Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae

In Saccharomyces cerevisiae, as in all eukaryotic organisms, δ-aminolevulinic acid (ALA) is a precursor of porphyrin biosynthesis, a very finely regulated pathway. ALA enters yeast cells through the γ-aminobutyric acid (GABA) permease Uga4. The incorporation of a metabolite into the cells may be a l...

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Autores principales:	Moretti, M.B., Garcia, S.C., Batlle, A.
Formato:	JOUR
Materias:	Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Eukaryota
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0006291X_v272_n3_p946_Moretti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_0006291X_v272_n3_p946_Moretti2023-10-03T14:03:47Z Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae Moretti, M.B. Garcia, S.C. Batlle, A. Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Saccharomyces cerevisiae Eukaryota Saccharomyces cerevisiae Saccharomyces cerevisiae In Saccharomyces cerevisiae, as in all eukaryotic organisms, δ-aminolevulinic acid (ALA) is a precursor of porphyrin biosynthesis, a very finely regulated pathway. ALA enters yeast cells through the γ-aminobutyric acid (GABA) permease Uga4. The incorporation of a metabolite into the cells may be a limiting step for its intracellular metabolization. To determine the relationship between ALA transport and ALA metabolization, ALA incorporation was measured in yeast mutant strains deficient in the δ-aminolevulinic acid-synthase, uroporphyrinogen III decarboxylase, and ferrochelatase, three enzymes involved in porphyrin biosynthesis. Results presented here showed that neither intracellular ALA nor uroporphyrin or protoporphyrin regulates ALA incorporation, indicating that ALA uptake and its subsequent metabolization are not related to each other. Thus a key metabolite as it is, ALA does not have a transport system regulated according to its role. (C) 2000 Academic Press. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v272_n3_p946_Moretti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Saccharomyces cerevisiae Eukaryota Saccharomyces cerevisiae Saccharomyces cerevisiae
spellingShingle	Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Saccharomyces cerevisiae Eukaryota Saccharomyces cerevisiae Saccharomyces cerevisiae Moretti, M.B. Garcia, S.C. Batlle, A. Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
topic_facet	Membrane transport Porphyrin biosynthesis Saccharomyces cerevisiae Transport regulation Uga4 permease γ-aminobutyric acid δ-aminolevulinic acid 4 aminobutyric acid 5 aminolevulinate synthase aminolevulinic acid ferrochelatase permease porphyrin uroporphyrinogen decarboxylase article cell membrane transport cell mutant controlled study enzyme deficiency metabolism nonhuman priority journal regulatory mechanism Saccharomyces cerevisiae Eukaryota Saccharomyces cerevisiae Saccharomyces cerevisiae
description	In Saccharomyces cerevisiae, as in all eukaryotic organisms, δ-aminolevulinic acid (ALA) is a precursor of porphyrin biosynthesis, a very finely regulated pathway. ALA enters yeast cells through the γ-aminobutyric acid (GABA) permease Uga4. The incorporation of a metabolite into the cells may be a limiting step for its intracellular metabolization. To determine the relationship between ALA transport and ALA metabolization, ALA incorporation was measured in yeast mutant strains deficient in the δ-aminolevulinic acid-synthase, uroporphyrinogen III decarboxylase, and ferrochelatase, three enzymes involved in porphyrin biosynthesis. Results presented here showed that neither intracellular ALA nor uroporphyrin or protoporphyrin regulates ALA incorporation, indicating that ALA uptake and its subsequent metabolization are not related to each other. Thus a key metabolite as it is, ALA does not have a transport system regulated according to its role. (C) 2000 Academic Press.
format	JOUR
author	Moretti, M.B. Garcia, S.C. Batlle, A.
author_facet	Moretti, M.B. Garcia, S.C. Batlle, A.
author_sort	Moretti, M.B.
title	Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_short	Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_full	Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_fullStr	Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_full_unstemmed	Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae
title_sort	porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in saccharomyces cerevisiae
url	http://hdl.handle.net/20.500.12110/paper_0006291X_v272_n3_p946_Moretti
work_keys_str_mv	AT morettimb porphyrinbiosynthesisintermediatesarenotregulatingdaminolevulinicacidtransportinsaccharomycescerevisiae AT garciasc porphyrinbiosynthesisintermediatesarenotregulatingdaminolevulinicacidtransportinsaccharomycescerevisiae AT batllea porphyrinbiosynthesisintermediatesarenotregulatingdaminolevulinicacidtransportinsaccharomycescerevisiae
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Porphyrin biosynthesis intermediates are not regulating δ-aminolevulinic acid transport in Saccharomyces cerevisiae

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