Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points

Methods previously described for glycogen or amylopectin branching enzymatic activity are insufficiently sensitive and not quantitative. A new, more sensitive, specific, and quantitative one was developed. It is based upon the quantitation of the glucose residues joined by α1,6 bonds introduced by v...

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Autores principales: Krisman, C.R., Tolmasky, D.S., Raffo, S.
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00032697_v147_n2_p491_Krisman
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spelling todo:paper_00032697_v147_n2_p491_Krisman2023-10-03T13:55:47Z Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points Krisman, C.R. Tolmasky, D.S. Raffo, S. amylo-α1,4-α1,6-transglucosylase amylopectin branching enzyme assay glycogen quantitation of branching points α1,6-glucosydic linkage 1,4 alpha glucan branching enzyme enzyme assay nonhuman priority journal 1,4-alpha-Glucan Branching Enzyme Animal Glucans Glucosyltransferases Polysaccharides Rabbits Methods previously described for glycogen or amylopectin branching enzymatic activity are insufficiently sensitive and not quantitative. A new, more sensitive, specific, and quantitative one was developed. It is based upon the quantitation of the glucose residues joined by α1,6 bonds introduced by varying amounts of branching enzyme. The procedure involved the synthesis of a polysaccharide from Glc-1-P and phosphorylase in the presence of the sample to be tested. The branched polysaccharide was then purified and the glucoses involved in the branching points were quantitated after degradation with phosphorylase and debranching enzymes. This method appeared to be useful, not only in enzymatic activity determinations but also in the study of the structure of α-d-glucans when combined with those of total polysaccharide quantitation, such as iodine and phenol-sulfuric acid. © 1985. Fil:Krisman, C.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tolmasky, D.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032697_v147_n2_p491_Krisman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic amylo-α1,4-α1,6-transglucosylase
amylopectin
branching enzyme assay
glycogen
quantitation of branching points
α1,6-glucosydic linkage
1,4 alpha glucan branching enzyme
enzyme assay
nonhuman
priority journal
1,4-alpha-Glucan Branching Enzyme
Animal
Glucans
Glucosyltransferases
Polysaccharides
Rabbits
spellingShingle amylo-α1,4-α1,6-transglucosylase
amylopectin
branching enzyme assay
glycogen
quantitation of branching points
α1,6-glucosydic linkage
1,4 alpha glucan branching enzyme
enzyme assay
nonhuman
priority journal
1,4-alpha-Glucan Branching Enzyme
Animal
Glucans
Glucosyltransferases
Polysaccharides
Rabbits
Krisman, C.R.
Tolmasky, D.S.
Raffo, S.
Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
topic_facet amylo-α1,4-α1,6-transglucosylase
amylopectin
branching enzyme assay
glycogen
quantitation of branching points
α1,6-glucosydic linkage
1,4 alpha glucan branching enzyme
enzyme assay
nonhuman
priority journal
1,4-alpha-Glucan Branching Enzyme
Animal
Glucans
Glucosyltransferases
Polysaccharides
Rabbits
description Methods previously described for glycogen or amylopectin branching enzymatic activity are insufficiently sensitive and not quantitative. A new, more sensitive, specific, and quantitative one was developed. It is based upon the quantitation of the glucose residues joined by α1,6 bonds introduced by varying amounts of branching enzyme. The procedure involved the synthesis of a polysaccharide from Glc-1-P and phosphorylase in the presence of the sample to be tested. The branched polysaccharide was then purified and the glucoses involved in the branching points were quantitated after degradation with phosphorylase and debranching enzymes. This method appeared to be useful, not only in enzymatic activity determinations but also in the study of the structure of α-d-glucans when combined with those of total polysaccharide quantitation, such as iodine and phenol-sulfuric acid. © 1985.
format JOUR
author Krisman, C.R.
Tolmasky, D.S.
Raffo, S.
author_facet Krisman, C.R.
Tolmasky, D.S.
Raffo, S.
author_sort Krisman, C.R.
title Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_short Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_full Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_fullStr Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_full_unstemmed Branching enzyme assay: Selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
title_sort branching enzyme assay: selective quantitation of the α1,6-linked glucosyl residues involved in the branching points
url http://hdl.handle.net/20.500.12110/paper_00032697_v147_n2_p491_Krisman
work_keys_str_mv AT krismancr branchingenzymeassayselectivequantitationofthea16linkedglucosylresiduesinvolvedinthebranchingpoints
AT tolmaskyds branchingenzymeassayselectivequantitationofthea16linkedglucosylresiduesinvolvedinthebranchingpoints
AT raffos branchingenzymeassayselectivequantitationofthea16linkedglucosylresiduesinvolvedinthebranchingpoints
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