Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin
The interleukin-1 (IL-1) system is constituted by IL-1 alpha and IL-1 β and IL-1 receptor antagonist (IL-1ra) that bind the same IL-1 receptors. Hypothalamic-pituitary-adrenal axis hormones are major mediators of the neuroendocrine control over immune function. Corticotropin-releasing hormone (CRH)...
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todo:paper_00137227_v136_n12_p5504_Pereda2023-10-03T14:11:10Z Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin Pereda, M.P. Sauer, J. Castro, C.P. Finkielman, S. Stalla, G.K. Holsboer, F. Arzt, E. corticotropin releasing factor interleukin 1 interleukin receptor article corticotropin release human human cell hypothalamus hypophysis adrenal system leukocyte activation priority journal protein synthesis Cells, Cultured Corticotropin-Releasing Hormone Cyclic AMP Gene Expression Regulation Human Interleukin-1 Lipopolysaccharides Monocytes RNA, Messenger Sialoglycoproteins Support, Non-U.S. Gov't The interleukin-1 (IL-1) system is constituted by IL-1 alpha and IL-1 β and IL-1 receptor antagonist (IL-1ra) that bind the same IL-1 receptors. Hypothalamic-pituitary-adrenal axis hormones are major mediators of the neuroendocrine control over immune function. Corticotropin-releasing hormone (CRH) is produced in peripheral inflammatory sites; its direct effects on inflammatory cytokine synthesis, however, remain unclear. We have studied the effects of CRH (0.1-100 nM) on IL-1 β and IL-1ra expression by human peripheral monocytes in culture activated with different doses of lipopolysaccharide (LPS). In the absence of LPS, CRH up-regulated IL-1ra and IL-1 β messenger RNA expression as well as protein synthesis. No significant changes were observed with low doses of LPS (1 ng/ml). In contrast, in combination with high doses of LPS (1 μ/ml), CRH caused inhibition of IL-1ra and IL-1 β transcription and secretion. The CRH effects were blocked by its antagonist alpha-helical CRH and mediated by intracellular cAMP. These data indicate that CRH modulates the IL-1 system; depending on the state of activation of the monocyte, CRH exerts an inhibitory control on the activated cell and a stimulatory action on the resting monocyte. © 1995 by The Endocrine Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00137227_v136_n12_p5504_Pereda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
corticotropin releasing factor interleukin 1 interleukin receptor article corticotropin release human human cell hypothalamus hypophysis adrenal system leukocyte activation priority journal protein synthesis Cells, Cultured Corticotropin-Releasing Hormone Cyclic AMP Gene Expression Regulation Human Interleukin-1 Lipopolysaccharides Monocytes RNA, Messenger Sialoglycoproteins Support, Non-U.S. Gov't |
| spellingShingle |
corticotropin releasing factor interleukin 1 interleukin receptor article corticotropin release human human cell hypothalamus hypophysis adrenal system leukocyte activation priority journal protein synthesis Cells, Cultured Corticotropin-Releasing Hormone Cyclic AMP Gene Expression Regulation Human Interleukin-1 Lipopolysaccharides Monocytes RNA, Messenger Sialoglycoproteins Support, Non-U.S. Gov't Pereda, M.P. Sauer, J. Castro, C.P. Finkielman, S. Stalla, G.K. Holsboer, F. Arzt, E. Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin |
| topic_facet |
corticotropin releasing factor interleukin 1 interleukin receptor article corticotropin release human human cell hypothalamus hypophysis adrenal system leukocyte activation priority journal protein synthesis Cells, Cultured Corticotropin-Releasing Hormone Cyclic AMP Gene Expression Regulation Human Interleukin-1 Lipopolysaccharides Monocytes RNA, Messenger Sialoglycoproteins Support, Non-U.S. Gov't |
| description |
The interleukin-1 (IL-1) system is constituted by IL-1 alpha and IL-1 β and IL-1 receptor antagonist (IL-1ra) that bind the same IL-1 receptors. Hypothalamic-pituitary-adrenal axis hormones are major mediators of the neuroendocrine control over immune function. Corticotropin-releasing hormone (CRH) is produced in peripheral inflammatory sites; its direct effects on inflammatory cytokine synthesis, however, remain unclear. We have studied the effects of CRH (0.1-100 nM) on IL-1 β and IL-1ra expression by human peripheral monocytes in culture activated with different doses of lipopolysaccharide (LPS). In the absence of LPS, CRH up-regulated IL-1ra and IL-1 β messenger RNA expression as well as protein synthesis. No significant changes were observed with low doses of LPS (1 ng/ml). In contrast, in combination with high doses of LPS (1 μ/ml), CRH caused inhibition of IL-1ra and IL-1 β transcription and secretion. The CRH effects were blocked by its antagonist alpha-helical CRH and mediated by intracellular cAMP. These data indicate that CRH modulates the IL-1 system; depending on the state of activation of the monocyte, CRH exerts an inhibitory control on the activated cell and a stimulatory action on the resting monocyte. © 1995 by The Endocrine Society. |
| format |
JOUR |
| author |
Pereda, M.P. Sauer, J. Castro, C.P. Finkielman, S. Stalla, G.K. Holsboer, F. Arzt, E. |
| author_facet |
Pereda, M.P. Sauer, J. Castro, C.P. Finkielman, S. Stalla, G.K. Holsboer, F. Arzt, E. |
| author_sort |
Pereda, M.P. |
| title |
Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin |
| title_short |
Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin |
| title_full |
Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin |
| title_fullStr |
Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin |
| title_full_unstemmed |
Corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin |
| title_sort |
corticotropin-releasing hormone differentially modulates the interleukin-1 system according to the level of monocyte activation by endotoxin |
| url |
http://hdl.handle.net/20.500.12110/paper_00137227_v136_n12_p5504_Pereda |
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