PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits
Protein kinase A from the fungus Mucor circinelloides shows high affinity interaction between regulatory (R) and catalytic (C) subunits. Its R subunit shows a differential presence of several acidic residues in linker I region, in the amino terminus. Mutants R1, lacking the N-terminal region, and R2...
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todo:paper_0006291X_v362_n3_p721_Ocampo2023-10-03T14:03:54Z PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits Ocampo, J. Moreno, S. Rossi, S. cAMP Interaction R-C Linker I Mucor circinelloides PKA cyclic AMP cyclic AMP dependent protein kinase holoenzyme amino acid sequence amino terminal sequence article catalyst enzyme activity enzyme binding enzyme purification enzyme subunit molecular model Mucor mucor circinelloides nonhuman priority journal protein expression protein interaction Amino Acid Sequence Animals Catalytic Domain Cattle Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dose-Response Relationship, Drug Gene Expression Profiling Kinetics Molecular Sequence Data Mucor Mutagenesis Mutation Phosphorylation Protein Binding Protein Structure, Tertiary Sequence Homology, Amino Acid Bovinae Fungi Mucor circinelloides Protein kinase A from the fungus Mucor circinelloides shows high affinity interaction between regulatory (R) and catalytic (C) subunits. Its R subunit shows a differential presence of several acidic residues in linker I region, in the amino terminus. Mutants R1, lacking the N-terminal region, and R2, lacking the acidic cluster, were used to analyze its effect on the interaction with the C subunit, assessed through inhibition of catalytic activity and cAMP activation of reconstituted holoenzyme. A similar decrease in the interaction was obtained when using R1 and R2 with the homologous C subunit; however when using heterologous bovine C, only R1 had a decreased interaction. The results show the general importance of linker I region in the R-C interaction in protein kinases A and point to the importance of the acidic cluster present in the N-terminus of M. circinelloides R subunit in the high affinity interaction between R and C in this holoenzyme. © 2007 Elsevier Inc. All rights reserved. Fil:Ocampo, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rossi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v362_n3_p721_Ocampo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cAMP Interaction R-C Linker I Mucor circinelloides PKA cyclic AMP cyclic AMP dependent protein kinase holoenzyme amino acid sequence amino terminal sequence article catalyst enzyme activity enzyme binding enzyme purification enzyme subunit molecular model Mucor mucor circinelloides nonhuman priority journal protein expression protein interaction Amino Acid Sequence Animals Catalytic Domain Cattle Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dose-Response Relationship, Drug Gene Expression Profiling Kinetics Molecular Sequence Data Mucor Mutagenesis Mutation Phosphorylation Protein Binding Protein Structure, Tertiary Sequence Homology, Amino Acid Bovinae Fungi Mucor circinelloides |
| spellingShingle |
cAMP Interaction R-C Linker I Mucor circinelloides PKA cyclic AMP cyclic AMP dependent protein kinase holoenzyme amino acid sequence amino terminal sequence article catalyst enzyme activity enzyme binding enzyme purification enzyme subunit molecular model Mucor mucor circinelloides nonhuman priority journal protein expression protein interaction Amino Acid Sequence Animals Catalytic Domain Cattle Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dose-Response Relationship, Drug Gene Expression Profiling Kinetics Molecular Sequence Data Mucor Mutagenesis Mutation Phosphorylation Protein Binding Protein Structure, Tertiary Sequence Homology, Amino Acid Bovinae Fungi Mucor circinelloides Ocampo, J. Moreno, S. Rossi, S. PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits |
| topic_facet |
cAMP Interaction R-C Linker I Mucor circinelloides PKA cyclic AMP cyclic AMP dependent protein kinase holoenzyme amino acid sequence amino terminal sequence article catalyst enzyme activity enzyme binding enzyme purification enzyme subunit molecular model Mucor mucor circinelloides nonhuman priority journal protein expression protein interaction Amino Acid Sequence Animals Catalytic Domain Cattle Cyclic AMP Cyclic AMP-Dependent Protein Kinases Dose-Response Relationship, Drug Gene Expression Profiling Kinetics Molecular Sequence Data Mucor Mutagenesis Mutation Phosphorylation Protein Binding Protein Structure, Tertiary Sequence Homology, Amino Acid Bovinae Fungi Mucor circinelloides |
| description |
Protein kinase A from the fungus Mucor circinelloides shows high affinity interaction between regulatory (R) and catalytic (C) subunits. Its R subunit shows a differential presence of several acidic residues in linker I region, in the amino terminus. Mutants R1, lacking the N-terminal region, and R2, lacking the acidic cluster, were used to analyze its effect on the interaction with the C subunit, assessed through inhibition of catalytic activity and cAMP activation of reconstituted holoenzyme. A similar decrease in the interaction was obtained when using R1 and R2 with the homologous C subunit; however when using heterologous bovine C, only R1 had a decreased interaction. The results show the general importance of linker I region in the R-C interaction in protein kinases A and point to the importance of the acidic cluster present in the N-terminus of M. circinelloides R subunit in the high affinity interaction between R and C in this holoenzyme. © 2007 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Ocampo, J. Moreno, S. Rossi, S. |
| author_facet |
Ocampo, J. Moreno, S. Rossi, S. |
| author_sort |
Ocampo, J. |
| title |
PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits |
| title_short |
PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits |
| title_full |
PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits |
| title_fullStr |
PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits |
| title_full_unstemmed |
PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits |
| title_sort |
pka from mucor circinelloides: model to study the role of linker i in the interaction between r and c subunits |
| url |
http://hdl.handle.net/20.500.12110/paper_0006291X_v362_n3_p721_Ocampo |
| work_keys_str_mv |
AT ocampoj pkafrommucorcircinelloidesmodeltostudytheroleoflinkeriintheinteractionbetweenrandcsubunits AT morenos pkafrommucorcircinelloidesmodeltostudytheroleoflinkeriintheinteractionbetweenrandcsubunits AT rossis pkafrommucorcircinelloidesmodeltostudytheroleoflinkeriintheinteractionbetweenrandcsubunits |
| _version_ |
1807319356821995520 |