Common features and differences in the expression of the three genes forming the UGA regulon in Saccharomyces cerevisiae
The three genes that form the UGA regulon in Saccharomyces cerevisiae are responsible for the transport and degradation of γ-aminobutyric acid (GABA) in this organism. Despite the differences in the sequence of their promoters, these genes similarly respond to GABA stimuli. The expression of UGA1, U...
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todo:paper_0006291X_v410_n4_p885_Cardillo2023-10-03T14:03:59Z Common features and differences in the expression of the three genes forming the UGA regulon in Saccharomyces cerevisiae Cardillo, S.B. Correa García, S. Bermúdez Moretti, M. Leu3 SPS amino acid sensor UGA regulon Uga35/Dal81 4 aminobutyric acid amino acid transcription factor transcription factor Leu3 transcription factor UGA1 transcription factor UGA2 transcription factor UGA3 transcription factor UGA4 transcription factor UGA5 unclassified drug article gene control gene expression gene induction gene sequence nitrogen catabolite repression nonhuman priority journal protein processing regulon Saccharomyces cerevisiae 4-Aminobutyrate Transaminase Down-Regulation GABA Plasma Membrane Transport Proteins Gene Expression Regulation, Fungal Leucine Regulon Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Succinate-Semialdehyde Dehydrogenase (NADP+) Trans-Activators Transcription Factors Saccharomyces cerevisiae The three genes that form the UGA regulon in Saccharomyces cerevisiae are responsible for the transport and degradation of γ-aminobutyric acid (GABA) in this organism. Despite the differences in the sequence of their promoters, these genes similarly respond to GABA stimuli. The expression of UGA1, UGA2 and UGA4 depends on GABA induction and nitrogen catabolite repression (NCR). The induction of these genes requires the action of at least two positive proteins, the specific Uga3 and the pleiotropic Uga35/Dal81 transcription factors. Here we show that all the members of the UGA regulon, as was already demonstrated for UGA4, are negatively regulated by extracellular amino acids through the SPS amino acid sensor. We also show that this negative effect is caused by a low availability of Uga35/Dal81 transcription factor and that Leu3 transcription factor negatively regulates UGA4 and UGA1 expression but it does not affect UGA2 expression. © 2011 Elsevier Inc. Fil:Cardillo, S.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n4_p885_Cardillo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Leu3 SPS amino acid sensor UGA regulon Uga35/Dal81 4 aminobutyric acid amino acid transcription factor transcription factor Leu3 transcription factor UGA1 transcription factor UGA2 transcription factor UGA3 transcription factor UGA4 transcription factor UGA5 unclassified drug article gene control gene expression gene induction gene sequence nitrogen catabolite repression nonhuman priority journal protein processing regulon Saccharomyces cerevisiae 4-Aminobutyrate Transaminase Down-Regulation GABA Plasma Membrane Transport Proteins Gene Expression Regulation, Fungal Leucine Regulon Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Succinate-Semialdehyde Dehydrogenase (NADP+) Trans-Activators Transcription Factors Saccharomyces cerevisiae |
| spellingShingle |
Leu3 SPS amino acid sensor UGA regulon Uga35/Dal81 4 aminobutyric acid amino acid transcription factor transcription factor Leu3 transcription factor UGA1 transcription factor UGA2 transcription factor UGA3 transcription factor UGA4 transcription factor UGA5 unclassified drug article gene control gene expression gene induction gene sequence nitrogen catabolite repression nonhuman priority journal protein processing regulon Saccharomyces cerevisiae 4-Aminobutyrate Transaminase Down-Regulation GABA Plasma Membrane Transport Proteins Gene Expression Regulation, Fungal Leucine Regulon Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Succinate-Semialdehyde Dehydrogenase (NADP+) Trans-Activators Transcription Factors Saccharomyces cerevisiae Cardillo, S.B. Correa García, S. Bermúdez Moretti, M. Common features and differences in the expression of the three genes forming the UGA regulon in Saccharomyces cerevisiae |
| topic_facet |
Leu3 SPS amino acid sensor UGA regulon Uga35/Dal81 4 aminobutyric acid amino acid transcription factor transcription factor Leu3 transcription factor UGA1 transcription factor UGA2 transcription factor UGA3 transcription factor UGA4 transcription factor UGA5 unclassified drug article gene control gene expression gene induction gene sequence nitrogen catabolite repression nonhuman priority journal protein processing regulon Saccharomyces cerevisiae 4-Aminobutyrate Transaminase Down-Regulation GABA Plasma Membrane Transport Proteins Gene Expression Regulation, Fungal Leucine Regulon Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Succinate-Semialdehyde Dehydrogenase (NADP+) Trans-Activators Transcription Factors Saccharomyces cerevisiae |
| description |
The three genes that form the UGA regulon in Saccharomyces cerevisiae are responsible for the transport and degradation of γ-aminobutyric acid (GABA) in this organism. Despite the differences in the sequence of their promoters, these genes similarly respond to GABA stimuli. The expression of UGA1, UGA2 and UGA4 depends on GABA induction and nitrogen catabolite repression (NCR). The induction of these genes requires the action of at least two positive proteins, the specific Uga3 and the pleiotropic Uga35/Dal81 transcription factors. Here we show that all the members of the UGA regulon, as was already demonstrated for UGA4, are negatively regulated by extracellular amino acids through the SPS amino acid sensor. We also show that this negative effect is caused by a low availability of Uga35/Dal81 transcription factor and that Leu3 transcription factor negatively regulates UGA4 and UGA1 expression but it does not affect UGA2 expression. © 2011 Elsevier Inc. |
| format |
JOUR |
| author |
Cardillo, S.B. Correa García, S. Bermúdez Moretti, M. |
| author_facet |
Cardillo, S.B. Correa García, S. Bermúdez Moretti, M. |
| author_sort |
Cardillo, S.B. |
| title |
Common features and differences in the expression of the three genes forming the UGA regulon in Saccharomyces cerevisiae |
| title_short |
Common features and differences in the expression of the three genes forming the UGA regulon in Saccharomyces cerevisiae |
| title_full |
Common features and differences in the expression of the three genes forming the UGA regulon in Saccharomyces cerevisiae |
| title_fullStr |
Common features and differences in the expression of the three genes forming the UGA regulon in Saccharomyces cerevisiae |
| title_full_unstemmed |
Common features and differences in the expression of the three genes forming the UGA regulon in Saccharomyces cerevisiae |
| title_sort |
common features and differences in the expression of the three genes forming the uga regulon in saccharomyces cerevisiae |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n4_p885_Cardillo |
| work_keys_str_mv |
AT cardillosb commonfeaturesanddifferencesintheexpressionofthethreegenesformingtheugareguloninsaccharomycescerevisiae AT correagarcias commonfeaturesanddifferencesintheexpressionofthethreegenesformingtheugareguloninsaccharomycescerevisiae AT bermudezmorettim commonfeaturesanddifferencesintheexpressionofthethreegenesformingtheugareguloninsaccharomycescerevisiae |
| _version_ |
1782026291318358016 |