Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells

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Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, g...

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Autores principales:	Pérez-Pérez, A., Julieta Maymo, Y., Gambino, É., Dueñas, J.L., Goberna, R., Varone, C., Sánchez-Margalet, V.
Formato:	JOUR
Materias:	Leptin Leptin receptor Mechanisms of hormone action Placenta Trophoblast initiation factor 4E leptin mitogen activated protein kinase phosphatidylinositol 3 kinase 2 (2 amino 3 methoxyphenyl)chromone androstane derivative EIF4EBP1 protein, human flavonoid phosphoprotein protein kinase inhibitor signal transducing adaptor protein wortmannin article cell growth controlled study hormone action human human cell priority journal protein binding protein phosphorylation protein synthesis translation initiation trophoblast analysis of variance cell culture cell line dose response drug effect female metabolism phosphorylation physiology placenta pregnancy signal transduction Western blotting 1-Phosphatidylinositol 3-Kinase Adaptor Proteins, Signal Transducing Analysis of Variance Androstadienes Blotting, Western Cell Line Cells, Cultured Dose-Response Relationship, Drug Eukaryotic Initiation Factor-4E Female Flavonoids Humans Mitogen-Activated Protein Kinases Phosphoproteins Phosphorylation Pregnancy Protein Biosynthesis Protein Kinase Inhibitors Signal Transduction Trophoblasts
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00063363_v81_n5_p826_PerezPerez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00063363_v81_n5_p826_PerezPerez
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spelling	todo:paper_00063363_v81_n5_p826_PerezPerez2023-10-03T14:04:58Z Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells Pérez-Pérez, A. Julieta Maymo, Y. Gambino, É. Dueñas, J.L. Goberna, R. Varone, C. Sánchez-Margalet, V. Leptin Leptin receptor Mechanisms of hormone action Placenta Trophoblast initiation factor 4E leptin mitogen activated protein kinase phosphatidylinositol 3 kinase 2 (2 amino 3 methoxyphenyl)chromone androstane derivative EIF4EBP1 protein, human flavonoid initiation factor 4E leptin mitogen activated protein kinase phosphatidylinositol 3 kinase phosphoprotein protein kinase inhibitor signal transducing adaptor protein wortmannin article cell growth controlled study hormone action human human cell priority journal protein binding protein phosphorylation protein synthesis translation initiation trophoblast analysis of variance cell culture cell line dose response drug effect female metabolism phosphorylation physiology placenta pregnancy protein synthesis signal transduction Western blotting 1-Phosphatidylinositol 3-Kinase Adaptor Proteins, Signal Transducing Analysis of Variance Androstadienes Blotting, Western Cell Line Cells, Cultured Dose-Response Relationship, Drug Eukaryotic Initiation Factor-4E Female Flavonoids Humans Leptin Mitogen-Activated Protein Kinases Phosphoproteins Phosphorylation Placenta Pregnancy Protein Biosynthesis Protein Kinase Inhibitors Signal Transduction Trophoblasts Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc. Fil:Julieta Maymo, Y. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gambino, É. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Varone, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00063363_v81_n5_p826_PerezPerez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Leptin Leptin receptor Mechanisms of hormone action Placenta Trophoblast initiation factor 4E leptin mitogen activated protein kinase phosphatidylinositol 3 kinase 2 (2 amino 3 methoxyphenyl)chromone androstane derivative EIF4EBP1 protein, human flavonoid initiation factor 4E leptin mitogen activated protein kinase phosphatidylinositol 3 kinase phosphoprotein protein kinase inhibitor signal transducing adaptor protein wortmannin article cell growth controlled study hormone action human human cell priority journal protein binding protein phosphorylation protein synthesis translation initiation trophoblast analysis of variance cell culture cell line dose response drug effect female metabolism phosphorylation physiology placenta pregnancy protein synthesis signal transduction Western blotting 1-Phosphatidylinositol 3-Kinase Adaptor Proteins, Signal Transducing Analysis of Variance Androstadienes Blotting, Western Cell Line Cells, Cultured Dose-Response Relationship, Drug Eukaryotic Initiation Factor-4E Female Flavonoids Humans Leptin Mitogen-Activated Protein Kinases Phosphoproteins Phosphorylation Placenta Pregnancy Protein Biosynthesis Protein Kinase Inhibitors Signal Transduction Trophoblasts
spellingShingle	Leptin Leptin receptor Mechanisms of hormone action Placenta Trophoblast initiation factor 4E leptin mitogen activated protein kinase phosphatidylinositol 3 kinase 2 (2 amino 3 methoxyphenyl)chromone androstane derivative EIF4EBP1 protein, human flavonoid initiation factor 4E leptin mitogen activated protein kinase phosphatidylinositol 3 kinase phosphoprotein protein kinase inhibitor signal transducing adaptor protein wortmannin article cell growth controlled study hormone action human human cell priority journal protein binding protein phosphorylation protein synthesis translation initiation trophoblast analysis of variance cell culture cell line dose response drug effect female metabolism phosphorylation physiology placenta pregnancy protein synthesis signal transduction Western blotting 1-Phosphatidylinositol 3-Kinase Adaptor Proteins, Signal Transducing Analysis of Variance Androstadienes Blotting, Western Cell Line Cells, Cultured Dose-Response Relationship, Drug Eukaryotic Initiation Factor-4E Female Flavonoids Humans Leptin Mitogen-Activated Protein Kinases Phosphoproteins Phosphorylation Placenta Pregnancy Protein Biosynthesis Protein Kinase Inhibitors Signal Transduction Trophoblasts Pérez-Pérez, A. Julieta Maymo, Y. Gambino, É. Dueñas, J.L. Goberna, R. Varone, C. Sánchez-Margalet, V. Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
topic_facet	Leptin Leptin receptor Mechanisms of hormone action Placenta Trophoblast initiation factor 4E leptin mitogen activated protein kinase phosphatidylinositol 3 kinase 2 (2 amino 3 methoxyphenyl)chromone androstane derivative EIF4EBP1 protein, human flavonoid initiation factor 4E leptin mitogen activated protein kinase phosphatidylinositol 3 kinase phosphoprotein protein kinase inhibitor signal transducing adaptor protein wortmannin article cell growth controlled study hormone action human human cell priority journal protein binding protein phosphorylation protein synthesis translation initiation trophoblast analysis of variance cell culture cell line dose response drug effect female metabolism phosphorylation physiology placenta pregnancy protein synthesis signal transduction Western blotting 1-Phosphatidylinositol 3-Kinase Adaptor Proteins, Signal Transducing Analysis of Variance Androstadienes Blotting, Western Cell Line Cells, Cultured Dose-Response Relationship, Drug Eukaryotic Initiation Factor-4E Female Flavonoids Humans Leptin Mitogen-Activated Protein Kinases Phosphoproteins Phosphorylation Placenta Pregnancy Protein Biosynthesis Protein Kinase Inhibitors Signal Transduction Trophoblasts
description	Leptin was originally considered as an adipocyte-derived signaling molecule for the central control of metabolism. However, pleiotropic effects of leptin have been identified in reproduction and pregnancy, particularly in placenta, where it may work as an autocrine hormone, mediating angiogenesis, growth, and immunomodulation. Leptin receptor (LEPR, also known as Ob-R) shows sequence homology to members of the class I cytokine receptor (gp130) superfamily. In fact, leptin may function as a proinflammatory cytokine. We have previously found that leptin is a trophic and mitogenic factor for trophoblastic cells. In order to further investigate the mechanism by which leptin stimulates cell growth in JEG-3 cells and trophoblastic cells, we studied the phosphorylation state of different proteins of the initiation stage of translation and the total protein synthesis by [3H]leucine incorporation in JEG-3 cells. We have found that leptin dose-dependently stimulates the phosphorylation and activation of the translation initiation factor EIF4E as well as the phosphorylation of the EIF4E binding protein EIF4EBP1 (PHAS-I), which releases EIF4E to form active complexes. Moreover, leptin dose-dependently stimulates protein synthesis, and this effect can be partially prevented by blocking mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3 kinase (PIK3) pathways. In conclusion, leptin stimulates protein synthesis, at least in part activating the translation machinery, via the activation of MAPK and PIK3 pathways. © 2009 by the Society for the Study of Reproduction, Inc.
format	JOUR
author	Pérez-Pérez, A. Julieta Maymo, Y. Gambino, É. Dueñas, J.L. Goberna, R. Varone, C. Sánchez-Margalet, V.
author_facet	Pérez-Pérez, A. Julieta Maymo, Y. Gambino, É. Dueñas, J.L. Goberna, R. Varone, C. Sánchez-Margalet, V.
author_sort	Pérez-Pérez, A.
title	Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title_short	Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title_full	Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title_fullStr	Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title_full_unstemmed	Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
title_sort	leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells
url	http://hdl.handle.net/20.500.12110/paper_00063363_v81_n5_p826_PerezPerez
work_keys_str_mv	AT perezpereza leptinstimulatesproteinsynthesisactivatingtranslationmachineryinhumantrophoblasticcells AT julietamaymoy leptinstimulatesproteinsynthesisactivatingtranslationmachineryinhumantrophoblasticcells AT gambinoe leptinstimulatesproteinsynthesisactivatingtranslationmachineryinhumantrophoblasticcells AT duenasjl leptinstimulatesproteinsynthesisactivatingtranslationmachineryinhumantrophoblasticcells AT gobernar leptinstimulatesproteinsynthesisactivatingtranslationmachineryinhumantrophoblasticcells AT varonec leptinstimulatesproteinsynthesisactivatingtranslationmachineryinhumantrophoblasticcells AT sanchezmargaletv leptinstimulatesproteinsynthesisactivatingtranslationmachineryinhumantrophoblasticcells
_version_	1782028559503589376

Leptin stimulates protein synthesis-activating translation machinery in human trophoblastic cells

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