Further studies on high molecular weight liver glycogen
A simple method for the extraction of undegraded liver glycogen is described. The molecular weight distribution curve as measured by gradient centrifugation was quite variable and was found to be unrelated to glycogen content. The concentration of malto-oligosaccharides in liver was estimated by a n...
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todo:paper_00039861_v113_n2_p265_Mordoh2023-10-03T13:56:47Z Further studies on high molecular weight liver glycogen Mordoh, J. Krisman, C.R. Leloir, L.F. guanidine derivative oligosaccharide sodium hydroxide urea animal article cell fractionation chemistry glycogen liver level heat in vitro study molecular weight rat ultrasound Animal Chemistry Guanidines Heat In Vitro Liver Glycogen Molecular Weight Oligosaccharides Rats Sodium Hydroxide Subcellular Fractions Ultrasonics Urea A simple method for the extraction of undegraded liver glycogen is described. The molecular weight distribution curve as measured by gradient centrifugation was quite variable and was found to be unrelated to glycogen content. The concentration of malto-oligosaccharides in liver was estimated by a new method and was found to remain constant under conditions in which glycogen content varied considerably. Native glycogen was compared with that prepared in vitro with purified enzymes. Although both preparations were similar in molecular weight and in their appearance under the electron microscope, they differed in the type of breakdown produced by acid, alkali, heat, and ultrasonic vibrations. When glycogen prepared in vitro was submitted to these procedures, its sedimentation coefficient decreased progressively, while native glycogen was broken down preferentially to a lighter population of about S = 100. © 1966. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00039861_v113_n2_p265_Mordoh |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
guanidine derivative oligosaccharide sodium hydroxide urea animal article cell fractionation chemistry glycogen liver level heat in vitro study molecular weight rat ultrasound Animal Chemistry Guanidines Heat In Vitro Liver Glycogen Molecular Weight Oligosaccharides Rats Sodium Hydroxide Subcellular Fractions Ultrasonics Urea |
| spellingShingle |
guanidine derivative oligosaccharide sodium hydroxide urea animal article cell fractionation chemistry glycogen liver level heat in vitro study molecular weight rat ultrasound Animal Chemistry Guanidines Heat In Vitro Liver Glycogen Molecular Weight Oligosaccharides Rats Sodium Hydroxide Subcellular Fractions Ultrasonics Urea Mordoh, J. Krisman, C.R. Leloir, L.F. Further studies on high molecular weight liver glycogen |
| topic_facet |
guanidine derivative oligosaccharide sodium hydroxide urea animal article cell fractionation chemistry glycogen liver level heat in vitro study molecular weight rat ultrasound Animal Chemistry Guanidines Heat In Vitro Liver Glycogen Molecular Weight Oligosaccharides Rats Sodium Hydroxide Subcellular Fractions Ultrasonics Urea |
| description |
A simple method for the extraction of undegraded liver glycogen is described. The molecular weight distribution curve as measured by gradient centrifugation was quite variable and was found to be unrelated to glycogen content. The concentration of malto-oligosaccharides in liver was estimated by a new method and was found to remain constant under conditions in which glycogen content varied considerably. Native glycogen was compared with that prepared in vitro with purified enzymes. Although both preparations were similar in molecular weight and in their appearance under the electron microscope, they differed in the type of breakdown produced by acid, alkali, heat, and ultrasonic vibrations. When glycogen prepared in vitro was submitted to these procedures, its sedimentation coefficient decreased progressively, while native glycogen was broken down preferentially to a lighter population of about S = 100. © 1966. |
| format |
JOUR |
| author |
Mordoh, J. Krisman, C.R. Leloir, L.F. |
| author_facet |
Mordoh, J. Krisman, C.R. Leloir, L.F. |
| author_sort |
Mordoh, J. |
| title |
Further studies on high molecular weight liver glycogen |
| title_short |
Further studies on high molecular weight liver glycogen |
| title_full |
Further studies on high molecular weight liver glycogen |
| title_fullStr |
Further studies on high molecular weight liver glycogen |
| title_full_unstemmed |
Further studies on high molecular weight liver glycogen |
| title_sort |
further studies on high molecular weight liver glycogen |
| url |
http://hdl.handle.net/20.500.12110/paper_00039861_v113_n2_p265_Mordoh |
| work_keys_str_mv |
AT mordohj furtherstudiesonhighmolecularweightliverglycogen AT krismancr furtherstudiesonhighmolecularweightliverglycogen AT leloirlf furtherstudiesonhighmolecularweightliverglycogen |
| _version_ |
1782025295986950144 |