Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)

The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed,...

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Autores principales:	Rodríguez, M.C., Merino, E.R., Pujol, C.A., Damonte, E.B., Cerezo, A.S., Matulewicz, M.C.
Formato:	JOUR
Materias:	Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00086215_v340_n18_p2742_Rodriguez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00086215_v340_n18_p2742_Rodriguez
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spelling	todo:paper_00086215_v340_n18_p2742_Rodriguez2023-10-03T14:07:08Z Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales) Rodríguez, M.C. Merino, E.R. Pujol, C.A. Damonte, E.B. Cerezo, A.S. Matulewicz, M.C. Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Antiviral activity Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Galactans Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Callophyllis variegata Dengue virus Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Kallymeniaceae The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed, and as the first one contained very high amounts of protein, it was not studied further. Structural analysis of the rest of the fractions (F1-F3) was carried out by methylation, desulfation-methylation, IR, and 13C NMR spectroscopy. The results are consistent for F1 with a carrageenan-type backbone mainly constituted by β-D-galactose 2-sulfate linked to α-D-galactose 2,3,6-trisulfate and β-D-galactose 2,4-disulfate linked to 3,6-anhydro-D-galactose 2-sulfate as dominant diads. In F2 these diads are present together with low amounts of β-D-galactose 2-sulfate linked to 3,6-anhydro-D-galactose 2-sulfate, whose contribution becomes higher in F3. In addition, minor but significant amounts of L-galactose were detected. F1-F3 showed potent antiviral activity against herpes simplex types 1 and 2 and dengue type 2. © 2005 Elsevier Ltd. All rights reserved. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00086215_v340_n18_p2742_Rodriguez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Antiviral activity Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Galactans Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Callophyllis variegata Dengue virus Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Kallymeniaceae
spellingShingle	Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Antiviral activity Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Galactans Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Callophyllis variegata Dengue virus Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Kallymeniaceae Rodríguez, M.C. Merino, E.R. Pujol, C.A. Damonte, E.B. Cerezo, A.S. Matulewicz, M.C. Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
topic_facet	Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Antiviral activity Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Galactans Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Callophyllis variegata Dengue virus Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Kallymeniaceae
description	The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed, and as the first one contained very high amounts of protein, it was not studied further. Structural analysis of the rest of the fractions (F1-F3) was carried out by methylation, desulfation-methylation, IR, and 13C NMR spectroscopy. The results are consistent for F1 with a carrageenan-type backbone mainly constituted by β-D-galactose 2-sulfate linked to α-D-galactose 2,3,6-trisulfate and β-D-galactose 2,4-disulfate linked to 3,6-anhydro-D-galactose 2-sulfate as dominant diads. In F2 these diads are present together with low amounts of β-D-galactose 2-sulfate linked to 3,6-anhydro-D-galactose 2-sulfate, whose contribution becomes higher in F3. In addition, minor but significant amounts of L-galactose were detected. F1-F3 showed potent antiviral activity against herpes simplex types 1 and 2 and dengue type 2. © 2005 Elsevier Ltd. All rights reserved.
format	JOUR
author	Rodríguez, M.C. Merino, E.R. Pujol, C.A. Damonte, E.B. Cerezo, A.S. Matulewicz, M.C.
author_facet	Rodríguez, M.C. Merino, E.R. Pujol, C.A. Damonte, E.B. Cerezo, A.S. Matulewicz, M.C.
author_sort	Rodríguez, M.C.
title	Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_short	Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_full	Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_fullStr	Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_full_unstemmed	Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_sort	galactans from cystocarpic plants of the red seaweed callophyllis variegata (kallymeniaceae, gigartinales)
url	http://hdl.handle.net/20.500.12110/paper_00086215_v340_n18_p2742_Rodriguez
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Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)

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