Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples
A recently introduced redox osmium complex of the type [Os(bpy)2Cl(pyX]+, where pyX corresponds to a pyridine derivative bearing an aldehyde group, and glucose oxidase [EC 1.1.3.4] were covalently immobilized on the glassy carbon electrode surface (upper cell body). This approach has been designed i...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00032670_v443_n1_p17_Lapierre |
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todo:paper_00032670_v443_n1_p17_Lapierre2023-10-03T13:55:43Z Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples Lapierre, A.V. Battaglini, F. Raba, J. Bioreactor Biosensors Flow system Osmium aldehyde blood extract carbon dianisidine functional group glucose glucose oxidase osmium derivative polymer pyridine derivative amperometry article biocatalyst bioreactor blood sampling carbohydrate analysis cell volume comparative study electrode electron transport flow measurement glucose assay glucose blood level Michaelis constant oxidation oxidation reduction reaction pH priority journal reaction time validation process A recently introduced redox osmium complex of the type [Os(bpy)2Cl(pyX]+, where pyX corresponds to a pyridine derivative bearing an aldehyde group, and glucose oxidase [EC 1.1.3.4] were covalently immobilized on the glassy carbon electrode surface (upper cell body). This approach has been designed in a sealed cell to be used under continuous-flow/stopped-flow/continuous-flow processing. The operating characteristic of the integrated bioreactor/detector unit has been utilized in the determination of glucose in serum samples using the electroactive polymer as mediator in the re-oxidation of GOx and amperometric monitoring. The determinations are based on the rate of response under stopped-flow conditions. The trend in values of the apparent Michaelis-Menten constant confirms the validity of the approach to sensitive determination utilizing very small amounts of biocatalysts. The determination of glucose was possible with a limit of detection of 0.020 mM in the processing of as many as 30 samples per hour. © 2001 Elsevier Science B.V. All rights reserved. Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00032670_v443_n1_p17_Lapierre |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bioreactor Biosensors Flow system Osmium aldehyde blood extract carbon dianisidine functional group glucose glucose oxidase osmium derivative polymer pyridine derivative amperometry article biocatalyst bioreactor blood sampling carbohydrate analysis cell volume comparative study electrode electron transport flow measurement glucose assay glucose blood level Michaelis constant oxidation oxidation reduction reaction pH priority journal reaction time validation process |
| spellingShingle |
Bioreactor Biosensors Flow system Osmium aldehyde blood extract carbon dianisidine functional group glucose glucose oxidase osmium derivative polymer pyridine derivative amperometry article biocatalyst bioreactor blood sampling carbohydrate analysis cell volume comparative study electrode electron transport flow measurement glucose assay glucose blood level Michaelis constant oxidation oxidation reduction reaction pH priority journal reaction time validation process Lapierre, A.V. Battaglini, F. Raba, J. Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples |
| topic_facet |
Bioreactor Biosensors Flow system Osmium aldehyde blood extract carbon dianisidine functional group glucose glucose oxidase osmium derivative polymer pyridine derivative amperometry article biocatalyst bioreactor blood sampling carbohydrate analysis cell volume comparative study electrode electron transport flow measurement glucose assay glucose blood level Michaelis constant oxidation oxidation reduction reaction pH priority journal reaction time validation process |
| description |
A recently introduced redox osmium complex of the type [Os(bpy)2Cl(pyX]+, where pyX corresponds to a pyridine derivative bearing an aldehyde group, and glucose oxidase [EC 1.1.3.4] were covalently immobilized on the glassy carbon electrode surface (upper cell body). This approach has been designed in a sealed cell to be used under continuous-flow/stopped-flow/continuous-flow processing. The operating characteristic of the integrated bioreactor/detector unit has been utilized in the determination of glucose in serum samples using the electroactive polymer as mediator in the re-oxidation of GOx and amperometric monitoring. The determinations are based on the rate of response under stopped-flow conditions. The trend in values of the apparent Michaelis-Menten constant confirms the validity of the approach to sensitive determination utilizing very small amounts of biocatalysts. The determination of glucose was possible with a limit of detection of 0.020 mM in the processing of as many as 30 samples per hour. © 2001 Elsevier Science B.V. All rights reserved. |
| format |
JOUR |
| author |
Lapierre, A.V. Battaglini, F. Raba, J. |
| author_facet |
Lapierre, A.V. Battaglini, F. Raba, J. |
| author_sort |
Lapierre, A.V. |
| title |
Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples |
| title_short |
Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples |
| title_full |
Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples |
| title_fullStr |
Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples |
| title_full_unstemmed |
Rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: Application to the determination of glucose in serum samples |
| title_sort |
rotating bioreactor based on an electron transfer mediated by osmium complexes incorporating a continuous-flow/stopped-flow system: application to the determination of glucose in serum samples |
| url |
http://hdl.handle.net/20.500.12110/paper_00032670_v443_n1_p17_Lapierre |
| work_keys_str_mv |
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