Tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ALA in different vehicles

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The use of 5-aminolaevulinic acid (ALA) is gaining increasing attention for photosensitization in photodynamic therapy of superficially localized tumours. The aim of this work was to determine the kinetics of porphyrin generation in tissues after topical application of ALA delivered in different veh...

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Autores principales:	Casas, A., Fukuda, H., Del C Batlle, A.M.
Formato:	JOUR
Materias:	5-Aminolaevulinic acid ALA cream ALA lotion ALA-PDT Topical application aminolevulinic acid drug vehicle photosensitizing agent porphyrin animal experiment animal model animal tissue article cancer therapy controlled study cream feces level kidney kinetics liver lotion male mouse nonhuman photodynamic therapy priority journal skin spleen tissue distribution topical drug administration urine level Adenocarcinoma Administration, Topical Aminolevulinic Acid Animals Kinetics Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Transplantation Photosensitizing Agents Porphyrins Tissue Distribution Vehicles
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00070920_v81_n1_p13_Casas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00070920_v81_n1_p13_Casas
record_format	dspace
spelling	todo:paper_00070920_v81_n1_p13_Casas2023-10-03T14:05:24Z Tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ALA in different vehicles Casas, A. Fukuda, H. Del C Batlle, A.M. 5-Aminolaevulinic acid ALA cream ALA lotion ALA-PDT Topical application aminolevulinic acid drug vehicle photosensitizing agent porphyrin animal experiment animal model animal tissue article cancer therapy controlled study cream feces level kidney kinetics liver lotion male mouse nonhuman photodynamic therapy priority journal skin spleen tissue distribution topical drug administration urine level Adenocarcinoma Administration, Topical Aminolevulinic Acid Animals Kinetics Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Transplantation Photosensitizing Agents Porphyrins Tissue Distribution Vehicles The use of 5-aminolaevulinic acid (ALA) is gaining increasing attention for photosensitization in photodynamic therapy of superficially localized tumours. The aim of this work was to determine the kinetics of porphyrin generation in tissues after topical application of ALA delivered in different vehicles on the skin overlying the tumour and normal skin of mice. Maximal accumulation was found in tumour 3 h after ALA application in both cream and lotion preparations. Normal and overlying tumour skin tissues showed different kinetic patterns, reflecting histological changes when the latter is invaded by tumour cells. Liver, kidney, spleen and blood porphyrins also raised from basal levels, showing that ALA and/or ALA-induced porphyrins reach all tissues after topical application. During the first 24 h of ALA topical application, precursors and porphyrins are excreted by both urine and faeces. ALA lotion applied on the skin overlying the tumour induced higher accumulation of tumoural porphyrins than cream, and lotion applied on normal skin appeared to be the most efficient upon inducing total body porphyrins. This work has demonstrated the great influence of the formulation of ALA vehicle on penetration through the skin. Knowledge of the kinetics of porphyrin generation after different conditions of ALA application is needed for the optimization of diagnosis and phototherapy in human tumours. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Del C Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00070920_v81_n1_p13_Casas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-Aminolaevulinic acid ALA cream ALA lotion ALA-PDT Topical application aminolevulinic acid drug vehicle photosensitizing agent porphyrin animal experiment animal model animal tissue article cancer therapy controlled study cream feces level kidney kinetics liver lotion male mouse nonhuman photodynamic therapy priority journal skin spleen tissue distribution topical drug administration urine level Adenocarcinoma Administration, Topical Aminolevulinic Acid Animals Kinetics Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Transplantation Photosensitizing Agents Porphyrins Tissue Distribution Vehicles
spellingShingle	5-Aminolaevulinic acid ALA cream ALA lotion ALA-PDT Topical application aminolevulinic acid drug vehicle photosensitizing agent porphyrin animal experiment animal model animal tissue article cancer therapy controlled study cream feces level kidney kinetics liver lotion male mouse nonhuman photodynamic therapy priority journal skin spleen tissue distribution topical drug administration urine level Adenocarcinoma Administration, Topical Aminolevulinic Acid Animals Kinetics Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Transplantation Photosensitizing Agents Porphyrins Tissue Distribution Vehicles Casas, A. Fukuda, H. Del C Batlle, A.M. Tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ALA in different vehicles
topic_facet	5-Aminolaevulinic acid ALA cream ALA lotion ALA-PDT Topical application aminolevulinic acid drug vehicle photosensitizing agent porphyrin animal experiment animal model animal tissue article cancer therapy controlled study cream feces level kidney kinetics liver lotion male mouse nonhuman photodynamic therapy priority journal skin spleen tissue distribution topical drug administration urine level Adenocarcinoma Administration, Topical Aminolevulinic Acid Animals Kinetics Male Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Neoplasm Transplantation Photosensitizing Agents Porphyrins Tissue Distribution Vehicles
description	The use of 5-aminolaevulinic acid (ALA) is gaining increasing attention for photosensitization in photodynamic therapy of superficially localized tumours. The aim of this work was to determine the kinetics of porphyrin generation in tissues after topical application of ALA delivered in different vehicles on the skin overlying the tumour and normal skin of mice. Maximal accumulation was found in tumour 3 h after ALA application in both cream and lotion preparations. Normal and overlying tumour skin tissues showed different kinetic patterns, reflecting histological changes when the latter is invaded by tumour cells. Liver, kidney, spleen and blood porphyrins also raised from basal levels, showing that ALA and/or ALA-induced porphyrins reach all tissues after topical application. During the first 24 h of ALA topical application, precursors and porphyrins are excreted by both urine and faeces. ALA lotion applied on the skin overlying the tumour induced higher accumulation of tumoural porphyrins than cream, and lotion applied on normal skin appeared to be the most efficient upon inducing total body porphyrins. This work has demonstrated the great influence of the formulation of ALA vehicle on penetration through the skin. Knowledge of the kinetics of porphyrin generation after different conditions of ALA application is needed for the optimization of diagnosis and phototherapy in human tumours.
format	JOUR
author	Casas, A. Fukuda, H. Del C Batlle, A.M.
author_facet	Casas, A. Fukuda, H. Del C Batlle, A.M.
author_sort	Casas, A.
title	Tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ALA in different vehicles
title_short	Tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ALA in different vehicles
title_full	Tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ALA in different vehicles
title_fullStr	Tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ALA in different vehicles
title_full_unstemmed	Tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ALA in different vehicles
title_sort	tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ala in different vehicles
url	http://hdl.handle.net/20.500.12110/paper_00070920_v81_n1_p13_Casas
work_keys_str_mv	AT casasa tissuedistributionandkineticsofendogenousporphyrinssynthesizedaftertopicalapplicationofalaindifferentvehicles AT fukudah tissuedistributionandkineticsofendogenousporphyrinssynthesizedaftertopicalapplicationofalaindifferentvehicles AT delcbatlleam tissuedistributionandkineticsofendogenousporphyrinssynthesizedaftertopicalapplicationofalaindifferentvehicles
_version_	1807318727333511168

Tissue distribution and kinetics of endogenous porphyrins synthesized after topical application of ALA in different vehicles

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