In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii

EDTA-treated Rhizobium trifolii cells (strain NA30) incorporate radioactivity from (14C) labeled UDP-Clc, UDP-ClcA, Acetyl-Coa and/or phosphoenol pyruvate into chloroform:methanol:water (1:2:0.3) extracts. The incorporation products have properties of prenyl-phospho-sugars; mild alkaline hydrolysis...

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Autores principales: Bossio, J.C., Inon de Iannino, N., Dankert, M.A.
Formato: JOUR
Materias:
acetic acid
lipid
oligosaccharide
pyruvic acid
nonhuman
priority journal
rhizobium trifolii
Acetylation
Carbohydrate Sequence
Chromatography, Gel
Electrophoresis, Paper
Lipids
Oligosaccharides
Pyruvates
Pyruvic Acid
Rhizobium
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0006291X_v134_n1_p205_Bossio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0006291X_v134_n1_p205_Bossio
record_format dspace
spelling todo:paper_0006291X_v134_n1_p205_Bossio2023-10-03T14:03:38Z In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii Bossio, J.C. Inon de Iannino, N. Dankert, M.A. acetic acid lipid oligosaccharide pyruvic acid nonhuman priority journal rhizobium trifolii Acetylation Carbohydrate Sequence Chromatography, Gel Electrophoresis, Paper Lipids Oligosaccharides Pyruvates Pyruvic Acid Rhizobium Support, Non-U.S. Gov't EDTA-treated Rhizobium trifolii cells (strain NA30) incorporate radioactivity from (14C) labeled UDP-Clc, UDP-ClcA, Acetyl-Coa and/or phosphoenol pyruvate into chloroform:methanol:water (1:2:0.3) extracts. The incorporation products have properties of prenyl-phospho-sugars; mild alkaline hydrolysis of these extracts produce cyclic phosphate esters suggesting the presence of a diphosphate bridge, and mild acid or catalytic reduction-alkaline phosphatase treatments release four main componenets a, b, c and d, as judged by paper electrophoresis and chromatography and gel filtration studies. The four components can be obtained (14C)acetyl-labeled, but only compound c and to a lesser degree compound b can be (14C)pyruvate-labeled. For the exopolysaccharide produced by this strain the following repeating unit has been proposed (Robertsen et al. (1981), Plant Physiol. 67, 389-400): {A figure is presented}. The results obtained suggest that the octasaccharide repeating unit (compound a) with one (compound b) or two (compound c) ketal pyruvate residues are assembled on a lipid acceptor. All these compounds are assumed to be intermediates in the biosynthesis of R. trifolii exopolysaccharide. © 1986. Fil:Bossio, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Dankert, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v134_n1_p205_Bossio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic acetic acid
lipid
oligosaccharide
pyruvic acid
nonhuman
priority journal
rhizobium trifolii
Acetylation
Carbohydrate Sequence
Chromatography, Gel
Electrophoresis, Paper
Lipids
Oligosaccharides
Pyruvates
Pyruvic Acid
Rhizobium
Support, Non-U.S. Gov't
spellingShingle acetic acid
lipid
oligosaccharide
pyruvic acid
nonhuman
priority journal
rhizobium trifolii
Acetylation
Carbohydrate Sequence
Chromatography, Gel
Electrophoresis, Paper
Lipids
Oligosaccharides
Pyruvates
Pyruvic Acid
Rhizobium
Support, Non-U.S. Gov't
Bossio, J.C.
Inon de Iannino, N.
Dankert, M.A.
In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
topic_facet acetic acid
lipid
oligosaccharide
pyruvic acid
nonhuman
priority journal
rhizobium trifolii
Acetylation
Carbohydrate Sequence
Chromatography, Gel
Electrophoresis, Paper
Lipids
Oligosaccharides
Pyruvates
Pyruvic Acid
Rhizobium
Support, Non-U.S. Gov't
description EDTA-treated Rhizobium trifolii cells (strain NA30) incorporate radioactivity from (14C) labeled UDP-Clc, UDP-ClcA, Acetyl-Coa and/or phosphoenol pyruvate into chloroform:methanol:water (1:2:0.3) extracts. The incorporation products have properties of prenyl-phospho-sugars; mild alkaline hydrolysis of these extracts produce cyclic phosphate esters suggesting the presence of a diphosphate bridge, and mild acid or catalytic reduction-alkaline phosphatase treatments release four main componenets a, b, c and d, as judged by paper electrophoresis and chromatography and gel filtration studies. The four components can be obtained (14C)acetyl-labeled, but only compound c and to a lesser degree compound b can be (14C)pyruvate-labeled. For the exopolysaccharide produced by this strain the following repeating unit has been proposed (Robertsen et al. (1981), Plant Physiol. 67, 389-400): {A figure is presented}. The results obtained suggest that the octasaccharide repeating unit (compound a) with one (compound b) or two (compound c) ketal pyruvate residues are assembled on a lipid acceptor. All these compounds are assumed to be intermediates in the biosynthesis of R. trifolii exopolysaccharide. © 1986.
format JOUR
author Bossio, J.C.
Inon de Iannino, N.
Dankert, M.A.
author_facet Bossio, J.C.
Inon de Iannino, N.
Dankert, M.A.
author_sort Bossio, J.C.
title In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_short In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_full In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_fullStr In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_full_unstemmed In vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in Rhizobium trifolii
title_sort in vitro synthesis of a lipid-linked acetylated and pyruvilated olicosaccharide in rhizobium trifolii
url http://hdl.handle.net/20.500.12110/paper_0006291X_v134_n1_p205_Bossio
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AT inondeianninon invitrosynthesisofalipidlinkedacetylatedandpyruvilatedolicosaccharideinrhizobiumtrifolii
AT dankertma invitrosynthesisofalipidlinkedacetylatedandpyruvilatedolicosaccharideinrhizobiumtrifolii
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