NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos

Exhaustive extraction of the endosperm from the seed of Gleditsia triacanthos using water at room temperature and 50°C left a residue, which was further extracted at 95°C. Precipitation of this extract with 2-propanol yielded major amounts of galactomannan components, while the supernatant was mainl...

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Autores principales:	Navarro, D.A., Cerezo, A.S., Stortz, C.A.
Formato:	JOUR
Materias:	β-(1 → 4)-Galactan Arabinan Galactomannan Gleditsia triacanthos Legume seeds NMR Chemical analysis Ionic strength Nuclear magnetic resonance spectroscopy Precipitation (chemical) Seed Room temperature Polysaccharides 2 propanol galactomannan galactose oligosaccharide water anion exchange chromatography article chemical analysis controlled study endosperm extraction nonhuman nuclear magnetic resonance spectroscopy precipitation priority journal room temperature seed morphology supernatant Fabaceae Galactans Magnetic Resonance Spectroscopy Mannans Seeds Gleditsia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00086215_v337_n3_p255_Navarro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00086215_v337_n3_p255_Navarro
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spelling	todo:paper_00086215_v337_n3_p255_Navarro2023-10-03T14:07:01Z NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos Navarro, D.A. Cerezo, A.S. Stortz, C.A. β-(1 → 4)-Galactan Arabinan Galactomannan Gleditsia triacanthos Legume seeds NMR Chemical analysis Ionic strength Nuclear magnetic resonance spectroscopy Precipitation (chemical) Seed Room temperature Polysaccharides 2 propanol galactomannan galactose oligosaccharide water anion exchange chromatography article chemical analysis controlled study endosperm extraction nonhuman nuclear magnetic resonance spectroscopy precipitation priority journal room temperature seed morphology supernatant Fabaceae Galactans Magnetic Resonance Spectroscopy Mannans Polysaccharides Seeds Gleditsia Gleditsia triacanthos Exhaustive extraction of the endosperm from the seed of Gleditsia triacanthos using water at room temperature and 50°C left a residue, which was further extracted at 95°C. Precipitation of this extract with 2-propanol yielded major amounts of galactomannan components, while the supernatant was mainly composed of arabinose-rich constituents. Two fractions were obtained by anion-exchange chromatography. The fraction that eluted with water is an arabinan with (1 → 5) α-L linkages and branching mainly on C-2, accompanied with equal amounts of a low-galactose galactomannan oligosaccharide, and a small proportion of a β-(1 → 4)-galactan. The fraction eluted with an increased ionic strength consists mainly of a similar arabinan, and lower proportions of a high-galactose galactomannan, galactan, and protein. The arabinan moiety in both fractions was characterized by chemical analysis and 1D and 2D NMR spectroscopic techniques. © 2002 Elsevier Science Ltd. All rights reserved. Fil:Navarro, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stortz, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00086215_v337_n3_p255_Navarro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-(1 → 4)-Galactan Arabinan Galactomannan Gleditsia triacanthos Legume seeds NMR Chemical analysis Ionic strength Nuclear magnetic resonance spectroscopy Precipitation (chemical) Seed Room temperature Polysaccharides 2 propanol galactomannan galactose oligosaccharide water anion exchange chromatography article chemical analysis controlled study endosperm extraction nonhuman nuclear magnetic resonance spectroscopy precipitation priority journal room temperature seed morphology supernatant Fabaceae Galactans Magnetic Resonance Spectroscopy Mannans Polysaccharides Seeds Gleditsia Gleditsia triacanthos
spellingShingle	β-(1 → 4)-Galactan Arabinan Galactomannan Gleditsia triacanthos Legume seeds NMR Chemical analysis Ionic strength Nuclear magnetic resonance spectroscopy Precipitation (chemical) Seed Room temperature Polysaccharides 2 propanol galactomannan galactose oligosaccharide water anion exchange chromatography article chemical analysis controlled study endosperm extraction nonhuman nuclear magnetic resonance spectroscopy precipitation priority journal room temperature seed morphology supernatant Fabaceae Galactans Magnetic Resonance Spectroscopy Mannans Polysaccharides Seeds Gleditsia Gleditsia triacanthos Navarro, D.A. Cerezo, A.S. Stortz, C.A. NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos
topic_facet	β-(1 → 4)-Galactan Arabinan Galactomannan Gleditsia triacanthos Legume seeds NMR Chemical analysis Ionic strength Nuclear magnetic resonance spectroscopy Precipitation (chemical) Seed Room temperature Polysaccharides 2 propanol galactomannan galactose oligosaccharide water anion exchange chromatography article chemical analysis controlled study endosperm extraction nonhuman nuclear magnetic resonance spectroscopy precipitation priority journal room temperature seed morphology supernatant Fabaceae Galactans Magnetic Resonance Spectroscopy Mannans Polysaccharides Seeds Gleditsia Gleditsia triacanthos
description	Exhaustive extraction of the endosperm from the seed of Gleditsia triacanthos using water at room temperature and 50°C left a residue, which was further extracted at 95°C. Precipitation of this extract with 2-propanol yielded major amounts of galactomannan components, while the supernatant was mainly composed of arabinose-rich constituents. Two fractions were obtained by anion-exchange chromatography. The fraction that eluted with water is an arabinan with (1 → 5) α-L linkages and branching mainly on C-2, accompanied with equal amounts of a low-galactose galactomannan oligosaccharide, and a small proportion of a β-(1 → 4)-galactan. The fraction eluted with an increased ionic strength consists mainly of a similar arabinan, and lower proportions of a high-galactose galactomannan, galactan, and protein. The arabinan moiety in both fractions was characterized by chemical analysis and 1D and 2D NMR spectroscopic techniques. © 2002 Elsevier Science Ltd. All rights reserved.
format	JOUR
author	Navarro, D.A. Cerezo, A.S. Stortz, C.A.
author_facet	Navarro, D.A. Cerezo, A.S. Stortz, C.A.
author_sort	Navarro, D.A.
title	NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos
title_short	NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos
title_full	NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos
title_fullStr	NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos
title_full_unstemmed	NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos
title_sort	nmr spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of gleditsia triacanthos
url	http://hdl.handle.net/20.500.12110/paper_00086215_v337_n3_p255_Navarro
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NMR spectroscopy and chemical studies of an arabinan-rich system from the endosperm of the seed of Gleditsia triacanthos

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