Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity

Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele ("crisp") exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not fo...

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Autores principales: Flawiá, M.M., Terenzi, H.F., Torres, H.N.
Formato: JOUR
Materias:
adenylate cyclase
enzyme deficiency
in vitro study
microorganism
mutant
neurospora crassa
theoretical study
Adenylate Cyclase
Crosses, Genetic
Membranes
Mutation
Neurospora
Neurospora crassa
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p334_Flawia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00039861_v180_n2_p334_Flawia2023-10-03T13:56:53Z Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity Flawiá, M.M. Terenzi, H.F. Torres, H.N. adenylate cyclase enzyme deficiency in vitro study microorganism mutant neurospora crassa theoretical study Adenylate Cyclase Crosses, Genetic Membranes Mutation Neurospora Neurospora crassa Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele ("crisp") exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not for the "crisp" morphology and recessive in heterocaryons carrying mutated and wild-type cr-1 alleles. The reduced adenylate cyclase activity detected in extracts from cr-1 mutants was not due to an impairment in the extraction of membranes, to a preferential inactivation of the enzyme after extraction, or to the presence of inhibitors. © 1977. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p334_Flawia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic adenylate cyclase
enzyme deficiency
in vitro study
microorganism
mutant
neurospora crassa
theoretical study
Adenylate Cyclase
Crosses, Genetic
Membranes
Mutation
Neurospora
Neurospora crassa
spellingShingle adenylate cyclase
enzyme deficiency
in vitro study
microorganism
mutant
neurospora crassa
theoretical study
Adenylate Cyclase
Crosses, Genetic
Membranes
Mutation
Neurospora
Neurospora crassa
Flawiá, M.M.
Terenzi, H.F.
Torres, H.N.
Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity
topic_facet adenylate cyclase
enzyme deficiency
in vitro study
microorganism
mutant
neurospora crassa
theoretical study
Adenylate Cyclase
Crosses, Genetic
Membranes
Mutation
Neurospora
Neurospora crassa
description Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele ("crisp") exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not for the "crisp" morphology and recessive in heterocaryons carrying mutated and wild-type cr-1 alleles. The reduced adenylate cyclase activity detected in extracts from cr-1 mutants was not due to an impairment in the extraction of membranes, to a preferential inactivation of the enzyme after extraction, or to the presence of inhibitors. © 1977.
format JOUR
author Flawiá, M.M.
Terenzi, H.F.
Torres, H.N.
author_facet Flawiá, M.M.
Terenzi, H.F.
Torres, H.N.
author_sort Flawiá, M.M.
title Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity
title_short Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity
title_full Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity
title_fullStr Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity
title_full_unstemmed Characterization of Neurospora crassa mutant strains deficient in adenylate cyclase activity
title_sort characterization of neurospora crassa mutant strains deficient in adenylate cyclase activity
url http://hdl.handle.net/20.500.12110/paper_00039861_v180_n2_p334_Flawia
work_keys_str_mv AT flawiamm characterizationofneurosporacrassamutantstrainsdeficientinadenylatecyclaseactivity
AT terenzihf characterizationofneurosporacrassamutantstrainsdeficientinadenylatecyclaseactivity
AT torreshn characterizationofneurosporacrassamutantstrainsdeficientinadenylatecyclaseactivity
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