A dual effect of Ca2+ on chloroplast fructose-1,6-bisphosphatase
Chloroplast fructose-1,6-bisphosphatase, isolated from spinach leaves, was activated by preincubation with Ca2+ (or Mn2+), fructose-1,6-bisphosphate and dithiothreitol-reduced thioredoxin-f. Upon activation, the enzyme displayed high activity when measured at low concentrations of both fructose-1,6-...
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| Autores principales: | , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0006291x_v97_n1_p325_Hertig |
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| Sumario: | Chloroplast fructose-1,6-bisphosphatase, isolated from spinach leaves, was activated by preincubation with Ca2+ (or Mn2+), fructose-1,6-bisphosphate and dithiothreitol-reduced thioredoxin-f. Upon activation, the enzyme displayed high activity when measured at low concentrations of both fructose-1,6-bisphosphate and Mg2+. On the contrary, the activity of chloroplast fructose-1,6-bisphosphatase was inhibited by Ca2+. These results suggest that Ca2+ (or Mn2+) is potentially important in the regulation of the chloroplast fructose-1,6-bisphosphatase reaction (activation and catalysis). © 1980, Academic Press, Inc.. All rights reserved. |
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