Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation

We evaluated the effects of transforming growth factor β1 (TGFβ1), alone or in combination with FSH and estradiol, on DNA synthesis in primary cultures of immature rat granulosa cells. 3H-Thymidine incorporation was significantly stimulated by TGFβ1 (5.6-fold). This effect was enhanced by FSH (20 ng...

Descripción completa

Detalles Bibliográficos
Autores principales:	Saragüeta, P.E., Lanuza, G.M., Barañao, J.L.
Formato:	JOUR
Materias:	Estradiol Follicle-stimulating hormone Granulosa cells Growth factors estradiol fibronectin follitropin thymidine transforming growth factor beta1 arginyl-glycyl-aspartic acid arginylglycylaspartic acid DNA oligopeptide peptide fragment Tgfb1 protein, rat transforming growth factor beta animal cell article autocrine effect cell proliferation controlled study DNA synthesis female granulosa cell hormone action nonhuman priority journal protein binding protein domain rat animal binding site biosynthesis cell culture cell division chemistry culture medium cytology drug antagonism drug effect drug interaction metabolism physiology Sprague Dawley rat Animalia Animals Binding Sites Cell Division Cells, Cultured Culture Media, Conditioned Drug Interactions Female Fibronectins Follicle Stimulating Hormone Granulosa Cells Oligopeptides Peptide Fragments Rats Rats, Sprague-Dawley Transforming Growth Factor beta Transforming Growth Factor beta1
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00063363_v66_n6_p1862_Saragueta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00063363_v66_n6_p1862_Saragueta
record_format	dspace
spelling	todo:paper_00063363_v66_n6_p1862_Saragueta2023-10-03T14:04:51Z Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation Saragüeta, P.E. Lanuza, G.M. Barañao, J.L. Estradiol Follicle-stimulating hormone Granulosa cells Growth factors estradiol fibronectin follitropin thymidine transforming growth factor beta1 arginyl-glycyl-aspartic acid arginylglycylaspartic acid DNA estradiol fibronectin follitropin oligopeptide peptide fragment Tgfb1 protein, rat transforming growth factor beta transforming growth factor beta1 animal cell article autocrine effect cell proliferation controlled study DNA synthesis female granulosa cell hormone action nonhuman priority journal protein binding protein domain rat animal binding site biosynthesis cell culture cell division chemistry culture medium cytology drug antagonism drug effect drug interaction metabolism physiology Sprague Dawley rat Animalia Animals Binding Sites Cell Division Cells, Cultured Culture Media, Conditioned DNA Drug Interactions Estradiol Female Fibronectins Follicle Stimulating Hormone Granulosa Cells Oligopeptides Peptide Fragments Rats Rats, Sprague-Dawley Transforming Growth Factor beta Transforming Growth Factor beta1 We evaluated the effects of transforming growth factor β1 (TGFβ1), alone or in combination with FSH and estradiol, on DNA synthesis in primary cultures of immature rat granulosa cells. 3H-Thymidine incorporation was significantly stimulated by TGFβ1 (5.6-fold). This effect was enhanced by FSH (20 ng/ml, 27.7-fold) or estradiol (100 ng/ml, 13.4-fold) or by a combination of both hormones (59.2-fold). Measurement of TGFβ bioactivity showed the presence of significant amounts of TGFβ in conditioned medium from granulosa cell cultures, and most of the activity was present in the latent form. FSH alone or in combination with estradiol produced a marked suppression of the production of latent and active TGFβ. Activated conditioned medium from control cultures of granulosa cell elicited a 1.4 fold increase in thymidine incorporation. This effect was markedly amplified by FSH (3-fold) and estradiol (4.3-fold) and by a combination of both (8.7-fold). The peptide containing the cell-binding domain of fibronectin (RGDSPC) partially inhibited thymidine incorporation stimulated by TGFβ1. Fibronectin did not synergize with FSH, and the interaction between TGFβ1 and FSH was even observed in the presence of this protein. The conclusions reached were as follows: 1) TGFβ1 is an autocrine stimulator of rat granulosa cell DNA synthesis, 2) FSH and estradiol produce a suppression of latent and active TGFβ production but markedly amplify TGFβ action, presumably at a postreceptor level, and 3) the stimulatory effects of TGFβ1 may be only partly mediated by the increased fibronectin secretion. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00063363_v66_n6_p1862_Saragueta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Estradiol Follicle-stimulating hormone Granulosa cells Growth factors estradiol fibronectin follitropin thymidine transforming growth factor beta1 arginyl-glycyl-aspartic acid arginylglycylaspartic acid DNA estradiol fibronectin follitropin oligopeptide peptide fragment Tgfb1 protein, rat transforming growth factor beta transforming growth factor beta1 animal cell article autocrine effect cell proliferation controlled study DNA synthesis female granulosa cell hormone action nonhuman priority journal protein binding protein domain rat animal binding site biosynthesis cell culture cell division chemistry culture medium cytology drug antagonism drug effect drug interaction metabolism physiology Sprague Dawley rat Animalia Animals Binding Sites Cell Division Cells, Cultured Culture Media, Conditioned DNA Drug Interactions Estradiol Female Fibronectins Follicle Stimulating Hormone Granulosa Cells Oligopeptides Peptide Fragments Rats Rats, Sprague-Dawley Transforming Growth Factor beta Transforming Growth Factor beta1
spellingShingle	Estradiol Follicle-stimulating hormone Granulosa cells Growth factors estradiol fibronectin follitropin thymidine transforming growth factor beta1 arginyl-glycyl-aspartic acid arginylglycylaspartic acid DNA estradiol fibronectin follitropin oligopeptide peptide fragment Tgfb1 protein, rat transforming growth factor beta transforming growth factor beta1 animal cell article autocrine effect cell proliferation controlled study DNA synthesis female granulosa cell hormone action nonhuman priority journal protein binding protein domain rat animal binding site biosynthesis cell culture cell division chemistry culture medium cytology drug antagonism drug effect drug interaction metabolism physiology Sprague Dawley rat Animalia Animals Binding Sites Cell Division Cells, Cultured Culture Media, Conditioned DNA Drug Interactions Estradiol Female Fibronectins Follicle Stimulating Hormone Granulosa Cells Oligopeptides Peptide Fragments Rats Rats, Sprague-Dawley Transforming Growth Factor beta Transforming Growth Factor beta1 Saragüeta, P.E. Lanuza, G.M. Barañao, J.L. Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation
topic_facet	Estradiol Follicle-stimulating hormone Granulosa cells Growth factors estradiol fibronectin follitropin thymidine transforming growth factor beta1 arginyl-glycyl-aspartic acid arginylglycylaspartic acid DNA estradiol fibronectin follitropin oligopeptide peptide fragment Tgfb1 protein, rat transforming growth factor beta transforming growth factor beta1 animal cell article autocrine effect cell proliferation controlled study DNA synthesis female granulosa cell hormone action nonhuman priority journal protein binding protein domain rat animal binding site biosynthesis cell culture cell division chemistry culture medium cytology drug antagonism drug effect drug interaction metabolism physiology Sprague Dawley rat Animalia Animals Binding Sites Cell Division Cells, Cultured Culture Media, Conditioned DNA Drug Interactions Estradiol Female Fibronectins Follicle Stimulating Hormone Granulosa Cells Oligopeptides Peptide Fragments Rats Rats, Sprague-Dawley Transforming Growth Factor beta Transforming Growth Factor beta1
description	We evaluated the effects of transforming growth factor β1 (TGFβ1), alone or in combination with FSH and estradiol, on DNA synthesis in primary cultures of immature rat granulosa cells. 3H-Thymidine incorporation was significantly stimulated by TGFβ1 (5.6-fold). This effect was enhanced by FSH (20 ng/ml, 27.7-fold) or estradiol (100 ng/ml, 13.4-fold) or by a combination of both hormones (59.2-fold). Measurement of TGFβ bioactivity showed the presence of significant amounts of TGFβ in conditioned medium from granulosa cell cultures, and most of the activity was present in the latent form. FSH alone or in combination with estradiol produced a marked suppression of the production of latent and active TGFβ. Activated conditioned medium from control cultures of granulosa cell elicited a 1.4 fold increase in thymidine incorporation. This effect was markedly amplified by FSH (3-fold) and estradiol (4.3-fold) and by a combination of both (8.7-fold). The peptide containing the cell-binding domain of fibronectin (RGDSPC) partially inhibited thymidine incorporation stimulated by TGFβ1. Fibronectin did not synergize with FSH, and the interaction between TGFβ1 and FSH was even observed in the presence of this protein. The conclusions reached were as follows: 1) TGFβ1 is an autocrine stimulator of rat granulosa cell DNA synthesis, 2) FSH and estradiol produce a suppression of latent and active TGFβ production but markedly amplify TGFβ action, presumably at a postreceptor level, and 3) the stimulatory effects of TGFβ1 may be only partly mediated by the increased fibronectin secretion.
format	JOUR
author	Saragüeta, P.E. Lanuza, G.M. Barañao, J.L.
author_facet	Saragüeta, P.E. Lanuza, G.M. Barañao, J.L.
author_sort	Saragüeta, P.E.
title	Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation
title_short	Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation
title_full	Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation
title_fullStr	Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation
title_full_unstemmed	Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation
title_sort	autocrine role of transforming growth factor β1 on rat granulosa cell proliferation
url	http://hdl.handle.net/20.500.12110/paper_00063363_v66_n6_p1862_Saragueta
work_keys_str_mv	AT saraguetape autocrineroleoftransforminggrowthfactorb1onratgranulosacellproliferation AT lanuzagm autocrineroleoftransforminggrowthfactorb1onratgranulosacellproliferation AT baranaojl autocrineroleoftransforminggrowthfactorb1onratgranulosacellproliferation
_version_	1807315316558004224

Autocrine role of transforming growth factor β1 on rat granulosa cell proliferation

Ejemplares similares