Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein

There is evidence that anaemia is associated with aluminium (Al). We have already reported on the sensitivity to Al, showed by erythroid cell populations of animals chronically exposed to the metal. In order to investigate whether Al could also affect human cells, experiments were carried out both o...

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Autores principales:	Vittori, D., Garbossa, G., Lafourcade, C., Pérez, G., Nesse, A.
Formato:	JOUR
Materias:	Aluminium toxicity Anaemia Band 3 Erythrocyte morphology Membrane proteins aluminum dodecyl sulfate sodium erythrocyte band 3 protein erythropoietin anemia article cell culture cell maturation cell metabolism cell stimulation colony forming unit controlled study culture medium erythrocyte structure erythroid cell erythropoiesis human human cell immunoblotting polyacrylamide gel electrophoresis priority journal protein analysis protein degradation scanning electron microscopy stem cell Aluminum Compounds Anion Exchange Protein 1, Erythrocyte Cytosol Erythrocyte Aging Erythrocyte Membrane Erythrocytes Erythroid Progenitor Cells Humans Immunoblotting Time Factors Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00052736_v1558_n2_p142_Vittori
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00052736_v1558_n2_p142_Vittori
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spelling	todo:paper_00052736_v1558_n2_p142_Vittori2023-10-03T14:03:15Z Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein Vittori, D. Garbossa, G. Lafourcade, C. Pérez, G. Nesse, A. Aluminium toxicity Anaemia Band 3 Erythrocyte morphology Membrane proteins aluminum dodecyl sulfate sodium erythrocyte band 3 protein erythropoietin anemia article cell culture cell maturation cell metabolism cell stimulation colony forming unit controlled study culture medium erythrocyte structure erythroid cell erythropoiesis human human cell immunoblotting polyacrylamide gel electrophoresis priority journal protein analysis protein degradation scanning electron microscopy stem cell Aluminum Compounds Anion Exchange Protein 1, Erythrocyte Cytosol Erythrocyte Aging Erythrocyte Membrane Erythrocytes Erythroid Progenitor Cells Humans Immunoblotting Time Factors Animalia There is evidence that anaemia is associated with aluminium (Al). We have already reported on the sensitivity to Al, showed by erythroid cell populations of animals chronically exposed to the metal. In order to investigate whether Al could also affect human cells, experiments were carried out both on immature and mature human erythroid cells. Erythroid progenitors (CFU-E, colony-forming units-erythroid) concentrated from human peripheral blood were cultured in an Al-rich medium under erythropoietin stimulation and their development analysed. Human peripheral erythrocytes were aged in the presence of Al. Cells were examined using scanning electron microscopy, and membrane proteins analysed by polyacrylamide gel electrophoresis with sodium dodecyl sulphate and immunoblotting. The development of the Al-treated progenitors was 8750/6600-9200 CFU-E/10 6 cells, a significantly lower median value (P<0.05) than that showed by non-treated cells (12 300/11 200-20 700 CFU-E/10 6 cells). Erythrocyte morphological changes were induced by Al during the in vitro ageing. The cells lost their typical biconcave shape, turning into acanthocytes and stomatocytes. Simultaneously, an increased membrane protein breakdown compatible with band 3 degradation was detected. Besides, Al was found within the cells and attached to the membrane. The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors. © 2002 Elsevier Science B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00052736_v1558_n2_p142_Vittori
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Aluminium toxicity Anaemia Band 3 Erythrocyte morphology Membrane proteins aluminum dodecyl sulfate sodium erythrocyte band 3 protein erythropoietin anemia article cell culture cell maturation cell metabolism cell stimulation colony forming unit controlled study culture medium erythrocyte structure erythroid cell erythropoiesis human human cell immunoblotting polyacrylamide gel electrophoresis priority journal protein analysis protein degradation scanning electron microscopy stem cell Aluminum Compounds Anion Exchange Protein 1, Erythrocyte Cytosol Erythrocyte Aging Erythrocyte Membrane Erythrocytes Erythroid Progenitor Cells Humans Immunoblotting Time Factors Animalia
spellingShingle	Aluminium toxicity Anaemia Band 3 Erythrocyte morphology Membrane proteins aluminum dodecyl sulfate sodium erythrocyte band 3 protein erythropoietin anemia article cell culture cell maturation cell metabolism cell stimulation colony forming unit controlled study culture medium erythrocyte structure erythroid cell erythropoiesis human human cell immunoblotting polyacrylamide gel electrophoresis priority journal protein analysis protein degradation scanning electron microscopy stem cell Aluminum Compounds Anion Exchange Protein 1, Erythrocyte Cytosol Erythrocyte Aging Erythrocyte Membrane Erythrocytes Erythroid Progenitor Cells Humans Immunoblotting Time Factors Animalia Vittori, D. Garbossa, G. Lafourcade, C. Pérez, G. Nesse, A. Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein
topic_facet	Aluminium toxicity Anaemia Band 3 Erythrocyte morphology Membrane proteins aluminum dodecyl sulfate sodium erythrocyte band 3 protein erythropoietin anemia article cell culture cell maturation cell metabolism cell stimulation colony forming unit controlled study culture medium erythrocyte structure erythroid cell erythropoiesis human human cell immunoblotting polyacrylamide gel electrophoresis priority journal protein analysis protein degradation scanning electron microscopy stem cell Aluminum Compounds Anion Exchange Protein 1, Erythrocyte Cytosol Erythrocyte Aging Erythrocyte Membrane Erythrocytes Erythroid Progenitor Cells Humans Immunoblotting Time Factors Animalia
description	There is evidence that anaemia is associated with aluminium (Al). We have already reported on the sensitivity to Al, showed by erythroid cell populations of animals chronically exposed to the metal. In order to investigate whether Al could also affect human cells, experiments were carried out both on immature and mature human erythroid cells. Erythroid progenitors (CFU-E, colony-forming units-erythroid) concentrated from human peripheral blood were cultured in an Al-rich medium under erythropoietin stimulation and their development analysed. Human peripheral erythrocytes were aged in the presence of Al. Cells were examined using scanning electron microscopy, and membrane proteins analysed by polyacrylamide gel electrophoresis with sodium dodecyl sulphate and immunoblotting. The development of the Al-treated progenitors was 8750/6600-9200 CFU-E/10 6 cells, a significantly lower median value (P<0.05) than that showed by non-treated cells (12 300/11 200-20 700 CFU-E/10 6 cells). Erythrocyte morphological changes were induced by Al during the in vitro ageing. The cells lost their typical biconcave shape, turning into acanthocytes and stomatocytes. Simultaneously, an increased membrane protein breakdown compatible with band 3 degradation was detected. Besides, Al was found within the cells and attached to the membrane. The present in vitro results suggest that Al may disturb human erythropoiesis through combined effects on mature erythrocytes and cellular metabolism in late erythroid progenitors. © 2002 Elsevier Science B.V. All rights reserved.
format	JOUR
author	Vittori, D. Garbossa, G. Lafourcade, C. Pérez, G. Nesse, A.
author_facet	Vittori, D. Garbossa, G. Lafourcade, C. Pérez, G. Nesse, A.
author_sort	Vittori, D.
title	Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein
title_short	Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein
title_full	Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein
title_fullStr	Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein
title_full_unstemmed	Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein
title_sort	human erythroid cells are affected by aluminium. alteration of membrane band 3 protein
url	http://hdl.handle.net/20.500.12110/paper_00052736_v1558_n2_p142_Vittori
work_keys_str_mv	AT vittorid humanerythroidcellsareaffectedbyaluminiumalterationofmembraneband3protein AT garbossag humanerythroidcellsareaffectedbyaluminiumalterationofmembraneband3protein AT lafourcadec humanerythroidcellsareaffectedbyaluminiumalterationofmembraneband3protein AT perezg humanerythroidcellsareaffectedbyaluminiumalterationofmembraneband3protein AT nessea humanerythroidcellsareaffectedbyaluminiumalterationofmembraneband3protein
_version_	1807318867902464000

Human erythroid cells are affected by aluminium. Alteration of membrane band 3 protein

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