GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process

Yeast cells are able to adapt their metabolism according to the quality of both carbon and nitrogen sources available in the environment. Saccharomyces cerevisiae UGA4 gene encodes a permease capable of transporting γ-aminobutyric acid (GABA) into the cells. Yeast uses this amino acid as a nitrogen...

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Autores principales:	Levi, C.E., Cardillo, S.B., Bertotti, S., Ríos, C., Correa García, S., Moretti, M.B.
Formato:	JOUR
Materias:	Carbon regulation Dal81 GATA factors Kinases Uga3 UGA4 4 aminobutyric acid carbon fungal protein transcription factor uga4 protein unclassified drug article carbon source gene induction nonhuman priority journal protein expression protein localization protein phosphorylation Saccharomyces cerevisiae Carbon DNA-Binding Proteins Fermentation GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Glucose Phosphatidylinositol 3-Kinases Promoter Regions, Genetic Protein-Serine-Threonine Kinases Saccharomyces cerevisiae Proteins Transcription Factors
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0006291X_v421_n3_p572_Levi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0006291X_v421_n3_p572_Levi
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spelling	todo:paper_0006291X_v421_n3_p572_Levi2023-10-03T14:04:00Z GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process Levi, C.E. Cardillo, S.B. Bertotti, S. Ríos, C. Correa García, S. Moretti, M.B. Carbon regulation Dal81 GATA factors Kinases Uga3 UGA4 4 aminobutyric acid carbon fungal protein transcription factor uga4 protein unclassified drug article carbon source gene induction nonhuman priority journal protein expression protein localization protein phosphorylation Saccharomyces cerevisiae Carbon DNA-Binding Proteins Fermentation GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Glucose Phosphatidylinositol 3-Kinases Promoter Regions, Genetic Protein-Serine-Threonine Kinases Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae Yeast cells are able to adapt their metabolism according to the quality of both carbon and nitrogen sources available in the environment. Saccharomyces cerevisiae UGA4 gene encodes a permease capable of transporting γ-aminobutyric acid (GABA) into the cells. Yeast uses this amino acid as a nitrogen source or as a carbon skeleton that enters the tricarboxylic acid cycle.The quality of the carbon source modulates UGA4 expression through two parallel pathways, each one acting on different regulatory elements, the UAS GATA and the UAS GABA. In the presence of a fermentable carbon source, UGA4 expression is induced by GABA while in the presence of a non-fermentable carbon source this expression is GABA-independent.The aim of this work was to study the mechanisms responsible for the differences in the profiles of UGA4 expression in both growth conditions.We found that although the subcellular localization of Gln3 depends on the carbon source and UGA4 expression depends on Tor1 and Snf1, Gln3 localization does not depend on these kinases. We also found that the phosphorylation of Gln3 is mediated by two systems activated by a non-fermentable carbon source, involving the Snf1 kinase and an unidentified TORC1-regulated kinase.We also found that the activity of the main transcription factors responsible for UGA4 induction by GABA varies depending on the quality of the carbon source. In a fermentable carbon source such as glucose, the negative GATA factor Dal80 binds to UGA4 promoter; only after the addition of the inducer, the positive factors Uga3, Dal81 and Gln3 interact with the promoter removing Dal80 and leading to gene induction. In contrast, in the non-fermentable carbon source acetate the negative GATA factor remains bound to UGA4 promoter in the presence or absence of GABA, the positive factors are not detected bound in any of these conditions and in consequence, UGA4 is not induced. © 2012 Elsevier Inc. Fil:Cardillo, S.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bertotti, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v421_n3_p572_Levi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Carbon regulation Dal81 GATA factors Kinases Uga3 UGA4 4 aminobutyric acid carbon fungal protein transcription factor uga4 protein unclassified drug article carbon source gene induction nonhuman priority journal protein expression protein localization protein phosphorylation Saccharomyces cerevisiae Carbon DNA-Binding Proteins Fermentation GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Glucose Phosphatidylinositol 3-Kinases Promoter Regions, Genetic Protein-Serine-Threonine Kinases Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae
spellingShingle	Carbon regulation Dal81 GATA factors Kinases Uga3 UGA4 4 aminobutyric acid carbon fungal protein transcription factor uga4 protein unclassified drug article carbon source gene induction nonhuman priority journal protein expression protein localization protein phosphorylation Saccharomyces cerevisiae Carbon DNA-Binding Proteins Fermentation GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Glucose Phosphatidylinositol 3-Kinases Promoter Regions, Genetic Protein-Serine-Threonine Kinases Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae Levi, C.E. Cardillo, S.B. Bertotti, S. Ríos, C. Correa García, S. Moretti, M.B. GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
topic_facet	Carbon regulation Dal81 GATA factors Kinases Uga3 UGA4 4 aminobutyric acid carbon fungal protein transcription factor uga4 protein unclassified drug article carbon source gene induction nonhuman priority journal protein expression protein localization protein phosphorylation Saccharomyces cerevisiae Carbon DNA-Binding Proteins Fermentation GABA Plasma Membrane Transport Proteins gamma-Aminobutyric Acid Gene Expression Regulation, Developmental Gene Expression Regulation, Fungal Glucose Phosphatidylinositol 3-Kinases Promoter Regions, Genetic Protein-Serine-Threonine Kinases Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Saccharomyces cerevisiae
description	Yeast cells are able to adapt their metabolism according to the quality of both carbon and nitrogen sources available in the environment. Saccharomyces cerevisiae UGA4 gene encodes a permease capable of transporting γ-aminobutyric acid (GABA) into the cells. Yeast uses this amino acid as a nitrogen source or as a carbon skeleton that enters the tricarboxylic acid cycle.The quality of the carbon source modulates UGA4 expression through two parallel pathways, each one acting on different regulatory elements, the UAS GATA and the UAS GABA. In the presence of a fermentable carbon source, UGA4 expression is induced by GABA while in the presence of a non-fermentable carbon source this expression is GABA-independent.The aim of this work was to study the mechanisms responsible for the differences in the profiles of UGA4 expression in both growth conditions.We found that although the subcellular localization of Gln3 depends on the carbon source and UGA4 expression depends on Tor1 and Snf1, Gln3 localization does not depend on these kinases. We also found that the phosphorylation of Gln3 is mediated by two systems activated by a non-fermentable carbon source, involving the Snf1 kinase and an unidentified TORC1-regulated kinase.We also found that the activity of the main transcription factors responsible for UGA4 induction by GABA varies depending on the quality of the carbon source. In a fermentable carbon source such as glucose, the negative GATA factor Dal80 binds to UGA4 promoter; only after the addition of the inducer, the positive factors Uga3, Dal81 and Gln3 interact with the promoter removing Dal80 and leading to gene induction. In contrast, in the non-fermentable carbon source acetate the negative GATA factor remains bound to UGA4 promoter in the presence or absence of GABA, the positive factors are not detected bound in any of these conditions and in consequence, UGA4 is not induced. © 2012 Elsevier Inc.
format	JOUR
author	Levi, C.E. Cardillo, S.B. Bertotti, S. Ríos, C. Correa García, S. Moretti, M.B.
author_facet	Levi, C.E. Cardillo, S.B. Bertotti, S. Ríos, C. Correa García, S. Moretti, M.B.
author_sort	Levi, C.E.
title	GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_short	GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_full	GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_fullStr	GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_full_unstemmed	GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process
title_sort	gaba induction of the saccharomyces cerevisiae uga4 gene depends on the quality of the carbon source: role of the key transcription factors acting in this process
url	http://hdl.handle.net/20.500.12110/paper_0006291X_v421_n3_p572_Levi
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GABA induction of the Saccharomyces cerevisiae UGA4 gene depends on the quality of the carbon source: Role of the key transcription factors acting in this process

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