Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression

The effects of long-term corticotropin-releasing hormone (CRH) infusion in the lateral ventricle of the rat on hypothalamic-pituitary-adrenocortical (HPA) axis parameters and on the immune system function were studied. Compared with infusion of vehicle, the CRH treatment produced a sustained overact...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Labeur, M.S., Arzt, E., Wiegers, G.J., Holsboer, F., Reul, J.M.H.M.
Formato: JOUR
Materias:
concanavalin a
corticosterone
corticotropin
corticotropin releasing factor
cytokine
interleukin 1beta
interleukin 2
interleukin 2 receptor
lipopolysaccharide
messenger rna
proopiomelanocortin
animal cell
animal experiment
article
b lymphocyte
corticotropin blood level
gene expression
hypothalamus hypophysis adrenal system
immune system
intracerebroventricular drug administration
lateral brain ventricle
lymphocyte proliferation
nonhuman
priority journal
rat
spleen cell
t lymphocyte
weight gain
Animal
B-Lymphocytes
Corticotropin-Releasing Hormone
Cytokines
Human
Hypothalamo-Hypophyseal System
In Vitro
Injections, Intraventricular
Interleukin-1
Interleukin-2
Lymphocyte Activation
Male
Neuroimmunomodulation
Pituitary-Adrenal System
Rats
Rats, Wistar
Receptors, Interleukin-2
RNA, Messenger
Support, Non-U.S. Gov't
T-Lymphocytes
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00137227_v136_n6_p2678_Labeur
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00137227_v136_n6_p2678_Labeur
record_format dspace
spelling todo:paper_00137227_v136_n6_p2678_Labeur2023-10-03T14:11:10Z Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression Labeur, M.S. Arzt, E. Wiegers, G.J. Holsboer, F. Reul, J.M.H.M. concanavalin a corticosterone corticotropin corticotropin releasing factor cytokine interleukin 1beta interleukin 2 interleukin 2 receptor lipopolysaccharide messenger rna proopiomelanocortin animal cell animal experiment article b lymphocyte corticotropin blood level gene expression hypothalamus hypophysis adrenal system immune system intracerebroventricular drug administration lateral brain ventricle lymphocyte proliferation nonhuman priority journal rat spleen cell t lymphocyte weight gain Animal B-Lymphocytes Corticotropin-Releasing Hormone Cytokines Human Hypothalamo-Hypophyseal System In Vitro Injections, Intraventricular Interleukin-1 Interleukin-2 Lymphocyte Activation Male Neuroimmunomodulation Pituitary-Adrenal System Rats Rats, Wistar Receptors, Interleukin-2 RNA, Messenger Support, Non-U.S. Gov't T-Lymphocytes The effects of long-term corticotropin-releasing hormone (CRH) infusion in the lateral ventricle of the rat on hypothalamic-pituitary-adrenocortical (HPA) axis parameters and on the immune system function were studied. Compared with infusion of vehicle, the CRH treatment produced a sustained overactivity of the HPA axis, as evidenced by elevated plasma ACTH and corticosterone levels, increased anterior pituitary POMC messenger RNA (mRNA) expression, and adrenal enlargement. Long-term CRH treatment also inhibited body weight gain and reduced thymus and spleen weight. In the CRH-treated animals, both Concanavalin A (Con A)-induced T lymphocyte proliferation and lipopolysaccharide (LPS)-induced B lymphocyte mitogenesis was largely suppressed. Surprisingly, interleukin-2 (IL-2) levels were higher in supernatants of splenocyte cultures from CRH-treated rats than in those of control animals. However, IL-2 receptor alpha chain (IL-2R α) mRNA expression after Con A stimulation was highly suppressed in the CRH-treated animals. In addition, Northern blot analysis of RNA from splenocytes isolated from spleens of CRH-treated rats revealed a marked expression of IL-1 β mRNA, in contrast to the barely detectable levels of this cytokine in control animals. Moreover, incubation of total splenocytes and spleen macrophages with LPS resulted in an enhanced induction of IL-1 β mRNA in cells of CRH-treated rats compared with that of control animals. When adrenalectomized rats were treated with CRH or vehicle, the effects of the CRH treatment on T and B cell proliferation, IL-2 production, and IL-1 β mRNA expression were abolished. Thus, a continuously increased HPA axis drive results in disparate changes in immune system function. Whether the observed changes in cytokine expression should be regarded as physiologically adaptive adjustments in support of immune function or as potentially pathological anomalies remains to be elucidated. © 1995 by The Endocrine Society. Fil:Labeur, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00137227_v136_n6_p2678_Labeur
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic concanavalin a
corticosterone
corticotropin
corticotropin releasing factor
cytokine
interleukin 1beta
interleukin 2
interleukin 2 receptor
lipopolysaccharide
messenger rna
proopiomelanocortin
animal cell
animal experiment
article
b lymphocyte
corticotropin blood level
gene expression
hypothalamus hypophysis adrenal system
immune system
intracerebroventricular drug administration
lateral brain ventricle
lymphocyte proliferation
nonhuman
priority journal
rat
spleen cell
t lymphocyte
weight gain
Animal
B-Lymphocytes
Corticotropin-Releasing Hormone
Cytokines
Human
Hypothalamo-Hypophyseal System
In Vitro
Injections, Intraventricular
Interleukin-1
Interleukin-2
Lymphocyte Activation
Male
Neuroimmunomodulation
Pituitary-Adrenal System
Rats
Rats, Wistar
Receptors, Interleukin-2
RNA, Messenger
Support, Non-U.S. Gov't
T-Lymphocytes
spellingShingle concanavalin a
corticosterone
corticotropin
corticotropin releasing factor
cytokine
interleukin 1beta
interleukin 2
interleukin 2 receptor
lipopolysaccharide
messenger rna
proopiomelanocortin
animal cell
animal experiment
article
b lymphocyte
corticotropin blood level
gene expression
hypothalamus hypophysis adrenal system
immune system
intracerebroventricular drug administration
lateral brain ventricle
lymphocyte proliferation
nonhuman
priority journal
rat
spleen cell
t lymphocyte
weight gain
Animal
B-Lymphocytes
Corticotropin-Releasing Hormone
Cytokines
Human
Hypothalamo-Hypophyseal System
In Vitro
Injections, Intraventricular
Interleukin-1
Interleukin-2
Lymphocyte Activation
Male
Neuroimmunomodulation
Pituitary-Adrenal System
Rats
Rats, Wistar
Receptors, Interleukin-2
RNA, Messenger
Support, Non-U.S. Gov't
T-Lymphocytes
Labeur, M.S.
Arzt, E.
Wiegers, G.J.
Holsboer, F.
Reul, J.M.H.M.
Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression
topic_facet concanavalin a
corticosterone
corticotropin
corticotropin releasing factor
cytokine
interleukin 1beta
interleukin 2
interleukin 2 receptor
lipopolysaccharide
messenger rna
proopiomelanocortin
animal cell
animal experiment
article
b lymphocyte
corticotropin blood level
gene expression
hypothalamus hypophysis adrenal system
immune system
intracerebroventricular drug administration
lateral brain ventricle
lymphocyte proliferation
nonhuman
priority journal
rat
spleen cell
t lymphocyte
weight gain
Animal
B-Lymphocytes
Corticotropin-Releasing Hormone
Cytokines
Human
Hypothalamo-Hypophyseal System
In Vitro
Injections, Intraventricular
Interleukin-1
Interleukin-2
Lymphocyte Activation
Male
Neuroimmunomodulation
Pituitary-Adrenal System
Rats
Rats, Wistar
Receptors, Interleukin-2
RNA, Messenger
Support, Non-U.S. Gov't
T-Lymphocytes
description The effects of long-term corticotropin-releasing hormone (CRH) infusion in the lateral ventricle of the rat on hypothalamic-pituitary-adrenocortical (HPA) axis parameters and on the immune system function were studied. Compared with infusion of vehicle, the CRH treatment produced a sustained overactivity of the HPA axis, as evidenced by elevated plasma ACTH and corticosterone levels, increased anterior pituitary POMC messenger RNA (mRNA) expression, and adrenal enlargement. Long-term CRH treatment also inhibited body weight gain and reduced thymus and spleen weight. In the CRH-treated animals, both Concanavalin A (Con A)-induced T lymphocyte proliferation and lipopolysaccharide (LPS)-induced B lymphocyte mitogenesis was largely suppressed. Surprisingly, interleukin-2 (IL-2) levels were higher in supernatants of splenocyte cultures from CRH-treated rats than in those of control animals. However, IL-2 receptor alpha chain (IL-2R α) mRNA expression after Con A stimulation was highly suppressed in the CRH-treated animals. In addition, Northern blot analysis of RNA from splenocytes isolated from spleens of CRH-treated rats revealed a marked expression of IL-1 β mRNA, in contrast to the barely detectable levels of this cytokine in control animals. Moreover, incubation of total splenocytes and spleen macrophages with LPS resulted in an enhanced induction of IL-1 β mRNA in cells of CRH-treated rats compared with that of control animals. When adrenalectomized rats were treated with CRH or vehicle, the effects of the CRH treatment on T and B cell proliferation, IL-2 production, and IL-1 β mRNA expression were abolished. Thus, a continuously increased HPA axis drive results in disparate changes in immune system function. Whether the observed changes in cytokine expression should be regarded as physiologically adaptive adjustments in support of immune function or as potentially pathological anomalies remains to be elucidated. © 1995 by The Endocrine Society.
format JOUR
author Labeur, M.S.
Arzt, E.
Wiegers, G.J.
Holsboer, F.
Reul, J.M.H.M.
author_facet Labeur, M.S.
Arzt, E.
Wiegers, G.J.
Holsboer, F.
Reul, J.M.H.M.
author_sort Labeur, M.S.
title Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression
title_short Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression
title_full Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression
title_fullStr Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression
title_full_unstemmed Long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression
title_sort long-term intracerebroventricular corticotropin-releasing hormone administration induces distinct changes in rat splenocyte activation and cytokine expression
url http://hdl.handle.net/20.500.12110/paper_00137227_v136_n6_p2678_Labeur
work_keys_str_mv AT labeurms longtermintracerebroventricularcorticotropinreleasinghormoneadministrationinducesdistinctchangesinratsplenocyteactivationandcytokineexpression
AT arzte longtermintracerebroventricularcorticotropinreleasinghormoneadministrationinducesdistinctchangesinratsplenocyteactivationandcytokineexpression
AT wiegersgj longtermintracerebroventricularcorticotropinreleasinghormoneadministrationinducesdistinctchangesinratsplenocyteactivationandcytokineexpression
AT holsboerf longtermintracerebroventricularcorticotropinreleasinghormoneadministrationinducesdistinctchangesinratsplenocyteactivationandcytokineexpression
AT reuljmhm longtermintracerebroventricularcorticotropinreleasinghormoneadministrationinducesdistinctchangesinratsplenocyteactivationandcytokineexpression
_version_ 1807317754502447104

Ejemplares similares