Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium

Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing i...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Solari, C., Losino, N., Luzzani, C., Waisman, A., Bluguermann, C., Questa, M., Sevlever, G., Miriuka, S., Barañao, L., Guberman, A.
Formato: JOUR
Materias:
BGC
BGC-CM
CM
Conditioned medium
Culture conditions
ESC
GC
Induced pluripotent stem cell
IPSC
LIF
MEF
MESC
Pluripotency
Self-renewal
SM
animal cell
article
cell differentiation
cell type
controlled study
fibroblast
gene expression
granulosa cell
immunofluorescence
in vitro study
in vivo study
Lentivirinae
marker gene
mouse
nonhuman
pluripotent stem cell
priority journal
Animals
Cattle
Cell Culture Techniques
Cell Line
Culture Media, Conditioned
Female
Granulosa Cells
Induced Pluripotent Stem Cells
Mice
Octamer Transcription Factor-3
Regeneration
Bovinae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n2_p252_Solari
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0006291X_v410_n2_p252_Solari
record_format dspace
spelling todo:paper_0006291X_v410_n2_p252_Solari2023-10-03T14:03:58Z Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium Solari, C. Losino, N. Luzzani, C. Waisman, A. Bluguermann, C. Questa, M. Sevlever, G. Miriuka, S. Barañao, L. Guberman, A. BGC BGC-CM CM Conditioned medium Culture conditions ESC GC Induced pluripotent stem cell IPSC LIF MEF MESC Pluripotency Self-renewal SM animal cell article cell differentiation cell type controlled study fibroblast gene expression granulosa cell immunofluorescence in vitro study in vivo study Lentivirinae marker gene mouse nonhuman pluripotent stem cell priority journal Animals Cattle Cell Culture Techniques Cell Line Culture Media, Conditioned Female Granulosa Cells Induced Pluripotent Stem Cells Mice Octamer Transcription Factor-3 Regeneration Bovinae Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing in vitro models of disease, drug discovery, toxicology and potentially treating degenerative disease circumventing immune rejection. In spite of the great advance since iPSCs' establishment, their obtention and propagation is an increasing area of great interest.In a recent work, we have shown that the conditioned medium from a bovine granulosa cell line (BGC-CM) is able to preserve the basic properties of mESCs. Therefore, based on our previous results and the reported resemblance between iPSCs and ESCs, we hypothesized that BGC-CM could provide a favorable context to culturing iPSCs. In this work, we have reprogrammed mouse embryonic fibroblasts obtaining iPSC lines, and showed that they can be propagated in BGC-CM while maintaining self-renewal and pluripotency, evidenced by expression of specific gene markers and capability of in vitro and in vivo differentiation to cell types from the three germ layers. We believe that these findings may provide a novel context to propagate iPSCs to study the molecular mechanisms involved in self-renewal and pluripotency. © 2011 Elsevier Inc. Fil:Losino, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Waisman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Questa, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n2_p252_Solari
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic BGC
BGC-CM
CM
Conditioned medium
Culture conditions
ESC
GC
Induced pluripotent stem cell
IPSC
LIF
MEF
MESC
Pluripotency
Self-renewal
SM
animal cell
article
cell differentiation
cell type
controlled study
fibroblast
gene expression
granulosa cell
immunofluorescence
in vitro study
in vivo study
Lentivirinae
marker gene
mouse
nonhuman
pluripotent stem cell
priority journal
Animals
Cattle
Cell Culture Techniques
Cell Line
Culture Media, Conditioned
Female
Granulosa Cells
Induced Pluripotent Stem Cells
Mice
Octamer Transcription Factor-3
Regeneration
Bovinae
spellingShingle BGC
BGC-CM
CM
Conditioned medium
Culture conditions
ESC
GC
Induced pluripotent stem cell
IPSC
LIF
MEF
MESC
Pluripotency
Self-renewal
SM
animal cell
article
cell differentiation
cell type
controlled study
fibroblast
gene expression
granulosa cell
immunofluorescence
in vitro study
in vivo study
Lentivirinae
marker gene
mouse
nonhuman
pluripotent stem cell
priority journal
Animals
Cattle
Cell Culture Techniques
Cell Line
Culture Media, Conditioned
Female
Granulosa Cells
Induced Pluripotent Stem Cells
Mice
Octamer Transcription Factor-3
Regeneration
Bovinae
Solari, C.
Losino, N.
Luzzani, C.
Waisman, A.
Bluguermann, C.
Questa, M.
Sevlever, G.
Miriuka, S.
Barañao, L.
Guberman, A.
Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium
topic_facet BGC
BGC-CM
CM
Conditioned medium
Culture conditions
ESC
GC
Induced pluripotent stem cell
IPSC
LIF
MEF
MESC
Pluripotency
Self-renewal
SM
animal cell
article
cell differentiation
cell type
controlled study
fibroblast
gene expression
granulosa cell
immunofluorescence
in vitro study
in vivo study
Lentivirinae
marker gene
mouse
nonhuman
pluripotent stem cell
priority journal
Animals
Cattle
Cell Culture Techniques
Cell Line
Culture Media, Conditioned
Female
Granulosa Cells
Induced Pluripotent Stem Cells
Mice
Octamer Transcription Factor-3
Regeneration
Bovinae
description Induced pluripotent stem cells (iPSCs) are a promising type of stem cells, comparable to embryonic stem cells (ESCs) in terms of self-renew and pluripotency, generated by reprogramming somatic cells. These cells are an attractive approach to supply patient-specific pluripotent cells, for producing in vitro models of disease, drug discovery, toxicology and potentially treating degenerative disease circumventing immune rejection. In spite of the great advance since iPSCs' establishment, their obtention and propagation is an increasing area of great interest.In a recent work, we have shown that the conditioned medium from a bovine granulosa cell line (BGC-CM) is able to preserve the basic properties of mESCs. Therefore, based on our previous results and the reported resemblance between iPSCs and ESCs, we hypothesized that BGC-CM could provide a favorable context to culturing iPSCs. In this work, we have reprogrammed mouse embryonic fibroblasts obtaining iPSC lines, and showed that they can be propagated in BGC-CM while maintaining self-renewal and pluripotency, evidenced by expression of specific gene markers and capability of in vitro and in vivo differentiation to cell types from the three germ layers. We believe that these findings may provide a novel context to propagate iPSCs to study the molecular mechanisms involved in self-renewal and pluripotency. © 2011 Elsevier Inc.
format JOUR
author Solari, C.
Losino, N.
Luzzani, C.
Waisman, A.
Bluguermann, C.
Questa, M.
Sevlever, G.
Miriuka, S.
Barañao, L.
Guberman, A.
author_facet Solari, C.
Losino, N.
Luzzani, C.
Waisman, A.
Bluguermann, C.
Questa, M.
Sevlever, G.
Miriuka, S.
Barañao, L.
Guberman, A.
author_sort Solari, C.
title Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium
title_short Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium
title_full Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium
title_fullStr Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium
title_full_unstemmed Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium
title_sort induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium
url http://hdl.handle.net/20.500.12110/paper_0006291X_v410_n2_p252_Solari
work_keys_str_mv AT solaric inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
AT losinon inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
AT luzzanic inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
AT waismana inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
AT bluguermannc inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
AT questam inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
AT sevleverg inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
AT miriukas inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
AT baranaol inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
AT gubermana inducedpluripotentstemcellsselfrenewalandpluripotencyismaintainedbyabovinegranulosacelllineconditionedmedium
_version_ 1807319907266723840

Ejemplares similares