Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi
SF3b155 and p14 are essential components of spliceosome core that recognize the branch point adenosine, a critical step in splicing in eukaryotes. Trypanosomes are unusual since every transcribed gene is processed by trans-splicing instead of cis-splicing. Thus, the trans-spliceosome emerges as an i...
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todo:paper_0006291X_v364_n1_p26_Avila2023-10-03T14:03:55Z Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi Avila, M.L. Bercovich, N. Westergaard, G. Levin, M.J. Vázquez, M.P. Drug targets Protein interaction Trans-splicing alanine messenger RNA protein derivative protein p14 SF3b155 protein unclassified drug article DNA splicing gene mapping nonhuman priority journal protein binding protein processing spliceosome Trypanosoma Trypanosoma cruzi Amino Acid Sequence Animals Humans Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular Phosphoproteins Protein Binding Protein Structure, Tertiary Protozoan Proteins Ribonucleoprotein, U2 Small Nuclear RNA-Binding Proteins Spliceosomes Trypanosoma cruzi Eukaryota Mammalia Trypanosoma cruzi SF3b155 and p14 are essential components of spliceosome core that recognize the branch point adenosine, a critical step in splicing in eukaryotes. Trypanosomes are unusual since every transcribed gene is processed by trans-splicing instead of cis-splicing. Thus, the trans-spliceosome emerges as an interesting anti-parasitic drug target since this process is not present in mammalian hosts. Here, we present the orthologues of these proteins in Trypanosoma cruzi that interact strongly with each other. To define similarities and differences with the human pair, we performed a detailed alanine scan analysis that allowed us to identify the regions and the critical amino acids of T. cruzi SF3b155 involved in interaction with p14. We demonstrate that the T. cruzi SF3b155 interface is larger and contains more complex elements than its human counterpart. Additionally, our results provide the first insights into the core of the putative mRNA processing complex of trypanosomes. © 2007 Elsevier Inc. All rights reserved. Fil:Bercovich, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Westergaard, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vázquez, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v364_n1_p26_Avila |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Drug targets Protein interaction Trans-splicing alanine messenger RNA protein derivative protein p14 SF3b155 protein unclassified drug article DNA splicing gene mapping nonhuman priority journal protein binding protein processing spliceosome Trypanosoma Trypanosoma cruzi Amino Acid Sequence Animals Humans Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular Phosphoproteins Protein Binding Protein Structure, Tertiary Protozoan Proteins Ribonucleoprotein, U2 Small Nuclear RNA-Binding Proteins Spliceosomes Trypanosoma cruzi Eukaryota Mammalia Trypanosoma cruzi |
| spellingShingle |
Drug targets Protein interaction Trans-splicing alanine messenger RNA protein derivative protein p14 SF3b155 protein unclassified drug article DNA splicing gene mapping nonhuman priority journal protein binding protein processing spliceosome Trypanosoma Trypanosoma cruzi Amino Acid Sequence Animals Humans Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular Phosphoproteins Protein Binding Protein Structure, Tertiary Protozoan Proteins Ribonucleoprotein, U2 Small Nuclear RNA-Binding Proteins Spliceosomes Trypanosoma cruzi Eukaryota Mammalia Trypanosoma cruzi Avila, M.L. Bercovich, N. Westergaard, G. Levin, M.J. Vázquez, M.P. Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi |
| topic_facet |
Drug targets Protein interaction Trans-splicing alanine messenger RNA protein derivative protein p14 SF3b155 protein unclassified drug article DNA splicing gene mapping nonhuman priority journal protein binding protein processing spliceosome Trypanosoma Trypanosoma cruzi Amino Acid Sequence Animals Humans Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular Phosphoproteins Protein Binding Protein Structure, Tertiary Protozoan Proteins Ribonucleoprotein, U2 Small Nuclear RNA-Binding Proteins Spliceosomes Trypanosoma cruzi Eukaryota Mammalia Trypanosoma cruzi |
| description |
SF3b155 and p14 are essential components of spliceosome core that recognize the branch point adenosine, a critical step in splicing in eukaryotes. Trypanosomes are unusual since every transcribed gene is processed by trans-splicing instead of cis-splicing. Thus, the trans-spliceosome emerges as an interesting anti-parasitic drug target since this process is not present in mammalian hosts. Here, we present the orthologues of these proteins in Trypanosoma cruzi that interact strongly with each other. To define similarities and differences with the human pair, we performed a detailed alanine scan analysis that allowed us to identify the regions and the critical amino acids of T. cruzi SF3b155 involved in interaction with p14. We demonstrate that the T. cruzi SF3b155 interface is larger and contains more complex elements than its human counterpart. Additionally, our results provide the first insights into the core of the putative mRNA processing complex of trypanosomes. © 2007 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Avila, M.L. Bercovich, N. Westergaard, G. Levin, M.J. Vázquez, M.P. |
| author_facet |
Avila, M.L. Bercovich, N. Westergaard, G. Levin, M.J. Vázquez, M.P. |
| author_sort |
Avila, M.L. |
| title |
Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi |
| title_short |
Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi |
| title_full |
Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi |
| title_fullStr |
Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi |
| title_full_unstemmed |
Mapping of the protein-binding interface between splicing factors SF3b155 and p14 of Trypanosoma cruzi |
| title_sort |
mapping of the protein-binding interface between splicing factors sf3b155 and p14 of trypanosoma cruzi |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v364_n1_p26_Avila |
| work_keys_str_mv |
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| _version_ |
1807324638330486784 |