Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events

A simplified methodology to acquire steady-state emission spectra and quantum yields of highly absorbing samples is presented. The experimental setup consists of a commercial spectrofluorometer adapted to transmission geometry, allowing the detection of the emitted light at 180° with respect to the...

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Autores principales: Krimer, N.I., Rodrigues, D., Rodríguez, H.B., Mirenda, M.
Formato: JOUR
Materias:
Emission spectroscopy
Quantum yield
Aggregation phenomena
Concentrated solution
Mathematical approach
Quantitative approach
Re-absorption corrections
Steady state fluorescences
Steady-state emissions
Transmission geometries
Quantum theory
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032700_v89_n1_p640_Krimer
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00032700_v89_n1_p640_Krimer
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spelling todo:paper_00032700_v89_n1_p640_Krimer2023-10-03T13:56:07Z Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events Krimer, N.I. Rodrigues, D. Rodríguez, H.B. Mirenda, M. Emission spectroscopy Quantum yield Aggregation phenomena Concentrated solution Mathematical approach Quantitative approach Re-absorption corrections Steady state fluorescences Steady-state emissions Transmission geometries Quantum theory A simplified methodology to acquire steady-state emission spectra and quantum yields of highly absorbing samples is presented. The experimental setup consists of a commercial spectrofluorometer adapted to transmission geometry, allowing the detection of the emitted light at 180° with respect to the excitation beam. The procedure includes two different mathematical approaches to describe and reproduce the distortions caused by reabsorption on emission spectra and quantum yields. Toluene solutions of 9,10-diphenylanthracence, DPA, with concentrations ranging between 1.12 × 10-5 and 1.30 × 10-2 M, were used to validate the proposed methodology. This dye has significant probability of reabsorption and re-emission in concentrated solutions without showing self-quenching or aggregation phenomena. The results indicate that the reabsorption corrections, applied on molecular emission spectra and quantum yields of the samples, accurately reproduce experimental data. A further discussion is performed concerning why the re-emitted radiation is not detected in the experiments, even at the highest DPA concentrations. © 2016 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032700_v89_n1_p640_Krimer
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Emission spectroscopy
Quantum yield
Aggregation phenomena
Concentrated solution
Mathematical approach
Quantitative approach
Re-absorption corrections
Steady state fluorescences
Steady-state emissions
Transmission geometries
Quantum theory
spellingShingle Emission spectroscopy
Quantum yield
Aggregation phenomena
Concentrated solution
Mathematical approach
Quantitative approach
Re-absorption corrections
Steady state fluorescences
Steady-state emissions
Transmission geometries
Quantum theory
Krimer, N.I.
Rodrigues, D.
Rodríguez, H.B.
Mirenda, M.
Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events
topic_facet Emission spectroscopy
Quantum yield
Aggregation phenomena
Concentrated solution
Mathematical approach
Quantitative approach
Re-absorption corrections
Steady state fluorescences
Steady-state emissions
Transmission geometries
Quantum theory
description A simplified methodology to acquire steady-state emission spectra and quantum yields of highly absorbing samples is presented. The experimental setup consists of a commercial spectrofluorometer adapted to transmission geometry, allowing the detection of the emitted light at 180° with respect to the excitation beam. The procedure includes two different mathematical approaches to describe and reproduce the distortions caused by reabsorption on emission spectra and quantum yields. Toluene solutions of 9,10-diphenylanthracence, DPA, with concentrations ranging between 1.12 × 10-5 and 1.30 × 10-2 M, were used to validate the proposed methodology. This dye has significant probability of reabsorption and re-emission in concentrated solutions without showing self-quenching or aggregation phenomena. The results indicate that the reabsorption corrections, applied on molecular emission spectra and quantum yields of the samples, accurately reproduce experimental data. A further discussion is performed concerning why the re-emitted radiation is not detected in the experiments, even at the highest DPA concentrations. © 2016 American Chemical Society.
format JOUR
author Krimer, N.I.
Rodrigues, D.
Rodríguez, H.B.
Mirenda, M.
author_facet Krimer, N.I.
Rodrigues, D.
Rodríguez, H.B.
Mirenda, M.
author_sort Krimer, N.I.
title Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events
title_short Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events
title_full Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events
title_fullStr Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events
title_full_unstemmed Steady-State Fluorescence of Highly Absorbing Samples in Transmission Geometry: A Simplified Quantitative Approach Considering Reabsorption Events
title_sort steady-state fluorescence of highly absorbing samples in transmission geometry: a simplified quantitative approach considering reabsorption events
url http://hdl.handle.net/20.500.12110/paper_00032700_v89_n1_p640_Krimer
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AT rodriguesd steadystatefluorescenceofhighlyabsorbingsamplesintransmissiongeometryasimplifiedquantitativeapproachconsideringreabsorptionevents
AT rodriguezhb steadystatefluorescenceofhighlyabsorbingsamplesintransmissiongeometryasimplifiedquantitativeapproachconsideringreabsorptionevents
AT mirendam steadystatefluorescenceofhighlyabsorbingsamplesintransmissiongeometryasimplifiedquantitativeapproachconsideringreabsorptionevents
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