Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification

The role of adrenocortical steroids in distal nephron acidification was studied in rats by measuring urine minus blood PCO2 differences (U-B PCO2) in control, sham-operated, and adrenalectomized (ADX) animals. Operations were performed 48 h before experiments. During the experiments, all rats receiv...

Descripción completa

Detalles Bibliográficos
Autores principales: Damasco, M.C., Ansaldo, M., Malnic, G.
Formato: JOUR
Materias:
18 hydroxycorticosterone
aldosterone
bicarbonate
corticosterone
adrenalectomy
animal cell
animal experiment
blood gas
controlled study
kidney function
male
nonhuman
priority journal
rat
urine acidification
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00084212_v67_n6_p607_Damasco
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00084212_v67_n6_p607_Damasco
record_format dspace
spelling todo:paper_00084212_v67_n6_p607_Damasco2023-10-03T14:06:04Z Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification Damasco, M.C. Ansaldo, M. Malnic, G. 18 hydroxycorticosterone aldosterone bicarbonate corticosterone adrenalectomy animal cell animal experiment blood gas controlled study kidney function male nonhuman priority journal rat urine acidification The role of adrenocortical steroids in distal nephron acidification was studied in rats by measuring urine minus blood PCO2 differences (U-B PCO2) in control, sham-operated, and adrenalectomized (ADX) animals. Operations were performed 48 h before experiments. During the experiments, all rats received an infusion of 0.35-0.60 M NaHCO3, leading to urine bicarbonate concentrations in the order of 100-200 mM. Adrenalectomized rats had significantly decreased U-B PCO2 (11.9 ± 1.99 mmHg; 1 mmHg = 133.3 Pa) with respect to sham-operated rats (39.9 ± 1.26 mmHg). In another series, ADX rats received supplements of the adrenal steroids corticosterone, aldosterone, and 18-hydroxycorticosterone 100 min before the experiment. U-B PCO2 increased after hormone administration: corticosterone, 30.0 ± 2.13 mmHg; aldosterone, 26.6 ± 1.74 mmHg; 18-hydroxycorticosterone, 29.0 ± 1.60 mmHg; but none restored these values to normal. Combinations of two hormones were also used; only aldosterone + corticosterone restored U-B PCO2 to normal: 39.0 ± 1.66 mmHg. Renal phosphate excretion (but not urine phosphate levels) decreased significantly in ADX as compared with sham-operated rats. Extracellular volume was not significantly affected in ADX rats, which received ad libitum 0.9% NaCl for drinking. It is concluded that distal tubular acidification, as evaluated by U-B PCO2, is dependent on cortical steroids. Fil:Ansaldo, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00084212_v67_n6_p607_Damasco
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 18 hydroxycorticosterone
aldosterone
bicarbonate
corticosterone
adrenalectomy
animal cell
animal experiment
blood gas
controlled study
kidney function
male
nonhuman
priority journal
rat
urine acidification
spellingShingle 18 hydroxycorticosterone
aldosterone
bicarbonate
corticosterone
adrenalectomy
animal cell
animal experiment
blood gas
controlled study
kidney function
male
nonhuman
priority journal
rat
urine acidification
Damasco, M.C.
Ansaldo, M.
Malnic, G.
Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
topic_facet 18 hydroxycorticosterone
aldosterone
bicarbonate
corticosterone
adrenalectomy
animal cell
animal experiment
blood gas
controlled study
kidney function
male
nonhuman
priority journal
rat
urine acidification
description The role of adrenocortical steroids in distal nephron acidification was studied in rats by measuring urine minus blood PCO2 differences (U-B PCO2) in control, sham-operated, and adrenalectomized (ADX) animals. Operations were performed 48 h before experiments. During the experiments, all rats received an infusion of 0.35-0.60 M NaHCO3, leading to urine bicarbonate concentrations in the order of 100-200 mM. Adrenalectomized rats had significantly decreased U-B PCO2 (11.9 ± 1.99 mmHg; 1 mmHg = 133.3 Pa) with respect to sham-operated rats (39.9 ± 1.26 mmHg). In another series, ADX rats received supplements of the adrenal steroids corticosterone, aldosterone, and 18-hydroxycorticosterone 100 min before the experiment. U-B PCO2 increased after hormone administration: corticosterone, 30.0 ± 2.13 mmHg; aldosterone, 26.6 ± 1.74 mmHg; 18-hydroxycorticosterone, 29.0 ± 1.60 mmHg; but none restored these values to normal. Combinations of two hormones were also used; only aldosterone + corticosterone restored U-B PCO2 to normal: 39.0 ± 1.66 mmHg. Renal phosphate excretion (but not urine phosphate levels) decreased significantly in ADX as compared with sham-operated rats. Extracellular volume was not significantly affected in ADX rats, which received ad libitum 0.9% NaCl for drinking. It is concluded that distal tubular acidification, as evaluated by U-B PCO2, is dependent on cortical steroids.
format JOUR
author Damasco, M.C.
Ansaldo, M.
Malnic, G.
author_facet Damasco, M.C.
Ansaldo, M.
Malnic, G.
author_sort Damasco, M.C.
title Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_short Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_full Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_fullStr Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_full_unstemmed Effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
title_sort effects of adrenalectomy and acute replacement by corticosteroids on distal acidification
url http://hdl.handle.net/20.500.12110/paper_00084212_v67_n6_p607_Damasco
work_keys_str_mv AT damascomc effectsofadrenalectomyandacutereplacementbycorticosteroidsondistalacidification
AT ansaldom effectsofadrenalectomyandacutereplacementbycorticosteroidsondistalacidification
AT malnicg effectsofadrenalectomyandacutereplacementbycorticosteroidsondistalacidification
_version_ 1807322805402861568

Ejemplares similares