Study of layer-by-layer self-assembled viscoelastic films on thickness-shear mode resonator surfaces
We describe quartz crystal electroacoustic admittance studies in thickness shear mode resonators loaded with self-assembled multilayers composed of alternate layers of glucose oxidase (GOx) and poly(allylamine) covalently attached to [Os(bpy)2CIPyCOH]-, (PAH-Os), deposited on a 3-mercaptopropanesulf...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00032700_v74_n14_p3281_Calvo |
| Aporte de: |
| id |
todo:paper_00032700_v74_n14_p3281_Calvo |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00032700_v74_n14_p3281_Calvo2023-10-03T13:56:00Z Study of layer-by-layer self-assembled viscoelastic films on thickness-shear mode resonator surfaces Calvo, E.J. Forzani, E.S. Otero, M. Enzyme films Acoustic impedance Acoustoelectric effects Enzymes Multilayers Quartz Crystal resonators allylamine glucose oxidase gold metal complex osmium polymer sulfonic acid derivative acoustic impedance article crystal density electrochemistry film thickness viscoelasticity viscosity Allylamine Glucose Oxidase Osmium Compounds Polymers Sulfonic Acids We describe quartz crystal electroacoustic admittance studies in thickness shear mode resonators loaded with self-assembled multilayers composed of alternate layers of glucose oxidase (GOx) and poly(allylamine) covalently attached to [Os(bpy)2CIPyCOH]-, (PAH-Os), deposited on a 3-mercaptopropanesulfonic acid (MPS)-modified gold on the quartz crystal. The complex acoustic impedance parameters, RS and XLS of a lumped-element Butterworth-Van Dyke (BVD) resonator have been determined for organized thin films of different thickness obtained by varying the number of enzyme layers, n, in (PAH-Os)n(GOx)n structures. The ellipsometric film thickness and mass for dry enzyme multilayer films and films in contact with water were evaluated, and the average film density was estimated. By combination of the estimated film thickness and density, the expression for the surface mechanical impedance of the lumped-element modified resonator (Granstaff and Martin model), and the liquid density and viscosity, we simulate the layer-by-layer film growth on the basis of the measured electroacoustic impedance. The complex impedance XLS and RS increase with film thickness and the enzyme films can be regarded as acoustically thin in the reduced state for films thinner than 600 nm. We have also measured electroacoustic parameters for PAH-Os/GOx self-assembled multilayers under electrochemical perturbation in a buffer electrolytic solution. The electrostatically self-assembled multilayers behaved as lossy viscoelastic films at 10 MHz with G′f and G′ f on the order of 106 Pa. The films became viscoelastic upon oxidation to Os(III), resulting in an increase of RS and XLS in the oxidized state with the number of (PAHOs)(GOx) bilayers due to film swelling and an increase in the shear moduli during oxidation. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Otero, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032700_v74_n14_p3281_Calvo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Enzyme films Acoustic impedance Acoustoelectric effects Enzymes Multilayers Quartz Crystal resonators allylamine glucose oxidase gold metal complex osmium polymer sulfonic acid derivative acoustic impedance article crystal density electrochemistry film thickness viscoelasticity viscosity Allylamine Glucose Oxidase Osmium Compounds Polymers Sulfonic Acids |
| spellingShingle |
Enzyme films Acoustic impedance Acoustoelectric effects Enzymes Multilayers Quartz Crystal resonators allylamine glucose oxidase gold metal complex osmium polymer sulfonic acid derivative acoustic impedance article crystal density electrochemistry film thickness viscoelasticity viscosity Allylamine Glucose Oxidase Osmium Compounds Polymers Sulfonic Acids Calvo, E.J. Forzani, E.S. Otero, M. Study of layer-by-layer self-assembled viscoelastic films on thickness-shear mode resonator surfaces |
| topic_facet |
Enzyme films Acoustic impedance Acoustoelectric effects Enzymes Multilayers Quartz Crystal resonators allylamine glucose oxidase gold metal complex osmium polymer sulfonic acid derivative acoustic impedance article crystal density electrochemistry film thickness viscoelasticity viscosity Allylamine Glucose Oxidase Osmium Compounds Polymers Sulfonic Acids |
| description |
We describe quartz crystal electroacoustic admittance studies in thickness shear mode resonators loaded with self-assembled multilayers composed of alternate layers of glucose oxidase (GOx) and poly(allylamine) covalently attached to [Os(bpy)2CIPyCOH]-, (PAH-Os), deposited on a 3-mercaptopropanesulfonic acid (MPS)-modified gold on the quartz crystal. The complex acoustic impedance parameters, RS and XLS of a lumped-element Butterworth-Van Dyke (BVD) resonator have been determined for organized thin films of different thickness obtained by varying the number of enzyme layers, n, in (PAH-Os)n(GOx)n structures. The ellipsometric film thickness and mass for dry enzyme multilayer films and films in contact with water were evaluated, and the average film density was estimated. By combination of the estimated film thickness and density, the expression for the surface mechanical impedance of the lumped-element modified resonator (Granstaff and Martin model), and the liquid density and viscosity, we simulate the layer-by-layer film growth on the basis of the measured electroacoustic impedance. The complex impedance XLS and RS increase with film thickness and the enzyme films can be regarded as acoustically thin in the reduced state for films thinner than 600 nm. We have also measured electroacoustic parameters for PAH-Os/GOx self-assembled multilayers under electrochemical perturbation in a buffer electrolytic solution. The electrostatically self-assembled multilayers behaved as lossy viscoelastic films at 10 MHz with G′f and G′ f on the order of 106 Pa. The films became viscoelastic upon oxidation to Os(III), resulting in an increase of RS and XLS in the oxidized state with the number of (PAHOs)(GOx) bilayers due to film swelling and an increase in the shear moduli during oxidation. |
| format |
JOUR |
| author |
Calvo, E.J. Forzani, E.S. Otero, M. |
| author_facet |
Calvo, E.J. Forzani, E.S. Otero, M. |
| author_sort |
Calvo, E.J. |
| title |
Study of layer-by-layer self-assembled viscoelastic films on thickness-shear mode resonator surfaces |
| title_short |
Study of layer-by-layer self-assembled viscoelastic films on thickness-shear mode resonator surfaces |
| title_full |
Study of layer-by-layer self-assembled viscoelastic films on thickness-shear mode resonator surfaces |
| title_fullStr |
Study of layer-by-layer self-assembled viscoelastic films on thickness-shear mode resonator surfaces |
| title_full_unstemmed |
Study of layer-by-layer self-assembled viscoelastic films on thickness-shear mode resonator surfaces |
| title_sort |
study of layer-by-layer self-assembled viscoelastic films on thickness-shear mode resonator surfaces |
| url |
http://hdl.handle.net/20.500.12110/paper_00032700_v74_n14_p3281_Calvo |
| work_keys_str_mv |
AT calvoej studyoflayerbylayerselfassembledviscoelasticfilmsonthicknessshearmoderesonatorsurfaces AT forzanies studyoflayerbylayerselfassembledviscoelasticfilmsonthicknessshearmoderesonatorsurfaces AT oterom studyoflayerbylayerselfassembledviscoelasticfilmsonthicknessshearmoderesonatorsurfaces |
| _version_ |
1807314517989785600 |