Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone
The effect of streptozotocin-induced diabetes on circulating levels of immunoactive LH (I-LH) and bioactive LH (B-LH) was investigated. LH was measured in adult ovariectomized (OVX) rats before and after acute LHRH administration, with or without estradiol benzoate (Eb) treatment (10 μg, 48 and 24...
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todo:paper_00137227_v119_n6_p2412_Tesone2023-10-03T14:11:09Z Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone Tesone, M. Ladenheim, R.G. Cheb-Terrab, R. Chiauzzi, V. Solano, A. Podesta, E. Charreau, E.H. estradiol estradiol benzoate gonadorelin isophane insulin luteinizing hormone streptozocin animal experiment animal model central nervous system drug activity drug efficacy endocrine system female genital system immunity intravenous drug administration methodology nonhuman ovariectomy priority journal rat streptozocin diabetes subcutaneous drug administration Animals Biological Assay Diabetes Mellitus, Experimental Estradiol Female Gonadotropin-Releasing Hormone Immunoassay Insulin Luteinizing Hormone Ovariectomy Pituitary Gland Rats Streptozocin The effect of streptozotocin-induced diabetes on circulating levels of immunoactive LH (I-LH) and bioactive LH (B-LH) was investigated. LH was measured in adult ovariectomized (OVX) rats before and after acute LHRH administration, with or without estradiol benzoate (Eb) treatment (10 μg, 48 and 24 h before experiments). I-LH and B-LH were measured in the same samples by RIA and the rat interstitial cell testosterone assay, respectively. OVX diabetic animals showed a significant reduction in both I-LH (63%) and B-LH (73%). Treatment with Eb induced a decrease in basal I-LH and B-LH levels in all experimental groups (50%). These values were normalized after insulin therapy. No alterations in the pituitary responsiveness to LHRH were detected when I-LH levels were determined. However, B-LH levels assayed after LHRH stimulation were significantly decreased in diabetic animals. Insulin treatment was unable to restore this response. The effect of Eb treatment on these parameters was also tested. In these conditions LHRH injections induced similar increases in serum I-LH and B-LH in both diabetic and control rats. These results indicate that, in diabetic OVX rats, basal and LHRH-induced LH has a reduced bioactivity, but this reduction is reversed by Eb treatment. This might indicate that the major defect lies in the ovary rather than at the pituitary level, supporting the notion of an important role of the steroid milieu on the B-LH modulation. © 1986 by The Endocrine Society. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ladenheim, R.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00137227_v119_n6_p2412_Tesone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
estradiol estradiol benzoate gonadorelin isophane insulin luteinizing hormone streptozocin animal experiment animal model central nervous system drug activity drug efficacy endocrine system female genital system immunity intravenous drug administration methodology nonhuman ovariectomy priority journal rat streptozocin diabetes subcutaneous drug administration Animals Biological Assay Diabetes Mellitus, Experimental Estradiol Female Gonadotropin-Releasing Hormone Immunoassay Insulin Luteinizing Hormone Ovariectomy Pituitary Gland Rats Streptozocin |
| spellingShingle |
estradiol estradiol benzoate gonadorelin isophane insulin luteinizing hormone streptozocin animal experiment animal model central nervous system drug activity drug efficacy endocrine system female genital system immunity intravenous drug administration methodology nonhuman ovariectomy priority journal rat streptozocin diabetes subcutaneous drug administration Animals Biological Assay Diabetes Mellitus, Experimental Estradiol Female Gonadotropin-Releasing Hormone Immunoassay Insulin Luteinizing Hormone Ovariectomy Pituitary Gland Rats Streptozocin Tesone, M. Ladenheim, R.G. Cheb-Terrab, R. Chiauzzi, V. Solano, A. Podesta, E. Charreau, E.H. Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone |
| topic_facet |
estradiol estradiol benzoate gonadorelin isophane insulin luteinizing hormone streptozocin animal experiment animal model central nervous system drug activity drug efficacy endocrine system female genital system immunity intravenous drug administration methodology nonhuman ovariectomy priority journal rat streptozocin diabetes subcutaneous drug administration Animals Biological Assay Diabetes Mellitus, Experimental Estradiol Female Gonadotropin-Releasing Hormone Immunoassay Insulin Luteinizing Hormone Ovariectomy Pituitary Gland Rats Streptozocin |
| description |
The effect of streptozotocin-induced diabetes on circulating levels of immunoactive LH (I-LH) and bioactive LH (B-LH) was investigated. LH was measured in adult ovariectomized (OVX) rats before and after acute LHRH administration, with or without estradiol benzoate (Eb) treatment (10 μg, 48 and 24 h before experiments). I-LH and B-LH were measured in the same samples by RIA and the rat interstitial cell testosterone assay, respectively. OVX diabetic animals showed a significant reduction in both I-LH (63%) and B-LH (73%). Treatment with Eb induced a decrease in basal I-LH and B-LH levels in all experimental groups (50%). These values were normalized after insulin therapy. No alterations in the pituitary responsiveness to LHRH were detected when I-LH levels were determined. However, B-LH levels assayed after LHRH stimulation were significantly decreased in diabetic animals. Insulin treatment was unable to restore this response. The effect of Eb treatment on these parameters was also tested. In these conditions LHRH injections induced similar increases in serum I-LH and B-LH in both diabetic and control rats. These results indicate that, in diabetic OVX rats, basal and LHRH-induced LH has a reduced bioactivity, but this reduction is reversed by Eb treatment. This might indicate that the major defect lies in the ovary rather than at the pituitary level, supporting the notion of an important role of the steroid milieu on the B-LH modulation. © 1986 by The Endocrine Society. |
| format |
JOUR |
| author |
Tesone, M. Ladenheim, R.G. Cheb-Terrab, R. Chiauzzi, V. Solano, A. Podesta, E. Charreau, E.H. |
| author_facet |
Tesone, M. Ladenheim, R.G. Cheb-Terrab, R. Chiauzzi, V. Solano, A. Podesta, E. Charreau, E.H. |
| author_sort |
Tesone, M. |
| title |
Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone |
| title_short |
Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone |
| title_full |
Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone |
| title_fullStr |
Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone |
| title_full_unstemmed |
Comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: Response to lh-releasing hormone |
| title_sort |
comparison between bioactive and immunoactive luteinizing hormone (lh) in ovariectomized streptozotocin-induced diabetic rats: response to lh-releasing hormone |
| url |
http://hdl.handle.net/20.500.12110/paper_00137227_v119_n6_p2412_Tesone |
| work_keys_str_mv |
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