1D and 2D temperature imaging with a fluorescent ruthenium complex

Temperature imaging based on the fluorescence of the complex [Ru(bpy) 3]2+ is described. The method allows precise temperature measurement on unidimensional flow injection reactors and bidimensional measurement on dishes for biological and biochemical assays. The fluorescence dependence on temperatu...

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Detalles Bibliográficos
Autores principales:	Filevich, O., Etchenique, R.
Formato:	JOUR
Materias:	Bioassay Cameras Chemical reactors Fluorescence Imaging techniques Temperature measurement Flow injection reactors Fluorescence microscopes Two-point calibration Ruthenium compounds ruthenium complex article bioassay calibration camera filter flow injection analysis fluorescence fluorescence microscope imaging linear system optical resolution optics temperature imaging temperature measurement
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00032700_v78_n21_p7499_Filevich
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00032700_v78_n21_p7499_Filevich
record_format	dspace
spelling	todo:paper_00032700_v78_n21_p7499_Filevich2023-10-03T13:56:01Z 1D and 2D temperature imaging with a fluorescent ruthenium complex Filevich, O. Etchenique, R. Bioassay Cameras Chemical reactors Fluorescence Imaging techniques Temperature measurement Flow injection reactors Fluorescence microscopes Two-point calibration Ruthenium compounds ruthenium complex article bioassay calibration camera filter flow injection analysis fluorescence fluorescence microscope imaging linear system optical resolution optics temperature imaging temperature measurement Temperature imaging based on the fluorescence of the complex [Ru(bpy) 3]2+ is described. The method allows precise temperature measurement on unidimensional flow injection reactors and bidimensional measurement on dishes for biological and biochemical assays. The fluorescence dependence on temperature is linear, achieving a resolution of 0.05 K with a simple two-point calibration. The large Stokes shift of [Ru(bpy) 3]2+ makes it easy to use a simple CCD camera without special filters. Large or small area fields can be achieved by changing the optics of the camera. High spatial resolution is possible by using any fluorescence microscope. © 2006 American Chemical Society. Fil:Filevich, O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Etchenique, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032700_v78_n21_p7499_Filevich
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bioassay Cameras Chemical reactors Fluorescence Imaging techniques Temperature measurement Flow injection reactors Fluorescence microscopes Two-point calibration Ruthenium compounds ruthenium complex article bioassay calibration camera filter flow injection analysis fluorescence fluorescence microscope imaging linear system optical resolution optics temperature imaging temperature measurement
spellingShingle	Bioassay Cameras Chemical reactors Fluorescence Imaging techniques Temperature measurement Flow injection reactors Fluorescence microscopes Two-point calibration Ruthenium compounds ruthenium complex article bioassay calibration camera filter flow injection analysis fluorescence fluorescence microscope imaging linear system optical resolution optics temperature imaging temperature measurement Filevich, O. Etchenique, R. 1D and 2D temperature imaging with a fluorescent ruthenium complex
topic_facet	Bioassay Cameras Chemical reactors Fluorescence Imaging techniques Temperature measurement Flow injection reactors Fluorescence microscopes Two-point calibration Ruthenium compounds ruthenium complex article bioassay calibration camera filter flow injection analysis fluorescence fluorescence microscope imaging linear system optical resolution optics temperature imaging temperature measurement
description	Temperature imaging based on the fluorescence of the complex [Ru(bpy) 3]2+ is described. The method allows precise temperature measurement on unidimensional flow injection reactors and bidimensional measurement on dishes for biological and biochemical assays. The fluorescence dependence on temperature is linear, achieving a resolution of 0.05 K with a simple two-point calibration. The large Stokes shift of [Ru(bpy) 3]2+ makes it easy to use a simple CCD camera without special filters. Large or small area fields can be achieved by changing the optics of the camera. High spatial resolution is possible by using any fluorescence microscope. © 2006 American Chemical Society.
format	JOUR
author	Filevich, O. Etchenique, R.
author_facet	Filevich, O. Etchenique, R.
author_sort	Filevich, O.
title	1D and 2D temperature imaging with a fluorescent ruthenium complex
title_short	1D and 2D temperature imaging with a fluorescent ruthenium complex
title_full	1D and 2D temperature imaging with a fluorescent ruthenium complex
title_fullStr	1D and 2D temperature imaging with a fluorescent ruthenium complex
title_full_unstemmed	1D and 2D temperature imaging with a fluorescent ruthenium complex
title_sort	1d and 2d temperature imaging with a fluorescent ruthenium complex
url	http://hdl.handle.net/20.500.12110/paper_00032700_v78_n21_p7499_Filevich
work_keys_str_mv	AT filevicho 1dand2dtemperatureimagingwithafluorescentrutheniumcomplex AT etcheniquer 1dand2dtemperatureimagingwithafluorescentrutheniumcomplex
_version_	1807316889223823360

1D and 2D temperature imaging with a fluorescent ruthenium complex

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