A dual effect of Ca2+ on chloroplast fructose-1,6-bisphosphatase
Chloroplast fructose-1,6-bisphosphatase, isolated from spinach leaves, was activated by preincubation with Ca2+ (or Mn2+), fructose-1,6-bisphosphate and dithiothreitol-reduced thioredoxin-f. Upon activation, the enzyme displayed high activity when measured at low concentrations of both fructose-1,6-...
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todo:paper_0006291x_v97_n1_p325_Hertig2023-10-03T14:04:12Z A dual effect of Ca2+ on chloroplast fructose-1,6-bisphosphatase Hertig, C. Wolosiuk, R.A. D-fructose-1,6-bisphosphate D-fructose-6-phosphate dithiothreitol DTT EGTA ethylene glycol bis-(β-amino-ethyl ether) N,N,N′,N′ tetraacetic acid Fru-1,6-P2 : Fru-6-P Fru-P2ase fructose-1,6-bisphosphatase (EC 3.1.3.11) inorganic phosphate Pi TRIS Tris-(hydroxymethyl) amino-methane calcium dithiothreitol egtazic acid fructose bisphosphatase manganese calcium enzyme fructose bisphosphatase fructose bisphosphate manganese article chloroplast enzyme activation enzymology kinetics metabolism plant animal experiment chloroplast higher plant in vitro study preliminary communication Calcium Chloroplasts Dithiothreitol Egtazic Acid Enzyme Activation Fructose-Bisphosphatase Kinetics Manganese Plants Support, Non-U.S. Gov't Animalia Embryophyta Chloroplast fructose-1,6-bisphosphatase, isolated from spinach leaves, was activated by preincubation with Ca2+ (or Mn2+), fructose-1,6-bisphosphate and dithiothreitol-reduced thioredoxin-f. Upon activation, the enzyme displayed high activity when measured at low concentrations of both fructose-1,6-bisphosphate and Mg2+. On the contrary, the activity of chloroplast fructose-1,6-bisphosphatase was inhibited by Ca2+. These results suggest that Ca2+ (or Mn2+) is potentially important in the regulation of the chloroplast fructose-1,6-bisphosphatase reaction (activation and catalysis). © 1980, Academic Press, Inc.. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291x_v97_n1_p325_Hertig |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
D-fructose-1,6-bisphosphate D-fructose-6-phosphate dithiothreitol DTT EGTA ethylene glycol bis-(β-amino-ethyl ether) N,N,N′,N′ tetraacetic acid Fru-1,6-P2 : Fru-6-P Fru-P2ase fructose-1,6-bisphosphatase (EC 3.1.3.11) inorganic phosphate Pi TRIS Tris-(hydroxymethyl) amino-methane calcium dithiothreitol egtazic acid fructose bisphosphatase manganese calcium enzyme fructose bisphosphatase fructose bisphosphate manganese article chloroplast enzyme activation enzymology kinetics metabolism plant animal experiment chloroplast higher plant in vitro study preliminary communication Calcium Chloroplasts Dithiothreitol Egtazic Acid Enzyme Activation Fructose-Bisphosphatase Kinetics Manganese Plants Support, Non-U.S. Gov't Animalia Embryophyta |
| spellingShingle |
D-fructose-1,6-bisphosphate D-fructose-6-phosphate dithiothreitol DTT EGTA ethylene glycol bis-(β-amino-ethyl ether) N,N,N′,N′ tetraacetic acid Fru-1,6-P2 : Fru-6-P Fru-P2ase fructose-1,6-bisphosphatase (EC 3.1.3.11) inorganic phosphate Pi TRIS Tris-(hydroxymethyl) amino-methane calcium dithiothreitol egtazic acid fructose bisphosphatase manganese calcium enzyme fructose bisphosphatase fructose bisphosphate manganese article chloroplast enzyme activation enzymology kinetics metabolism plant animal experiment chloroplast higher plant in vitro study preliminary communication Calcium Chloroplasts Dithiothreitol Egtazic Acid Enzyme Activation Fructose-Bisphosphatase Kinetics Manganese Plants Support, Non-U.S. Gov't Animalia Embryophyta Hertig, C. Wolosiuk, R.A. A dual effect of Ca2+ on chloroplast fructose-1,6-bisphosphatase |
| topic_facet |
D-fructose-1,6-bisphosphate D-fructose-6-phosphate dithiothreitol DTT EGTA ethylene glycol bis-(β-amino-ethyl ether) N,N,N′,N′ tetraacetic acid Fru-1,6-P2 : Fru-6-P Fru-P2ase fructose-1,6-bisphosphatase (EC 3.1.3.11) inorganic phosphate Pi TRIS Tris-(hydroxymethyl) amino-methane calcium dithiothreitol egtazic acid fructose bisphosphatase manganese calcium enzyme fructose bisphosphatase fructose bisphosphate manganese article chloroplast enzyme activation enzymology kinetics metabolism plant animal experiment chloroplast higher plant in vitro study preliminary communication Calcium Chloroplasts Dithiothreitol Egtazic Acid Enzyme Activation Fructose-Bisphosphatase Kinetics Manganese Plants Support, Non-U.S. Gov't Animalia Embryophyta |
| description |
Chloroplast fructose-1,6-bisphosphatase, isolated from spinach leaves, was activated by preincubation with Ca2+ (or Mn2+), fructose-1,6-bisphosphate and dithiothreitol-reduced thioredoxin-f. Upon activation, the enzyme displayed high activity when measured at low concentrations of both fructose-1,6-bisphosphate and Mg2+. On the contrary, the activity of chloroplast fructose-1,6-bisphosphatase was inhibited by Ca2+. These results suggest that Ca2+ (or Mn2+) is potentially important in the regulation of the chloroplast fructose-1,6-bisphosphatase reaction (activation and catalysis). © 1980, Academic Press, Inc.. All rights reserved. |
| format |
JOUR |
| author |
Hertig, C. Wolosiuk, R.A. |
| author_facet |
Hertig, C. Wolosiuk, R.A. |
| author_sort |
Hertig, C. |
| title |
A dual effect of Ca2+ on chloroplast fructose-1,6-bisphosphatase |
| title_short |
A dual effect of Ca2+ on chloroplast fructose-1,6-bisphosphatase |
| title_full |
A dual effect of Ca2+ on chloroplast fructose-1,6-bisphosphatase |
| title_fullStr |
A dual effect of Ca2+ on chloroplast fructose-1,6-bisphosphatase |
| title_full_unstemmed |
A dual effect of Ca2+ on chloroplast fructose-1,6-bisphosphatase |
| title_sort |
dual effect of ca2+ on chloroplast fructose-1,6-bisphosphatase |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291x_v97_n1_p325_Hertig |
| work_keys_str_mv |
AT hertigc adualeffectofca2onchloroplastfructose16bisphosphatase AT wolosiukra adualeffectofca2onchloroplastfructose16bisphosphatase AT hertigc dualeffectofca2onchloroplastfructose16bisphosphatase AT wolosiukra dualeffectofca2onchloroplastfructose16bisphosphatase |
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1807320768800882688 |