δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters

δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system i...

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Autores principales:	Bermúdez Moretti, M., Correa García, S., Perotti, C., Batlle, A., Casas, A.
Formato:	JOUR
Materias:	BETA transporters Membrane transport Photodynamic therapy δ-aminolevulinic acid aminolevulinic acid carrier protein animal cell animal model article breast adenocarcinoma cancer cell culture cell membrane transport controlled study diffusion drug transport drug uptake mouse nonhuman priority journal transport kinetics Adenocarcinoma Aminolevulinic Acid Animals Biological Transport, Active Mammary Neoplasms, Experimental Mice Tumor Cells, Cultured
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00070920_v87_n4_p471_BermudezMoretti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00070920_v87_n4_p471_BermudezMoretti
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spelling	todo:paper_00070920_v87_n4_p471_BermudezMoretti2023-10-03T14:05:27Z δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters Bermúdez Moretti, M. Correa García, S. Perotti, C. Batlle, A. Casas, A. BETA transporters Membrane transport Photodynamic therapy δ-aminolevulinic acid aminolevulinic acid carrier protein animal cell animal model article breast adenocarcinoma cancer cell culture cell membrane transport controlled study diffusion drug transport drug uptake mouse nonhuman priority journal transport kinetics Adenocarcinoma Aminolevulinic Acid Animals Biological Transport, Active Mammary Neoplasms, Experimental Mice Tumor Cells, Cultured δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using 14C-δ-aminolevulinic acid, to finally improve δ-aminolevulinic acid incorporation in mammalian cells. Our results showed that δ aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in δ-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2. © 2002 Cancer Research UK. Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00070920_v87_n4_p471_BermudezMoretti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	BETA transporters Membrane transport Photodynamic therapy δ-aminolevulinic acid aminolevulinic acid carrier protein animal cell animal model article breast adenocarcinoma cancer cell culture cell membrane transport controlled study diffusion drug transport drug uptake mouse nonhuman priority journal transport kinetics Adenocarcinoma Aminolevulinic Acid Animals Biological Transport, Active Mammary Neoplasms, Experimental Mice Tumor Cells, Cultured
spellingShingle	BETA transporters Membrane transport Photodynamic therapy δ-aminolevulinic acid aminolevulinic acid carrier protein animal cell animal model article breast adenocarcinoma cancer cell culture cell membrane transport controlled study diffusion drug transport drug uptake mouse nonhuman priority journal transport kinetics Adenocarcinoma Aminolevulinic Acid Animals Biological Transport, Active Mammary Neoplasms, Experimental Mice Tumor Cells, Cultured Bermúdez Moretti, M. Correa García, S. Perotti, C. Batlle, A. Casas, A. δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
topic_facet	BETA transporters Membrane transport Photodynamic therapy δ-aminolevulinic acid aminolevulinic acid carrier protein animal cell animal model article breast adenocarcinoma cancer cell culture cell membrane transport controlled study diffusion drug transport drug uptake mouse nonhuman priority journal transport kinetics Adenocarcinoma Aminolevulinic Acid Animals Biological Transport, Active Mammary Neoplasms, Experimental Mice Tumor Cells, Cultured
description	δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using 14C-δ-aminolevulinic acid, to finally improve δ-aminolevulinic acid incorporation in mammalian cells. Our results showed that δ aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in δ-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2. © 2002 Cancer Research UK.
format	JOUR
author	Bermúdez Moretti, M. Correa García, S. Perotti, C. Batlle, A. Casas, A.
author_facet	Bermúdez Moretti, M. Correa García, S. Perotti, C. Batlle, A. Casas, A.
author_sort	Bermúdez Moretti, M.
title	δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_short	δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_full	δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_fullStr	δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_full_unstemmed	δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
title_sort	δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
url	http://hdl.handle.net/20.500.12110/paper_00070920_v87_n4_p471_BermudezMoretti
work_keys_str_mv	AT bermudezmorettim daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters AT correagarcias daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters AT perottic daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters AT batllea daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters AT casasa daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters
_version_	1807318375135707136

δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters

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