Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode
The uptake of glucose oxidase (GOx) onto a polycationic redox polymer (PAA-Os)-modified surface, by adsorption from dilute aqueous GOx solutions, was followed by the quartz crystal microbalance (QCM) and shows double exponential kinetics. The electrochemistry of the layer-by-layer-deposited redox-ac...
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todo:paper_00032700_v73_n6_p1161_Calvo2023-10-03T13:56:00Z Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode Calvo, E.J. Etchenique, R. Pietrasanta, L. Wolosiuk, A. Danilowicz, C. Redox polymers Adsorption Agglomeration Cyclic voltammetry Electrochemistry Electrodes Glucose Gold Polymers Quartz Self assembly Enzymes 2,2' diaminoethyldisulfide alkane derivative beta glucose enzyme glucose glucose derivative glucose oxidase gold osmium derivative polyamine derivative polycation polymer silicon dioxide unclassified drug water adsorption aqueous solution article atomic force microscopy cyclic potentiometry electrochemistry electrode kinetics oxidation reduction reaction protein assembly Biosensing Techniques Electrochemistry Glucose Oxidase Organometallic Compounds Oxidation-Reduction The uptake of glucose oxidase (GOx) onto a polycationic redox polymer (PAA-Os)-modified surface, by adsorption from dilute aqueous GOx solutions, was followed by the quartz crystal microbalance (QCM) and shows double exponential kinetics. The electrochemistry of the layer-by-layer-deposited redox-active polymer was followed by cyclic voltammetry in glucose-free solutions, and the enzyme catalysis mediated by the redox polymer was studied in β-D-glucose-containing solutions. AFM studies of the different layers showed the existence of large two dimension enzyme aggregates on the osmium polymer for 1 μM GOx and less aggregation for 50 nM GOx solutions. When the short alkanethiol, 2,2′-diaminoethyldisulfide was preadsorbed onto gold, a monoexponential adsorption law was observed, and single GOx enzyme molecules could be seen on the surface where the enzyme was adsorbed from 50 nM GOx in water. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Etchenique, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032700_v73_n6_p1161_Calvo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Redox polymers Adsorption Agglomeration Cyclic voltammetry Electrochemistry Electrodes Glucose Gold Polymers Quartz Self assembly Enzymes 2,2' diaminoethyldisulfide alkane derivative beta glucose enzyme glucose glucose derivative glucose oxidase gold osmium derivative polyamine derivative polycation polymer silicon dioxide unclassified drug water adsorption aqueous solution article atomic force microscopy cyclic potentiometry electrochemistry electrode kinetics oxidation reduction reaction protein assembly Biosensing Techniques Electrochemistry Glucose Oxidase Organometallic Compounds Oxidation-Reduction |
| spellingShingle |
Redox polymers Adsorption Agglomeration Cyclic voltammetry Electrochemistry Electrodes Glucose Gold Polymers Quartz Self assembly Enzymes 2,2' diaminoethyldisulfide alkane derivative beta glucose enzyme glucose glucose derivative glucose oxidase gold osmium derivative polyamine derivative polycation polymer silicon dioxide unclassified drug water adsorption aqueous solution article atomic force microscopy cyclic potentiometry electrochemistry electrode kinetics oxidation reduction reaction protein assembly Biosensing Techniques Electrochemistry Glucose Oxidase Organometallic Compounds Oxidation-Reduction Calvo, E.J. Etchenique, R. Pietrasanta, L. Wolosiuk, A. Danilowicz, C. Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode |
| topic_facet |
Redox polymers Adsorption Agglomeration Cyclic voltammetry Electrochemistry Electrodes Glucose Gold Polymers Quartz Self assembly Enzymes 2,2' diaminoethyldisulfide alkane derivative beta glucose enzyme glucose glucose derivative glucose oxidase gold osmium derivative polyamine derivative polycation polymer silicon dioxide unclassified drug water adsorption aqueous solution article atomic force microscopy cyclic potentiometry electrochemistry electrode kinetics oxidation reduction reaction protein assembly Biosensing Techniques Electrochemistry Glucose Oxidase Organometallic Compounds Oxidation-Reduction |
| description |
The uptake of glucose oxidase (GOx) onto a polycationic redox polymer (PAA-Os)-modified surface, by adsorption from dilute aqueous GOx solutions, was followed by the quartz crystal microbalance (QCM) and shows double exponential kinetics. The electrochemistry of the layer-by-layer-deposited redox-active polymer was followed by cyclic voltammetry in glucose-free solutions, and the enzyme catalysis mediated by the redox polymer was studied in β-D-glucose-containing solutions. AFM studies of the different layers showed the existence of large two dimension enzyme aggregates on the osmium polymer for 1 μM GOx and less aggregation for 50 nM GOx solutions. When the short alkanethiol, 2,2′-diaminoethyldisulfide was preadsorbed onto gold, a monoexponential adsorption law was observed, and single GOx enzyme molecules could be seen on the surface where the enzyme was adsorbed from 50 nM GOx in water. |
| format |
JOUR |
| author |
Calvo, E.J. Etchenique, R. Pietrasanta, L. Wolosiuk, A. Danilowicz, C. |
| author_facet |
Calvo, E.J. Etchenique, R. Pietrasanta, L. Wolosiuk, A. Danilowicz, C. |
| author_sort |
Calvo, E.J. |
| title |
Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode |
| title_short |
Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode |
| title_full |
Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode |
| title_fullStr |
Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode |
| title_full_unstemmed |
Layer-by-layer self-assembly of glucose oxidase and os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode |
| title_sort |
layer-by-layer self-assembly of glucose oxidase and os(bpy)2cipych2nh-poly(allylamine) bioelectrode |
| url |
http://hdl.handle.net/20.500.12110/paper_00032700_v73_n6_p1161_Calvo |
| work_keys_str_mv |
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| _version_ |
1782023674818199552 |