In vitro effect of a new cinnamic acid derivative against the epimastigote form of Trypanosoma cruzi

An intensive effort has been directed toward finding alternative drugs for treatment of Chagas' disease, caused by Trypanosoma cruzi (T. cruzi), and prophylaxis of blood in endemic areas. The preparation and in vitro evaluation as potential anti-protozoal agent of (2E)-N-(1,3-benzothiazol-2-yl)...

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Autores principales: Andreu, G.L.P., Inada, N.M., Pellón, R.F., Docampo, M.L., Fascio, M.L., D'Accorso, N.B., Vercesi, A.E.
Formato: JOUR
Materias:
Anti-protozoal agents
Chagas' disease
Cinnamic acid
Epimastigotes
Mitochondria
Oxidative stress
Trypanosoma cruzi
cad 1
cinnamic acid derivative
n (1,3 benzothiazol 2 yl) 3 (2,5 dimethoxyphenyl) 2 propenamide
reactive oxygen metabolite
unclassified drug
antiprotozoal activity
article
drug mechanism
drug structure
epimastigote
in vitro study
mitochondrial membrane potential
nonhuman
Amides
Animals
Annexin A5
Benzothiazoles
Cinnamates
Coloring Agents
Enzyme Inhibitors
Flow Cytometry
Indicators and Reagents
Membrane Potentials
Propidium
Reactive Oxygen Species
Trypanocidal Agents
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00044172_v59_n4_p207_Andreu
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00044172_v59_n4_p207_Andreu
record_format dspace
spelling todo:paper_00044172_v59_n4_p207_Andreu2023-10-03T13:57:13Z In vitro effect of a new cinnamic acid derivative against the epimastigote form of Trypanosoma cruzi Andreu, G.L.P. Inada, N.M. Pellón, R.F. Docampo, M.L. Fascio, M.L. D'Accorso, N.B. Vercesi, A.E. Anti-protozoal agents Chagas' disease Cinnamic acid Epimastigotes Mitochondria Oxidative stress Trypanosoma cruzi cad 1 cinnamic acid derivative n (1,3 benzothiazol 2 yl) 3 (2,5 dimethoxyphenyl) 2 propenamide reactive oxygen metabolite unclassified drug antiprotozoal activity article drug mechanism drug structure epimastigote in vitro study mitochondrial membrane potential nonhuman Trypanosoma cruzi Amides Animals Annexin A5 Benzothiazoles Cinnamates Coloring Agents Enzyme Inhibitors Flow Cytometry Indicators and Reagents Membrane Potentials Propidium Reactive Oxygen Species Trypanocidal Agents Trypanosoma cruzi An intensive effort has been directed toward finding alternative drugs for treatment of Chagas' disease, caused by Trypanosoma cruzi (T. cruzi), and prophylaxis of blood in endemic areas. The preparation and in vitro evaluation as potential anti-protozoal agent of (2E)-N-(1,3-benzothiazol-2-yl)-3-(2,5- dimethoxyphenyl)-2-propenamide (CAD-1) is presented. The results show that 0.05 mMCAD-1 induced 58.1% of T. cruzi epimastigotes death; mainly by apoptosis. The diminution in the transmembrane mitochondrial electrical potential together with the increase in the intracellular generation/accumulation of reactive oxygen species, suggest the parasites mitochondria as the main target for CAD-1-induced death. The concentration of 0.05 mM CAD-1 is not low enough to consider it as a potent tripanocydal agent. However the novel mechanismthat induce s T. cruzi death, together with the novelty of its chemical structure, point out CAD-1 as a head group compound that could serve as a template to obtain new, more potent anti-Chagas disease agents. © ECV Editio Cantor Verlag. Fil:Fascio, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:D'Accorso, N.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00044172_v59_n4_p207_Andreu
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Anti-protozoal agents
Chagas' disease
Cinnamic acid
Epimastigotes
Mitochondria
Oxidative stress
Trypanosoma cruzi
cad 1
cinnamic acid derivative
n (1,3 benzothiazol 2 yl) 3 (2,5 dimethoxyphenyl) 2 propenamide
reactive oxygen metabolite
unclassified drug
antiprotozoal activity
article
drug mechanism
drug structure
epimastigote
in vitro study
mitochondrial membrane potential
nonhuman
Trypanosoma cruzi
Amides
Animals
Annexin A5
Benzothiazoles
Cinnamates
Coloring Agents
Enzyme Inhibitors
Flow Cytometry
Indicators and Reagents
Membrane Potentials
Propidium
Reactive Oxygen Species
Trypanocidal Agents
Trypanosoma cruzi
spellingShingle Anti-protozoal agents
Chagas' disease
Cinnamic acid
Epimastigotes
Mitochondria
Oxidative stress
Trypanosoma cruzi
cad 1
cinnamic acid derivative
n (1,3 benzothiazol 2 yl) 3 (2,5 dimethoxyphenyl) 2 propenamide
reactive oxygen metabolite
unclassified drug
antiprotozoal activity
article
drug mechanism
drug structure
epimastigote
in vitro study
mitochondrial membrane potential
nonhuman
Trypanosoma cruzi
Amides
Animals
Annexin A5
Benzothiazoles
Cinnamates
Coloring Agents
Enzyme Inhibitors
Flow Cytometry
Indicators and Reagents
Membrane Potentials
Propidium
Reactive Oxygen Species
Trypanocidal Agents
Trypanosoma cruzi
Andreu, G.L.P.
Inada, N.M.
Pellón, R.F.
Docampo, M.L.
Fascio, M.L.
D'Accorso, N.B.
Vercesi, A.E.
In vitro effect of a new cinnamic acid derivative against the epimastigote form of Trypanosoma cruzi
topic_facet Anti-protozoal agents
Chagas' disease
Cinnamic acid
Epimastigotes
Mitochondria
Oxidative stress
Trypanosoma cruzi
cad 1
cinnamic acid derivative
n (1,3 benzothiazol 2 yl) 3 (2,5 dimethoxyphenyl) 2 propenamide
reactive oxygen metabolite
unclassified drug
antiprotozoal activity
article
drug mechanism
drug structure
epimastigote
in vitro study
mitochondrial membrane potential
nonhuman
Trypanosoma cruzi
Amides
Animals
Annexin A5
Benzothiazoles
Cinnamates
Coloring Agents
Enzyme Inhibitors
Flow Cytometry
Indicators and Reagents
Membrane Potentials
Propidium
Reactive Oxygen Species
Trypanocidal Agents
Trypanosoma cruzi
description An intensive effort has been directed toward finding alternative drugs for treatment of Chagas' disease, caused by Trypanosoma cruzi (T. cruzi), and prophylaxis of blood in endemic areas. The preparation and in vitro evaluation as potential anti-protozoal agent of (2E)-N-(1,3-benzothiazol-2-yl)-3-(2,5- dimethoxyphenyl)-2-propenamide (CAD-1) is presented. The results show that 0.05 mMCAD-1 induced 58.1% of T. cruzi epimastigotes death; mainly by apoptosis. The diminution in the transmembrane mitochondrial electrical potential together with the increase in the intracellular generation/accumulation of reactive oxygen species, suggest the parasites mitochondria as the main target for CAD-1-induced death. The concentration of 0.05 mM CAD-1 is not low enough to consider it as a potent tripanocydal agent. However the novel mechanismthat induce s T. cruzi death, together with the novelty of its chemical structure, point out CAD-1 as a head group compound that could serve as a template to obtain new, more potent anti-Chagas disease agents. © ECV Editio Cantor Verlag.
format JOUR
author Andreu, G.L.P.
Inada, N.M.
Pellón, R.F.
Docampo, M.L.
Fascio, M.L.
D'Accorso, N.B.
Vercesi, A.E.
author_facet Andreu, G.L.P.
Inada, N.M.
Pellón, R.F.
Docampo, M.L.
Fascio, M.L.
D'Accorso, N.B.
Vercesi, A.E.
author_sort Andreu, G.L.P.
title In vitro effect of a new cinnamic acid derivative against the epimastigote form of Trypanosoma cruzi
title_short In vitro effect of a new cinnamic acid derivative against the epimastigote form of Trypanosoma cruzi
title_full In vitro effect of a new cinnamic acid derivative against the epimastigote form of Trypanosoma cruzi
title_fullStr In vitro effect of a new cinnamic acid derivative against the epimastigote form of Trypanosoma cruzi
title_full_unstemmed In vitro effect of a new cinnamic acid derivative against the epimastigote form of Trypanosoma cruzi
title_sort in vitro effect of a new cinnamic acid derivative against the epimastigote form of trypanosoma cruzi
url http://hdl.handle.net/20.500.12110/paper_00044172_v59_n4_p207_Andreu
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