PKA from Mucor circinelloides: Model to study the role of linker I in the interaction between R and C subunits

Protein kinase A from the fungus Mucor circinelloides shows high affinity interaction between regulatory (R) and catalytic (C) subunits. Its R subunit shows a differential presence of several acidic residues in linker I region, in the amino terminus. Mutants R1, lacking the N-terminal region, and R2...

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Detalles Bibliográficos
Autores principales: Ocampo, J., Moreno, S., Rossi, S.
Formato: JOUR
Materias:
cAMP
Interaction R-C
Linker I
Mucor circinelloides
PKA
cyclic AMP
cyclic AMP dependent protein kinase
holoenzyme
amino acid sequence
amino terminal sequence
article
catalyst
enzyme activity
enzyme binding
enzyme purification
enzyme subunit
molecular model
Mucor
mucor circinelloides
nonhuman
priority journal
protein expression
protein interaction
Amino Acid Sequence
Animals
Catalytic Domain
Cattle
Cyclic AMP
Cyclic AMP-Dependent Protein Kinases
Dose-Response Relationship, Drug
Gene Expression Profiling
Kinetics
Molecular Sequence Data
Mutagenesis
Mutation
Phosphorylation
Protein Binding
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Bovinae
Fungi
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v362_n3_p721_Ocampo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Protein kinase A from the fungus Mucor circinelloides shows high affinity interaction between regulatory (R) and catalytic (C) subunits. Its R subunit shows a differential presence of several acidic residues in linker I region, in the amino terminus. Mutants R1, lacking the N-terminal region, and R2, lacking the acidic cluster, were used to analyze its effect on the interaction with the C subunit, assessed through inhibition of catalytic activity and cAMP activation of reconstituted holoenzyme. A similar decrease in the interaction was obtained when using R1 and R2 with the homologous C subunit; however when using heterologous bovine C, only R1 had a decreased interaction. The results show the general importance of linker I region in the R-C interaction in protein kinases A and point to the importance of the acidic cluster present in the N-terminus of M. circinelloides R subunit in the high affinity interaction between R and C in this holoenzyme. © 2007 Elsevier Inc. All rights reserved.

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