Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens

In the present paper we report evidence about the formation of polyprenyl-phosphate monosaccharides, their elongation products and the assembly of dolichyl-diphosphate-oligosaccharide to endogenous T47D clon 11 proteins upon incubation with [14C]glucose. The influence of estradiol and two nonesteroi...

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Autores principales:	Santa Cruz, A.R., Medrano, E.E., Baldi, A.
Formato:	JOUR
Materias:	antiestrogen dolichol phosphate estradiol estrogen nafoxidine radioisotope tamoxifen breast breast cancer cancer cell glucosamine h 3 glucose c 14 human human cell priority journal Breast Neoplasms Cell Line Chromatography, Ion Exchange Chromatography, Thin Layer Estradiol Female Human Nafoxidine Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Sugars Pyrrolidines Support, Non-U.S. Gov't Tamoxifen
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0006291X_v126_n1_p382_SantaCruz
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_0006291X_v126_n1_p382_SantaCruz2023-10-03T14:03:37Z Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens Santa Cruz, A.R. Medrano, E.E. Baldi, A. antiestrogen dolichol phosphate estradiol estrogen nafoxidine radioisotope tamoxifen breast breast cancer cancer cell glucosamine h 3 glucose c 14 human human cell priority journal Breast Neoplasms Cell Line Chromatography, Ion Exchange Chromatography, Thin Layer Estradiol Female Human Nafoxidine Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Sugars Pyrrolidines Support, Non-U.S. Gov't Tamoxifen In the present paper we report evidence about the formation of polyprenyl-phosphate monosaccharides, their elongation products and the assembly of dolichyl-diphosphate-oligosaccharide to endogenous T47D clon 11 proteins upon incubation with [14C]glucose. The influence of estradiol and two nonesteroidal antiestrogens -nafoxidine and tamoxifen- was examined on the dolichol pathway in T47D cell cultures. Estradiol (1 nM) does not change the rate of synthesis of dolichyl-phosphate-sugar derivatives in contrast to nafoxidine and tamoxifen both a micromolar concentration, which induce a remarkable decrease in the formation of dolichol-sugar derivatives. In addition, T47D cells were pretreated with nafoxidine or tamoxifen during one hour, fresh medium supplemented with estradiol was added to the cells simultaneously with [14C]glucose. Results indicated that estradiol after nafoxidine induces a slight increase in the polyprenyl-sugar derivatives formation, however, estradiol after tamoxifen decreases the synthesis of these substances. © 1985. Fil:Santa Cruz, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0006291X_v126_n1_p382_SantaCruz
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	antiestrogen dolichol phosphate estradiol estrogen nafoxidine radioisotope tamoxifen breast breast cancer cancer cell glucosamine h 3 glucose c 14 human human cell priority journal Breast Neoplasms Cell Line Chromatography, Ion Exchange Chromatography, Thin Layer Estradiol Female Human Nafoxidine Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Sugars Pyrrolidines Support, Non-U.S. Gov't Tamoxifen
spellingShingle	antiestrogen dolichol phosphate estradiol estrogen nafoxidine radioisotope tamoxifen breast breast cancer cancer cell glucosamine h 3 glucose c 14 human human cell priority journal Breast Neoplasms Cell Line Chromatography, Ion Exchange Chromatography, Thin Layer Estradiol Female Human Nafoxidine Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Sugars Pyrrolidines Support, Non-U.S. Gov't Tamoxifen Santa Cruz, A.R. Medrano, E.E. Baldi, A. Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens
topic_facet	antiestrogen dolichol phosphate estradiol estrogen nafoxidine radioisotope tamoxifen breast breast cancer cancer cell glucosamine h 3 glucose c 14 human human cell priority journal Breast Neoplasms Cell Line Chromatography, Ion Exchange Chromatography, Thin Layer Estradiol Female Human Nafoxidine Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Sugars Pyrrolidines Support, Non-U.S. Gov't Tamoxifen
description	In the present paper we report evidence about the formation of polyprenyl-phosphate monosaccharides, their elongation products and the assembly of dolichyl-diphosphate-oligosaccharide to endogenous T47D clon 11 proteins upon incubation with [14C]glucose. The influence of estradiol and two nonesteroidal antiestrogens -nafoxidine and tamoxifen- was examined on the dolichol pathway in T47D cell cultures. Estradiol (1 nM) does not change the rate of synthesis of dolichyl-phosphate-sugar derivatives in contrast to nafoxidine and tamoxifen both a micromolar concentration, which induce a remarkable decrease in the formation of dolichol-sugar derivatives. In addition, T47D cells were pretreated with nafoxidine or tamoxifen during one hour, fresh medium supplemented with estradiol was added to the cells simultaneously with [14C]glucose. Results indicated that estradiol after nafoxidine induces a slight increase in the polyprenyl-sugar derivatives formation, however, estradiol after tamoxifen decreases the synthesis of these substances. © 1985.
format	JOUR
author	Santa Cruz, A.R. Medrano, E.E. Baldi, A.
author_facet	Santa Cruz, A.R. Medrano, E.E. Baldi, A.
author_sort	Santa Cruz, A.R.
title	Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens
title_short	Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens
title_full	Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens
title_fullStr	Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens
title_full_unstemmed	Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens
title_sort	dolichol-sugar derivative synthesis in human breast cancer cell line (t47d). effects of estrogens and antiestrogens
url	http://hdl.handle.net/20.500.12110/paper_0006291X_v126_n1_p382_SantaCruz
work_keys_str_mv	AT santacruzar dolicholsugarderivativesynthesisinhumanbreastcancercelllinet47deffectsofestrogensandantiestrogens AT medranoee dolicholsugarderivativesynthesisinhumanbreastcancercelllinet47deffectsofestrogensandantiestrogens AT baldia dolicholsugarderivativesynthesisinhumanbreastcancercelllinet47deffectsofestrogensandantiestrogens
_version_	1807321460956463104

Dolichol-sugar derivative synthesis in human breast cancer cell line (T47D). Effects of estrogens and antiestrogens

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