Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles
A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC-cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDS...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00052736_v1758_n1_p65_Ohtake |
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todo:paper_00052736_v1758_n1_p65_Ohtake2023-10-03T14:03:16Z Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles Ohtake, S. Schebor, C. de Pablo, J.J. Cholesterol DPPC Liposome Trehalose cholesterol crystallin dipalmitoylphosphatidylcholine liposome phospholipid trehalose article concentration (parameters) dehydration drug delivery system freeze drying gel hydration liquid priority journal protein interaction temperature 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cell Membrane Cholesterol Drug Delivery Systems Freeze Drying Lipid Bilayers Phase Transition Temperature Trehalose Water A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC-cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDSC and DSC are used to examine the phase behavior of hydrated and dehydrated vesicles, respectively. The concentration of trehalose inside and outside the vesicles is manipulated, and is shown to affect the relative stability of the membranes. Our results show for the first time that a combination of high inner and low outer trehalose concentration is able to decrease the gel-to-liquid crystalline phase temperature (Tm), while any other combination will not. Upon dehydration, the Tm of all lipid mixtures increases. The extent of the increase depends on the trehalose distribution across the bilayer. The Tm changes in the same direction with trehalose concentration for both freeze-dried and fully hydrated samples, suggesting that the trehalose distribution across the vesicle membrane, as well as the trehalose-phospholipid interaction, is maintained upon lyophilization. The results presented in this work may aid in the formulation of systems to be used in the lyophilization of liposomes for drug delivery applications. © 2006 Elsevier B.V. All rights reserved. Fil:Schebor, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00052736_v1758_n1_p65_Ohtake |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cholesterol DPPC Liposome Trehalose cholesterol crystallin dipalmitoylphosphatidylcholine liposome phospholipid trehalose article concentration (parameters) dehydration drug delivery system freeze drying gel hydration liquid priority journal protein interaction temperature 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cell Membrane Cholesterol Drug Delivery Systems Freeze Drying Lipid Bilayers Phase Transition Temperature Trehalose Water |
| spellingShingle |
Cholesterol DPPC Liposome Trehalose cholesterol crystallin dipalmitoylphosphatidylcholine liposome phospholipid trehalose article concentration (parameters) dehydration drug delivery system freeze drying gel hydration liquid priority journal protein interaction temperature 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cell Membrane Cholesterol Drug Delivery Systems Freeze Drying Lipid Bilayers Phase Transition Temperature Trehalose Water Ohtake, S. Schebor, C. de Pablo, J.J. Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles |
| topic_facet |
Cholesterol DPPC Liposome Trehalose cholesterol crystallin dipalmitoylphosphatidylcholine liposome phospholipid trehalose article concentration (parameters) dehydration drug delivery system freeze drying gel hydration liquid priority journal protein interaction temperature 1,2-Dipalmitoylphosphatidylcholine Calorimetry, Differential Scanning Cell Membrane Cholesterol Drug Delivery Systems Freeze Drying Lipid Bilayers Phase Transition Temperature Trehalose Water |
| description |
A systematic study is presented of the effects of trehalose on the physical properties of extruded DPPC-cholesterol unilamellar vesicles. Particular emphasis is placed on examining how the interactions present in the hydrated state translate into those in the dehydrated state. Observations from HSDSC and DSC are used to examine the phase behavior of hydrated and dehydrated vesicles, respectively. The concentration of trehalose inside and outside the vesicles is manipulated, and is shown to affect the relative stability of the membranes. Our results show for the first time that a combination of high inner and low outer trehalose concentration is able to decrease the gel-to-liquid crystalline phase temperature (Tm), while any other combination will not. Upon dehydration, the Tm of all lipid mixtures increases. The extent of the increase depends on the trehalose distribution across the bilayer. The Tm changes in the same direction with trehalose concentration for both freeze-dried and fully hydrated samples, suggesting that the trehalose distribution across the vesicle membrane, as well as the trehalose-phospholipid interaction, is maintained upon lyophilization. The results presented in this work may aid in the formulation of systems to be used in the lyophilization of liposomes for drug delivery applications. © 2006 Elsevier B.V. All rights reserved. |
| format |
JOUR |
| author |
Ohtake, S. Schebor, C. de Pablo, J.J. |
| author_facet |
Ohtake, S. Schebor, C. de Pablo, J.J. |
| author_sort |
Ohtake, S. |
| title |
Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles |
| title_short |
Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles |
| title_full |
Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles |
| title_fullStr |
Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles |
| title_full_unstemmed |
Effects of trehalose on the phase behavior of DPPC-cholesterol unilamellar vesicles |
| title_sort |
effects of trehalose on the phase behavior of dppc-cholesterol unilamellar vesicles |
| url |
http://hdl.handle.net/20.500.12110/paper_00052736_v1758_n1_p65_Ohtake |
| work_keys_str_mv |
AT ohtakes effectsoftrehaloseonthephasebehaviorofdppccholesterolunilamellarvesicles AT scheborc effectsoftrehaloseonthephasebehaviorofdppccholesterolunilamellarvesicles AT depablojj effectsoftrehaloseonthephasebehaviorofdppccholesterolunilamellarvesicles |
| _version_ |
1807316187863842816 |