormone inhibin A inhibin B transforming growth factor beta animal cell article cattle coculture concentration response controlled study enzyme linked immunosorbent assay granulosa cell hormonal regulation hormone action hormone sensitivity nonhuman oocyte priority journal protein blood level protein determination protein synthesis rat |
| description |
In the present study, we have examined the role of hormones and growth factors in regulating dimeric inhibin production in immature rat granulosa cells. Purified granulosa cells from estrogen-primed immature rats were cultured under defined conditions. Inhibins A and B in the culture media were measured using a two-site enzyme-linked immunosorbent assay specific for each dimer. Under basal conditions, granulosa cells produced 14-fold more inhibin A than inhibin B (inhibin A, 2.0; inhibin B, 0.14 ng/ml, measured against human standards; average A/B apparent ratio, 14). Addition of increasing doses of FSH elicited dose-dependent increases in both inhibins, the effects being more pronounced on inhibin A than on inhibin B (9.4- and 4.1-fold increases, respectively; average A/B ratio, 34). Estradiol, when added alone, stimulated inhibin A production 3- to 6-fold, whereas minor changes were observed in inhibin B production. Insulin-like growth factor-I produced a similar stimulation of both inhibins (3-fold stimulation over control). This growth factor, however, induced a marked dissociation in the sensitivity of inhibins A and B to FSH stimulation, with maximal stimulation of inhibin B observed at comparatively lower concentrations of the gonadotropin. Transforming growth factor-β (TGF-β, 5 ng/ml) had a more marked stimulatory effect on inhibin B than on inhibin A production (7- to 14-fold vs. 2- to 5-fold for inhibin B and A, respectively). A more pronounced differential stimulation of inhibin B was also exerted by another member of the TGF-β superfamily, activin A (A/B ratio, 0.66). This preferential stimulation of inhibin B by TGF-β and activin A was amplified in the presence of FSH. Coculture of rat granulosa cells with freshly isolated bovine oocytes was also associated with a marked stimulation of inhibin B production (100-fold increase) and a comparatively lower stimulation of inhibin A (10-fold increase; A/B ratio, 1). The discrepancy between the proportion of inhibin dimers in serum (A/B ratio, 0.13) and those produced by untreated granulosa cells may suggest that intraovarian factors, such as TGF-β, activin A, or oocyte-derived factor(s), are responsible for the shift of the ratio toward the predominance of inhibin B. |
| format |
JOUR |
| author |
Lanuza, G.M. Groome, N.P. Barañao, J.L. Campo, S. |
| author_facet |
Lanuza, G.M. Groome, N.P. Barañao, J.L. Campo, S. |
| author_sort |
Lanuza, G.M. |
| title |
Dimeric inhibin A and B production are differentially regulated by hormones and local factors in rat granulosa cells |
| title_short |
Dimeric inhibin A and B production are differentially regulated by hormones and local factors in rat granulosa cells |
| title_full |
Dimeric inhibin A and B production are differentially regulated by hormones and local factors in rat granulosa cells |
| title_fullStr |
Dimeric inhibin A and B production are differentially regulated by hormones and local factors in rat granulosa cells |
| title_full_unstemmed |
Dimeric inhibin A and B production are differentially regulated by hormones and local factors in rat granulosa cells |
| title_sort |
dimeric inhibin a and b production are differentially regulated by hormones and local factors in rat granulosa cells |
| url |
http://hdl.handle.net/20.500.12110/paper_00137227_v140_n6_p2549_Lanuza |
| work_keys_str_mv |
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