Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability

Numerous studies have demonstrated that sperm capacitation is a bicarbonate-dependent process. In the rat, capacitation has not been studied as much as in other species, mainly because of the difficulties in carrying out functional assays with this animal model. In the present study, we have examine...

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Autores principales: Da Ros, V.G., Munuce, M.J., Cohen, D.J., Marín-Briggiler, C.I., Busso, D., Visconti, P.E., Cuasnicú, P.S.
Formato: JOUR
Materias:
Epididymis
Fertilization
Gamete biology
Sperm capacitation
anion
bicarbonate
bucladesine
cyclic AMP derivative
epididymal protein de
pentoxifylline
phosphodiesterase inhibitor
protein
unclassified drug
animal cell
article
cell fusion
controlled study
egg
exposure
female
gamete
incubation time
male
nonhuman
parameter
priority journal
protein phosphorylation
rat
sperm
spermatozoon
spermatozoon capacitation
Animals
Bicarbonates
Biological Transport
Female
Male
Membrane Glycoproteins
Phosphorylation
Rats
Rats, Sprague-Dawley
Sperm Capacitation
Sperm-Ovum Interactions
Spermatozoa
Tyrosine
Animalia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00063363_v70_n5_p1325_DaRos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00063363_v70_n5_p1325_DaRos
record_format dspace
spelling todo:paper_00063363_v70_n5_p1325_DaRos2023-10-03T14:04:54Z Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability Da Ros, V.G. Munuce, M.J. Cohen, D.J. Marín-Briggiler, C.I. Busso, D. Visconti, P.E. Cuasnicú, P.S. Epididymis Fertilization Gamete biology Sperm capacitation anion bicarbonate bucladesine cyclic AMP derivative epididymal protein de pentoxifylline phosphodiesterase inhibitor protein unclassified drug animal cell article cell fusion controlled study egg exposure female gamete incubation time male nonhuman parameter priority journal protein phosphorylation rat sperm spermatozoon spermatozoon capacitation Animals Bicarbonates Biological Transport Female Male Membrane Glycoproteins Phosphorylation Rats Rats, Sprague-Dawley Sperm Capacitation Sperm-Ovum Interactions Spermatozoa Tyrosine Animalia Numerous studies have demonstrated that sperm capacitation is a bicarbonate-dependent process. In the rat, capacitation has not been studied as much as in other species, mainly because of the difficulties in carrying out functional assays with this animal model. In the present study, we have examined the influence of bicarbonate in the overall rat sperm capacitation process by analyzing involvement of the anion in 1) protein tyrosine phosphorylation, 2) migration of epididymal protein DE (also known as CRISP-1) from the dorsal region to the equatorial segment of the sperm head that occurs during capacitation, and 3) ability of sperm to fuse with the egg. Incubation of sperm under capacitating conditions produced a time-dependent increase in protein tyrosine phosphorylation. This phosphorylation did not occur in the absence of HCO3- and rapidly increased by either exposure of sperm to HCO3- or replacement of the anion by a cAMP analog (dibutyryl-cAMP) and a phosphodiesterase inhibitor (pentoxifylline). The absence of HCO3- also produced a significant decrease in the percentage of cells showing migration of DE to the equatorial segment. This parameter was completely restored by addition of the anion, but dibutyryl-cAMP and pentoxifylline were not sufficient to overcome the decrease in DE migration. Sperm capacitated in the absence of HCO3- were unable to penetrate zona-free eggs independent of the presence of the anion during gamete coincubation. Exposure of these sperm to bicarbonate, or replacement of the anion by dibutyryl-cAMP and pentoxifylline, only partially restored the sperm fusion ability. Altogether, these results indicate that, in addition to its influence on protein tyrosine phosphorylation, bicarbonate is required to support other rat sperm capacitation-associated events, such as migration of DE to the equatorial segment, and expression of the ability of sperm to fuse with the egg. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00063363_v70_n5_p1325_DaRos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Epididymis
Fertilization
Gamete biology
Sperm capacitation
anion
bicarbonate
bucladesine
cyclic AMP derivative
epididymal protein de
pentoxifylline
phosphodiesterase inhibitor
protein
unclassified drug
animal cell
article
cell fusion
controlled study
egg
exposure
female
gamete
incubation time
male
nonhuman
parameter
priority journal
protein phosphorylation
rat
sperm
spermatozoon
spermatozoon capacitation
Animals
Bicarbonates
Biological Transport
Female
Male
Membrane Glycoproteins
Phosphorylation
Rats
Rats, Sprague-Dawley
Sperm Capacitation
Sperm-Ovum Interactions
Spermatozoa
Tyrosine
Animalia
spellingShingle Epididymis
Fertilization
Gamete biology
Sperm capacitation
anion
bicarbonate
bucladesine
cyclic AMP derivative
epididymal protein de
pentoxifylline
phosphodiesterase inhibitor
protein
unclassified drug
animal cell
article
cell fusion
controlled study
egg
exposure
female
gamete
incubation time
male
nonhuman
parameter
priority journal
protein phosphorylation
rat
sperm
spermatozoon
spermatozoon capacitation
Animals
Bicarbonates
Biological Transport
Female
Male
Membrane Glycoproteins
Phosphorylation
Rats
Rats, Sprague-Dawley
Sperm Capacitation
Sperm-Ovum Interactions
Spermatozoa
Tyrosine
Animalia
Da Ros, V.G.
Munuce, M.J.
Cohen, D.J.
Marín-Briggiler, C.I.
Busso, D.
Visconti, P.E.
Cuasnicú, P.S.
Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability
topic_facet Epididymis
Fertilization
Gamete biology
Sperm capacitation
anion
bicarbonate
bucladesine
cyclic AMP derivative
epididymal protein de
pentoxifylline
phosphodiesterase inhibitor
protein
unclassified drug
animal cell
article
cell fusion
controlled study
egg
exposure
female
gamete
incubation time
male
nonhuman
parameter
priority journal
protein phosphorylation
rat
sperm
spermatozoon
spermatozoon capacitation
Animals
Bicarbonates
Biological Transport
Female
Male
Membrane Glycoproteins
Phosphorylation
Rats
Rats, Sprague-Dawley
Sperm Capacitation
Sperm-Ovum Interactions
Spermatozoa
Tyrosine
Animalia
description Numerous studies have demonstrated that sperm capacitation is a bicarbonate-dependent process. In the rat, capacitation has not been studied as much as in other species, mainly because of the difficulties in carrying out functional assays with this animal model. In the present study, we have examined the influence of bicarbonate in the overall rat sperm capacitation process by analyzing involvement of the anion in 1) protein tyrosine phosphorylation, 2) migration of epididymal protein DE (also known as CRISP-1) from the dorsal region to the equatorial segment of the sperm head that occurs during capacitation, and 3) ability of sperm to fuse with the egg. Incubation of sperm under capacitating conditions produced a time-dependent increase in protein tyrosine phosphorylation. This phosphorylation did not occur in the absence of HCO3- and rapidly increased by either exposure of sperm to HCO3- or replacement of the anion by a cAMP analog (dibutyryl-cAMP) and a phosphodiesterase inhibitor (pentoxifylline). The absence of HCO3- also produced a significant decrease in the percentage of cells showing migration of DE to the equatorial segment. This parameter was completely restored by addition of the anion, but dibutyryl-cAMP and pentoxifylline were not sufficient to overcome the decrease in DE migration. Sperm capacitated in the absence of HCO3- were unable to penetrate zona-free eggs independent of the presence of the anion during gamete coincubation. Exposure of these sperm to bicarbonate, or replacement of the anion by dibutyryl-cAMP and pentoxifylline, only partially restored the sperm fusion ability. Altogether, these results indicate that, in addition to its influence on protein tyrosine phosphorylation, bicarbonate is required to support other rat sperm capacitation-associated events, such as migration of DE to the equatorial segment, and expression of the ability of sperm to fuse with the egg.
format JOUR
author Da Ros, V.G.
Munuce, M.J.
Cohen, D.J.
Marín-Briggiler, C.I.
Busso, D.
Visconti, P.E.
Cuasnicú, P.S.
author_facet Da Ros, V.G.
Munuce, M.J.
Cohen, D.J.
Marín-Briggiler, C.I.
Busso, D.
Visconti, P.E.
Cuasnicú, P.S.
author_sort Da Ros, V.G.
title Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability
title_short Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability
title_full Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability
title_fullStr Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability
title_full_unstemmed Bicarbonate Is Required for Migration of Sperm Epididymal Protein DE (CRISP-1) to the Equatorial Segment and Expression of Rat Sperm Fusion Ability
title_sort bicarbonate is required for migration of sperm epididymal protein de (crisp-1) to the equatorial segment and expression of rat sperm fusion ability
url http://hdl.handle.net/20.500.12110/paper_00063363_v70_n5_p1325_DaRos
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