Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii
The germination of spores of Mucor rouxii into hyphae was inhibited by 2 mm dibutyryl cyclic adenosine 3′,5′-monophosphate or 7 mm cyclic adenosine 3′,5′-monophosphate; under these conditions spores developed into budding spherical cells instead of filaments, provided that glucose was present in the...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00039861_v169_n2_p449_Paveto |
| Aporte de: |
| id |
todo:paper_00039861_v169_n2_p449_Paveto |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00039861_v169_n2_p449_Paveto2023-10-03T13:56:51Z Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii Paveto, C. Epstein, A. Passeron, S. adenylate cyclase cyclic amp cyclic amp phosphodiesterase fungus fungus spore in vitro study microorganism spore germination theoretical study 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Bucladesine Cyclic AMP Mucor Phosphoric Diester Hydrolases Subcellular Fractions Time Factors The germination of spores of Mucor rouxii into hyphae was inhibited by 2 mm dibutyryl cyclic adenosine 3′,5′-monophosphate or 7 mm cyclic adenosine 3′,5′-monophosphate; under these conditions spores developed into budding spherical cells instead of filaments, provided that glucose was present in the culture medium. Removal of the cyclic nucleotides resulted in the conversion of yeast cells into hyphae. Dibutyryl cyclic adenosine 3′,5′-monophosphate (2 mm) also inhibited the transformation of yeast to mycelia after exposure of yeast culture to air. Since in all living systems so far studied adenylate cyclase and cyclic adenosine 3′,5′-monophosphate phosphodiesterase are involved in maintaining the intracellular cyclic adenosine monophosphate level, the activity of both enzymes and the intracellular concentration of cyclic adenosine monophosphate were investigated in yeast and mycelium extracts. Cyclic adenosine monophosphate phosphodiesterase and adenylate cyclase activities could be demonstrated in extracts of M. rouxii. The specific activity of adenylate cyclase did not vary appreciably with the fungus morphology. On the contrary, cyclic adenosine monophosphate phosphodiesterase activity was four- to sixfold higher in mycelial extracts than in yeast extracts and reflected quite accurately the observed changes in intracellular cyclic adenosine monophosphate levels; these were three to four times higher in yeast cells than in mycelium. © 1975. Fil:Paveto, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00039861_v169_n2_p449_Paveto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenylate cyclase cyclic amp cyclic amp phosphodiesterase fungus fungus spore in vitro study microorganism spore germination theoretical study 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Bucladesine Cyclic AMP Mucor Phosphoric Diester Hydrolases Subcellular Fractions Time Factors |
| spellingShingle |
adenylate cyclase cyclic amp cyclic amp phosphodiesterase fungus fungus spore in vitro study microorganism spore germination theoretical study 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Bucladesine Cyclic AMP Mucor Phosphoric Diester Hydrolases Subcellular Fractions Time Factors Paveto, C. Epstein, A. Passeron, S. Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii |
| topic_facet |
adenylate cyclase cyclic amp cyclic amp phosphodiesterase fungus fungus spore in vitro study microorganism spore germination theoretical study 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Bucladesine Cyclic AMP Mucor Phosphoric Diester Hydrolases Subcellular Fractions Time Factors |
| description |
The germination of spores of Mucor rouxii into hyphae was inhibited by 2 mm dibutyryl cyclic adenosine 3′,5′-monophosphate or 7 mm cyclic adenosine 3′,5′-monophosphate; under these conditions spores developed into budding spherical cells instead of filaments, provided that glucose was present in the culture medium. Removal of the cyclic nucleotides resulted in the conversion of yeast cells into hyphae. Dibutyryl cyclic adenosine 3′,5′-monophosphate (2 mm) also inhibited the transformation of yeast to mycelia after exposure of yeast culture to air. Since in all living systems so far studied adenylate cyclase and cyclic adenosine 3′,5′-monophosphate phosphodiesterase are involved in maintaining the intracellular cyclic adenosine monophosphate level, the activity of both enzymes and the intracellular concentration of cyclic adenosine monophosphate were investigated in yeast and mycelium extracts. Cyclic adenosine monophosphate phosphodiesterase and adenylate cyclase activities could be demonstrated in extracts of M. rouxii. The specific activity of adenylate cyclase did not vary appreciably with the fungus morphology. On the contrary, cyclic adenosine monophosphate phosphodiesterase activity was four- to sixfold higher in mycelial extracts than in yeast extracts and reflected quite accurately the observed changes in intracellular cyclic adenosine monophosphate levels; these were three to four times higher in yeast cells than in mycelium. © 1975. |
| format |
JOUR |
| author |
Paveto, C. Epstein, A. Passeron, S. |
| author_facet |
Paveto, C. Epstein, A. Passeron, S. |
| author_sort |
Paveto, C. |
| title |
Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii |
| title_short |
Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii |
| title_full |
Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii |
| title_fullStr |
Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii |
| title_full_unstemmed |
Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii |
| title_sort |
studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus mucor rouxii |
| url |
http://hdl.handle.net/20.500.12110/paper_00039861_v169_n2_p449_Paveto |
| work_keys_str_mv |
AT pavetoc studiesoncyclicadenosine35monophosphatelevelsadenylatecyclaseandphosphodiesteraseactivitiesinthedimorphicfungusmucorrouxii AT epsteina studiesoncyclicadenosine35monophosphatelevelsadenylatecyclaseandphosphodiesteraseactivitiesinthedimorphicfungusmucorrouxii AT passerons studiesoncyclicadenosine35monophosphatelevelsadenylatecyclaseandphosphodiesteraseactivitiesinthedimorphicfungusmucorrouxii |
| _version_ |
1807320767007817728 |