Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata
Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (UV-MALDI-TOF-MS) was applied to sulfated xylo-mannan fractions from Nothogenia fastigiata in order to determine their molecular weights and distribution profiles. The number-average molecular weight calculated...
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todo:paper_00086215_v329_n1_p157_ErraBalsells2023-10-03T14:06:56Z Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata Erra-Balsells, R. Kolender, A.A. Matulewicz, M.C. Nonami, H. Cerezo, A.S. Sulfated polysaccharides Sulfated xylo-mannans UV-MALDI-TOF-MS beta carboline gentisic acid harman isoquinoline derivative mannan sulfur derivative article carbohydrate analysis chemical analysis desorption ionization laser mass spectrometry molecular interaction molecular weight nonhuman priority journal seaweed time ultraviolet radiation Hydroxybenzoic Acids Mannans Molecular Weight Seaweed Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Support, Non-U.S. Gov't Ultraviolet Rays Xylose algae Nothogenia fastigiata Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (UV-MALDI-TOF-MS) was applied to sulfated xylo-mannan fractions from Nothogenia fastigiata in order to determine their molecular weights and distribution profiles. The number-average molecular weight calculated from the spectra was similar to that determined by chemical end-group analysis for the lower molecular weight fractions. For the other fractions, the number-average molecular weight was lower than that chemically determined; the increased difference may be attributed to higher desorption difficulties and, consequently, mass-dependent discrimination. A reconstructed spectrum, using the peaks obtained from all the fractions, suggested an unimodal distribution. The best results were obtained by using 2,5-dihydroxybenzoic acid as matrix doped with 1-hydroxyisoquinoline and with harmane and nor-harmane. (C) 2000 Elsevier Science Ltd. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kolender, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00086215_v329_n1_p157_ErraBalsells |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Sulfated polysaccharides Sulfated xylo-mannans UV-MALDI-TOF-MS beta carboline gentisic acid harman isoquinoline derivative mannan sulfur derivative article carbohydrate analysis chemical analysis desorption ionization laser mass spectrometry molecular interaction molecular weight nonhuman priority journal seaweed time ultraviolet radiation Hydroxybenzoic Acids Mannans Molecular Weight Seaweed Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Support, Non-U.S. Gov't Ultraviolet Rays Xylose algae Nothogenia fastigiata |
spellingShingle |
Sulfated polysaccharides Sulfated xylo-mannans UV-MALDI-TOF-MS beta carboline gentisic acid harman isoquinoline derivative mannan sulfur derivative article carbohydrate analysis chemical analysis desorption ionization laser mass spectrometry molecular interaction molecular weight nonhuman priority journal seaweed time ultraviolet radiation Hydroxybenzoic Acids Mannans Molecular Weight Seaweed Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Support, Non-U.S. Gov't Ultraviolet Rays Xylose algae Nothogenia fastigiata Erra-Balsells, R. Kolender, A.A. Matulewicz, M.C. Nonami, H. Cerezo, A.S. Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata |
topic_facet |
Sulfated polysaccharides Sulfated xylo-mannans UV-MALDI-TOF-MS beta carboline gentisic acid harman isoquinoline derivative mannan sulfur derivative article carbohydrate analysis chemical analysis desorption ionization laser mass spectrometry molecular interaction molecular weight nonhuman priority journal seaweed time ultraviolet radiation Hydroxybenzoic Acids Mannans Molecular Weight Seaweed Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfates Support, Non-U.S. Gov't Ultraviolet Rays Xylose algae Nothogenia fastigiata |
description |
Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (UV-MALDI-TOF-MS) was applied to sulfated xylo-mannan fractions from Nothogenia fastigiata in order to determine their molecular weights and distribution profiles. The number-average molecular weight calculated from the spectra was similar to that determined by chemical end-group analysis for the lower molecular weight fractions. For the other fractions, the number-average molecular weight was lower than that chemically determined; the increased difference may be attributed to higher desorption difficulties and, consequently, mass-dependent discrimination. A reconstructed spectrum, using the peaks obtained from all the fractions, suggested an unimodal distribution. The best results were obtained by using 2,5-dihydroxybenzoic acid as matrix doped with 1-hydroxyisoquinoline and with harmane and nor-harmane. (C) 2000 Elsevier Science Ltd. |
format |
JOUR |
author |
Erra-Balsells, R. Kolender, A.A. Matulewicz, M.C. Nonami, H. Cerezo, A.S. |
author_facet |
Erra-Balsells, R. Kolender, A.A. Matulewicz, M.C. Nonami, H. Cerezo, A.S. |
author_sort |
Erra-Balsells, R. |
title |
Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata |
title_short |
Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata |
title_full |
Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata |
title_fullStr |
Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata |
title_full_unstemmed |
Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata |
title_sort |
matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed nothogenia fastigiata |
url |
http://hdl.handle.net/20.500.12110/paper_00086215_v329_n1_p157_ErraBalsells |
work_keys_str_mv |
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