Distinctive cognate sequence discrimination, bound DNA conformation, and binding modes in the E2 C-terminal domains from prototype human and bovine papillomaviruses
The C-terminal DNA binding domain of the E2 protein is involved in transcriptional regulation and DNA replication in papillomaviruses. At low ionic strength, the domain has a tendency to form aggregates, a process readily reversible by the addition of salt. While fluorescence anisotropy measurements...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00062960_v39_n47_p14692_Ferreiro |
| Aporte de: |
| id |
todo:paper_00062960_v39_n47_p14692_Ferreiro |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00062960_v39_n47_p14692_Ferreiro2023-10-03T14:04:22Z Distinctive cognate sequence discrimination, bound DNA conformation, and binding modes in the E2 C-terminal domains from prototype human and bovine papillomaviruses Ferreiro, D.U. Lima, L.M.T.R. Nadra, A.D. Alonso, L.G. Goldbaum, F.A. De Prat-Gay, G. article carboxy terminal sequence conformational transition dimerization DNA conformation DNA replication ionic strength nonhuman oligomerization priority journal protein domain protein folding sequence analysis transactivation transcription regulation Adenovirus E2 Proteins Animals Base Sequence Bovine papillomavirus 1 Cattle Circular Dichroism Consensus Sequence DNA Humans Nucleic Acid Conformation Osmolar Concentration Papillomaviridae Peptide Fragments Protein Binding Protein Folding Protein Structure, Tertiary Solutions The C-terminal DNA binding domain of the E2 protein is involved in transcriptional regulation and DNA replication in papillomaviruses. At low ionic strength, the domain has a tendency to form aggregates, a process readily reversible by the addition of salt. While fluorescence anisotropy measurements show a 1:1 stoichiometry at pH 5.5, we observed that a second HPV-16 E2 C-terminal dimer can bind per DNA site at pH 7.0. This was confirmed by displacement of bis-ANS binding, tryptophan fluorescence, native electrophoresis, and circular dichroism. The two binding events are nonequivalent, with a high-affinity binding involving one E2C dimer per DNA molecule with a K(D) of 0.18 ± 0.02 nM and a lower affinity binding mode of 2.0 ± 0.2 nM. The bovine (BPV-1) E2 C-terminal domain binds to an HPV-16 E2 site with 350-fold lower affinity than the human cognate domain and binds 7-fold less tightly even to a bovine-derived DNA site. The ability to discriminate between cognate and noncognate sequences is 50-fold higher for the human domain, and the latter is 180-fold better than the bovine at discriminating specific from nonspecific DNA. A substantial conformational change in bound DNA is observed by near-UV circular dichroism. The bovine domain imposes a different DNA conformation than that caused by the human counterpart, which could be explained by a more pronounced bent. Structure-function differences and biochemical properties of the complexes depend on the protein domain rather than on the DNA, in line with crystallographic evidence. Despite the strong sequence homology and overall folding topology, the differences observed may explain the distinctive transcriptional regulation in bovine and human viruses. Fil:Ferreiro, D.U. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nadra, A.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alonso, L.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Prat-Gay, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00062960_v39_n47_p14692_Ferreiro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
article carboxy terminal sequence conformational transition dimerization DNA conformation DNA replication ionic strength nonhuman oligomerization priority journal protein domain protein folding sequence analysis transactivation transcription regulation Adenovirus E2 Proteins Animals Base Sequence Bovine papillomavirus 1 Cattle Circular Dichroism Consensus Sequence DNA Humans Nucleic Acid Conformation Osmolar Concentration Papillomaviridae Peptide Fragments Protein Binding Protein Folding Protein Structure, Tertiary Solutions |
| spellingShingle |
article carboxy terminal sequence conformational transition dimerization DNA conformation DNA replication ionic strength nonhuman oligomerization priority journal protein domain protein folding sequence analysis transactivation transcription regulation Adenovirus E2 Proteins Animals Base Sequence Bovine papillomavirus 1 Cattle Circular Dichroism Consensus Sequence DNA Humans Nucleic Acid Conformation Osmolar Concentration Papillomaviridae Peptide Fragments Protein Binding Protein Folding Protein Structure, Tertiary Solutions Ferreiro, D.U. Lima, L.M.T.R. Nadra, A.D. Alonso, L.G. Goldbaum, F.A. De Prat-Gay, G. Distinctive cognate sequence discrimination, bound DNA conformation, and binding modes in the E2 C-terminal domains from prototype human and bovine papillomaviruses |
| topic_facet |
article carboxy terminal sequence conformational transition dimerization DNA conformation DNA replication ionic strength nonhuman oligomerization priority journal protein domain protein folding sequence analysis transactivation transcription regulation Adenovirus E2 Proteins Animals Base Sequence Bovine papillomavirus 1 Cattle Circular Dichroism Consensus Sequence DNA Humans Nucleic Acid Conformation Osmolar Concentration Papillomaviridae Peptide Fragments Protein Binding Protein Folding Protein Structure, Tertiary Solutions |
| description |
The C-terminal DNA binding domain of the E2 protein is involved in transcriptional regulation and DNA replication in papillomaviruses. At low ionic strength, the domain has a tendency to form aggregates, a process readily reversible by the addition of salt. While fluorescence anisotropy measurements show a 1:1 stoichiometry at pH 5.5, we observed that a second HPV-16 E2 C-terminal dimer can bind per DNA site at pH 7.0. This was confirmed by displacement of bis-ANS binding, tryptophan fluorescence, native electrophoresis, and circular dichroism. The two binding events are nonequivalent, with a high-affinity binding involving one E2C dimer per DNA molecule with a K(D) of 0.18 ± 0.02 nM and a lower affinity binding mode of 2.0 ± 0.2 nM. The bovine (BPV-1) E2 C-terminal domain binds to an HPV-16 E2 site with 350-fold lower affinity than the human cognate domain and binds 7-fold less tightly even to a bovine-derived DNA site. The ability to discriminate between cognate and noncognate sequences is 50-fold higher for the human domain, and the latter is 180-fold better than the bovine at discriminating specific from nonspecific DNA. A substantial conformational change in bound DNA is observed by near-UV circular dichroism. The bovine domain imposes a different DNA conformation than that caused by the human counterpart, which could be explained by a more pronounced bent. Structure-function differences and biochemical properties of the complexes depend on the protein domain rather than on the DNA, in line with crystallographic evidence. Despite the strong sequence homology and overall folding topology, the differences observed may explain the distinctive transcriptional regulation in bovine and human viruses. |
| format |
JOUR |
| author |
Ferreiro, D.U. Lima, L.M.T.R. Nadra, A.D. Alonso, L.G. Goldbaum, F.A. De Prat-Gay, G. |
| author_facet |
Ferreiro, D.U. Lima, L.M.T.R. Nadra, A.D. Alonso, L.G. Goldbaum, F.A. De Prat-Gay, G. |
| author_sort |
Ferreiro, D.U. |
| title |
Distinctive cognate sequence discrimination, bound DNA conformation, and binding modes in the E2 C-terminal domains from prototype human and bovine papillomaviruses |
| title_short |
Distinctive cognate sequence discrimination, bound DNA conformation, and binding modes in the E2 C-terminal domains from prototype human and bovine papillomaviruses |
| title_full |
Distinctive cognate sequence discrimination, bound DNA conformation, and binding modes in the E2 C-terminal domains from prototype human and bovine papillomaviruses |
| title_fullStr |
Distinctive cognate sequence discrimination, bound DNA conformation, and binding modes in the E2 C-terminal domains from prototype human and bovine papillomaviruses |
| title_full_unstemmed |
Distinctive cognate sequence discrimination, bound DNA conformation, and binding modes in the E2 C-terminal domains from prototype human and bovine papillomaviruses |
| title_sort |
distinctive cognate sequence discrimination, bound dna conformation, and binding modes in the e2 c-terminal domains from prototype human and bovine papillomaviruses |
| url |
http://hdl.handle.net/20.500.12110/paper_00062960_v39_n47_p14692_Ferreiro |
| work_keys_str_mv |
AT ferreirodu distinctivecognatesequencediscriminationbounddnaconformationandbindingmodesinthee2cterminaldomainsfromprototypehumanandbovinepapillomaviruses AT limalmtr distinctivecognatesequencediscriminationbounddnaconformationandbindingmodesinthee2cterminaldomainsfromprototypehumanandbovinepapillomaviruses AT nadraad distinctivecognatesequencediscriminationbounddnaconformationandbindingmodesinthee2cterminaldomainsfromprototypehumanandbovinepapillomaviruses AT alonsolg distinctivecognatesequencediscriminationbounddnaconformationandbindingmodesinthee2cterminaldomainsfromprototypehumanandbovinepapillomaviruses AT goldbaumfa distinctivecognatesequencediscriminationbounddnaconformationandbindingmodesinthee2cterminaldomainsfromprototypehumanandbovinepapillomaviruses AT depratgayg distinctivecognatesequencediscriminationbounddnaconformationandbindingmodesinthee2cterminaldomainsfromprototypehumanandbovinepapillomaviruses |
| _version_ |
1782025866097721344 |