Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes
A study of nucleotides and nucleotide sugars in mature oocytes of Bufo arenarum has been performed. Two samples of oocytes from ovulations of ten females each were analyzed by ion-exchange chromatography and the following nucleotides and nucleotide sugars found2 2 The following abbreviations are use...
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todo:paper_00086215_v26_n2_p365_FernandezdeRecondo2023-10-03T14:06:42Z Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes Fernández de Recondo, M.E. Maggesse, M.C. Guerrero, G. C. Spaizman, R. Recondo, E.F. carbon nucleoside diphosphate sugar nucleotide animal article embryology female fertilization ion exchange chromatography oocyte ovary paper chromatography paper electrophoresis toad Animal Bufo arenarum Carbon Isotopes Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Female Fertilization Nucleoside Diphosphate Sugars Nucleotides Ovary Ovum A study of nucleotides and nucleotide sugars in mature oocytes of Bufo arenarum has been performed. Two samples of oocytes from ovulations of ten females each were analyzed by ion-exchange chromatography and the following nucleotides and nucleotide sugars found2 2 The following abbreviations are used: ADP, adenosine 5′-pyrophosphate; ATP, adenosine 5′-triphosphate; CTP, cytidine 5′-triphosphate; GDP, guanosine 5′-pyrophosphate; GTP, guanosine 5′-triphosphate; NAD, nicotinamide adenine dinucleotide; NADP, nicotinamide adenine dinucleotide phosphate; UDP, uridine 5′-pyrophosphate; UDPG, uridine 5′-(d-glucosyl pyrophosphate; UDPGA, uridine 5′-(d-glucosyluronic acid pyrophosphate); UDPGalNAc, uridine 5′-(2-acetamido-2-deoxy-d-galactosyl pyrophosphate), UDPGNAc, uridine 5′-(2-acetamido-2-deoxy-d-glucosyl pyrophosphate), and UTP, uridine 5′-triphosphate.: NAD, UDPGNAc, UDPGalNAc, UDPG, UDP, NADP, CTP, UTP, UDPGA, ADP, GDP, GTP, and ATP. The nucleotides and sugar-nucleotides were identified on the basis of chemical, enzymic, and chromatographic analysis. To minimize the decomposition of unstable substances, the extraction was performed with trichloroacetic acid in the cold, the contact of the sample with the acid was minimal and ammonium chloride was used for the elution from the Dowex 1-resin. In order to detect compounds present in minute amounts, very large samples were used (60,000 to 120,000 oocytes). A nucleotide of complex structure, which binds weakly to the column, was detected in the two samples analyzed. © 1973. Fil:Guerrero, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Recondo, E.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00086215_v26_n2_p365_FernandezdeRecondo |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
carbon nucleoside diphosphate sugar nucleotide animal article embryology female fertilization ion exchange chromatography oocyte ovary paper chromatography paper electrophoresis toad Animal Bufo arenarum Carbon Isotopes Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Female Fertilization Nucleoside Diphosphate Sugars Nucleotides Ovary Ovum |
| spellingShingle |
carbon nucleoside diphosphate sugar nucleotide animal article embryology female fertilization ion exchange chromatography oocyte ovary paper chromatography paper electrophoresis toad Animal Bufo arenarum Carbon Isotopes Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Female Fertilization Nucleoside Diphosphate Sugars Nucleotides Ovary Ovum Fernández de Recondo, M.E. Maggesse, M.C. Guerrero, G. C. Spaizman, R. Recondo, E.F. Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes |
| topic_facet |
carbon nucleoside diphosphate sugar nucleotide animal article embryology female fertilization ion exchange chromatography oocyte ovary paper chromatography paper electrophoresis toad Animal Bufo arenarum Carbon Isotopes Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Female Fertilization Nucleoside Diphosphate Sugars Nucleotides Ovary Ovum |
| description |
A study of nucleotides and nucleotide sugars in mature oocytes of Bufo arenarum has been performed. Two samples of oocytes from ovulations of ten females each were analyzed by ion-exchange chromatography and the following nucleotides and nucleotide sugars found2 2 The following abbreviations are used: ADP, adenosine 5′-pyrophosphate; ATP, adenosine 5′-triphosphate; CTP, cytidine 5′-triphosphate; GDP, guanosine 5′-pyrophosphate; GTP, guanosine 5′-triphosphate; NAD, nicotinamide adenine dinucleotide; NADP, nicotinamide adenine dinucleotide phosphate; UDP, uridine 5′-pyrophosphate; UDPG, uridine 5′-(d-glucosyl pyrophosphate; UDPGA, uridine 5′-(d-glucosyluronic acid pyrophosphate); UDPGalNAc, uridine 5′-(2-acetamido-2-deoxy-d-galactosyl pyrophosphate), UDPGNAc, uridine 5′-(2-acetamido-2-deoxy-d-glucosyl pyrophosphate), and UTP, uridine 5′-triphosphate.: NAD, UDPGNAc, UDPGalNAc, UDPG, UDP, NADP, CTP, UTP, UDPGA, ADP, GDP, GTP, and ATP. The nucleotides and sugar-nucleotides were identified on the basis of chemical, enzymic, and chromatographic analysis. To minimize the decomposition of unstable substances, the extraction was performed with trichloroacetic acid in the cold, the contact of the sample with the acid was minimal and ammonium chloride was used for the elution from the Dowex 1-resin. In order to detect compounds present in minute amounts, very large samples were used (60,000 to 120,000 oocytes). A nucleotide of complex structure, which binds weakly to the column, was detected in the two samples analyzed. © 1973. |
| format |
JOUR |
| author |
Fernández de Recondo, M.E. Maggesse, M.C. Guerrero, G. C. Spaizman, R. Recondo, E.F. |
| author_facet |
Fernández de Recondo, M.E. Maggesse, M.C. Guerrero, G. C. Spaizman, R. Recondo, E.F. |
| author_sort |
Fernández de Recondo, M.E. |
| title |
Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes |
| title_short |
Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes |
| title_full |
Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes |
| title_fullStr |
Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes |
| title_full_unstemmed |
Nucleotides and nucleotide sugars in early development of bufo arenarum. Part I. mature oocytes |
| title_sort |
nucleotides and nucleotide sugars in early development of bufo arenarum. part i. mature oocytes |
| url |
http://hdl.handle.net/20.500.12110/paper_00086215_v26_n2_p365_FernandezdeRecondo |
| work_keys_str_mv |
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1807314941191913472 |