Androgen receptors in the diabetic rat

Male rats rendered diabetic by IV streptozotocin (65mg/kg body weight) were treated with exogenous insulin or testosterone. Charcoal-coated dextran and polyacrylemide gel electrophoresis techniques were employed in studying the characteristics of androgen (R1881) binding to prostate cytosol protein....

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Autores principales:	Tesone, M., Oliveira-Filho, R.M., Biella de Souza Valle, L., Calvo, J.C., Barañao, J.L.S., Foglia, V.G., Charreau, E.H.
Formato:	JOUR
Materias:	Androgen receptors cytosol binding sites R1881 rat prostate streptozotocin-diabetes androgen receptor androstanolone androstanolone h 3 drug receptor insulin methyltrienolone h 3 metribolone radioisotope streptozocin testosterone unclassified drug animal experiment article diabetes mellitus endocrine system male genital system prostate rat Animals Body Weight Cytosol Diabetes Mellitus, Experimental Epididymis Estrenes Insulin, Long-Acting Male Metribolone Organ Size Prostate Rats Receptors, Androgen Receptors, Steroid Testis Testosterone Testosterone Congeners
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0012186X_v18_n5_p385_Tesone
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_0012186X_v18_n5_p385_Tesone2023-10-03T14:10:18Z Androgen receptors in the diabetic rat Tesone, M. Oliveira-Filho, R.M. Biella de Souza Valle, L. Calvo, J.C. Barañao, J.L.S. Foglia, V.G. Charreau, E.H. Androgen receptors cytosol binding sites R1881 rat prostate streptozotocin-diabetes androgen receptor androstanolone androstanolone h 3 drug receptor insulin methyltrienolone h 3 metribolone radioisotope streptozocin testosterone unclassified drug animal experiment article diabetes mellitus endocrine system male genital system prostate rat Animals Body Weight Cytosol Diabetes Mellitus, Experimental Epididymis Estrenes Insulin, Long-Acting Male Metribolone Organ Size Prostate Rats Receptors, Androgen Receptors, Steroid Testis Testosterone Testosterone Congeners Male rats rendered diabetic by IV streptozotocin (65mg/kg body weight) were treated with exogenous insulin or testosterone. Charcoal-coated dextran and polyacrylemide gel electrophoresis techniques were employed in studying the characteristics of androgen (R1881) binding to prostate cytosol protein. In comparison with normal (N) rats, the replacement therapy of diabetic (D) animals with insulin (D+I) or testosterone (D+T) was able to restore epididymal weight (N = 0.40 ±0.04 g; D = 0.18 ± 0.02 g; D+I = 0.42 ± 0.05 g; D+T = 0.40 ± 0.06 g) and total prostate weight (N = 0.24 ± 0.02 g; D = 0.15 ± 0.02 g; D+I = 0.24 ± 0.05 g; D+T = 0.35 ± 0.06 g). Testicular endogenous content of testosterone was restored after insulin treatment (N = 154 ± 13 ng/testis; D = 41 ± 5 ng/testis; D+I = 142 ± 9 ng/testis), and significant improvements of serum testosterone levels were also achieved (N = 540 ± 64 ng/100 ml; D = 238 ± 37 ng/100 ml; D+I = 358 ± 18 ng/100 ml). Prostate cytosol of streptozotocin-diabetic rats had strongly lowered capacity for 3H-R1881 binding compared with controls (94 and 12 fmol/mg protein, respectively). Testosterone treatment produced a 3.3-fold improvement of this lowered value, whereas the increment seen with insulin was less (1.5-fold). It is emphasized that some of the improvements caused by insulin replacement therapy in diabetic animals are due to the partial restoration of testosterone secretion. Thus, the combined actions of insulin and testosterone (instead of insulin alone) seem to be of major importance in the maintenance and regulation of accessory sex glands function. © 1980 Springer-Verlag. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0012186X_v18_n5_p385_Tesone
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Androgen receptors cytosol binding sites R1881 rat prostate streptozotocin-diabetes androgen receptor androstanolone androstanolone h 3 drug receptor insulin methyltrienolone h 3 metribolone radioisotope streptozocin testosterone unclassified drug animal experiment article diabetes mellitus endocrine system male genital system prostate rat Animals Body Weight Cytosol Diabetes Mellitus, Experimental Epididymis Estrenes Insulin, Long-Acting Male Metribolone Organ Size Prostate Rats Receptors, Androgen Receptors, Steroid Testis Testosterone Testosterone Congeners
spellingShingle	Androgen receptors cytosol binding sites R1881 rat prostate streptozotocin-diabetes androgen receptor androstanolone androstanolone h 3 drug receptor insulin methyltrienolone h 3 metribolone radioisotope streptozocin testosterone unclassified drug animal experiment article diabetes mellitus endocrine system male genital system prostate rat Animals Body Weight Cytosol Diabetes Mellitus, Experimental Epididymis Estrenes Insulin, Long-Acting Male Metribolone Organ Size Prostate Rats Receptors, Androgen Receptors, Steroid Testis Testosterone Testosterone Congeners Tesone, M. Oliveira-Filho, R.M. Biella de Souza Valle, L. Calvo, J.C. Barañao, J.L.S. Foglia, V.G. Charreau, E.H. Androgen receptors in the diabetic rat
topic_facet	Androgen receptors cytosol binding sites R1881 rat prostate streptozotocin-diabetes androgen receptor androstanolone androstanolone h 3 drug receptor insulin methyltrienolone h 3 metribolone radioisotope streptozocin testosterone unclassified drug animal experiment article diabetes mellitus endocrine system male genital system prostate rat Animals Body Weight Cytosol Diabetes Mellitus, Experimental Epididymis Estrenes Insulin, Long-Acting Male Metribolone Organ Size Prostate Rats Receptors, Androgen Receptors, Steroid Testis Testosterone Testosterone Congeners
description	Male rats rendered diabetic by IV streptozotocin (65mg/kg body weight) were treated with exogenous insulin or testosterone. Charcoal-coated dextran and polyacrylemide gel electrophoresis techniques were employed in studying the characteristics of androgen (R1881) binding to prostate cytosol protein. In comparison with normal (N) rats, the replacement therapy of diabetic (D) animals with insulin (D+I) or testosterone (D+T) was able to restore epididymal weight (N = 0.40 ±0.04 g; D = 0.18 ± 0.02 g; D+I = 0.42 ± 0.05 g; D+T = 0.40 ± 0.06 g) and total prostate weight (N = 0.24 ± 0.02 g; D = 0.15 ± 0.02 g; D+I = 0.24 ± 0.05 g; D+T = 0.35 ± 0.06 g). Testicular endogenous content of testosterone was restored after insulin treatment (N = 154 ± 13 ng/testis; D = 41 ± 5 ng/testis; D+I = 142 ± 9 ng/testis), and significant improvements of serum testosterone levels were also achieved (N = 540 ± 64 ng/100 ml; D = 238 ± 37 ng/100 ml; D+I = 358 ± 18 ng/100 ml). Prostate cytosol of streptozotocin-diabetic rats had strongly lowered capacity for 3H-R1881 binding compared with controls (94 and 12 fmol/mg protein, respectively). Testosterone treatment produced a 3.3-fold improvement of this lowered value, whereas the increment seen with insulin was less (1.5-fold). It is emphasized that some of the improvements caused by insulin replacement therapy in diabetic animals are due to the partial restoration of testosterone secretion. Thus, the combined actions of insulin and testosterone (instead of insulin alone) seem to be of major importance in the maintenance and regulation of accessory sex glands function. © 1980 Springer-Verlag.
format	JOUR
author	Tesone, M. Oliveira-Filho, R.M. Biella de Souza Valle, L. Calvo, J.C. Barañao, J.L.S. Foglia, V.G. Charreau, E.H.
author_facet	Tesone, M. Oliveira-Filho, R.M. Biella de Souza Valle, L. Calvo, J.C. Barañao, J.L.S. Foglia, V.G. Charreau, E.H.
author_sort	Tesone, M.
title	Androgen receptors in the diabetic rat
title_short	Androgen receptors in the diabetic rat
title_full	Androgen receptors in the diabetic rat
title_fullStr	Androgen receptors in the diabetic rat
title_full_unstemmed	Androgen receptors in the diabetic rat
title_sort	androgen receptors in the diabetic rat
url	http://hdl.handle.net/20.500.12110/paper_0012186X_v18_n5_p385_Tesone
work_keys_str_mv	AT tesonem androgenreceptorsinthediabeticrat AT oliveirafilhorm androgenreceptorsinthediabeticrat AT bielladesouzavallel androgenreceptorsinthediabeticrat AT calvojc androgenreceptorsinthediabeticrat AT baranaojls androgenreceptorsinthediabeticrat AT fogliavg androgenreceptorsinthediabeticrat AT charreaueh androgenreceptorsinthediabeticrat
_version_	1807315058891423744

Androgen receptors in the diabetic rat

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