Mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level

Single-molecule (SM) fluorescence microscopy was used to investigate the photochromic fluorescent system spiropyran-merocyanine (SP ↔ MC) interacting with gold nanoparticles (AuNPs). We observe a significant increase in the brightness of the emissive MC form, in the duration of its ON time, and in t...

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Autores principales:	Simoncelli, S., Roberti, M.J., Araoz, B., Bossi, M.L., Aramendía, P.F.
Formato:	JOUR
Materias:	Fluorescence Fluorescence microscopy Gold Metal nanoparticles Molecules Emitted photons Fluorescent systems Gold nanoparticle Gold Nanoparticles Photochromic systems Photophysical performance Single-molecule Time monitoring Photochromism gold nanoparticle merocyanine benzopyran derivative fluorescent dye gold indole derivative metal nanoparticle nitro derivative spiropyran accuracy article bleaching brightness controlled study fluorescence microscopy molecular interaction photochemistry photon thermostability chemistry fluorescence Benzopyrans Fluorescent Dyes Indoles Metal Nanoparticles Microscopy, Fluorescence Nitro Compounds
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00027863_v136_n19_p6878_Simoncelli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00027863_v136_n19_p6878_Simoncelli
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spelling	todo:paper_00027863_v136_n19_p6878_Simoncelli2023-10-03T13:54:24Z Mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level Simoncelli, S. Roberti, M.J. Araoz, B. Bossi, M.L. Aramendía, P.F. Fluorescence Fluorescence microscopy Gold Metal nanoparticles Molecules Emitted photons Fluorescent systems Gold nanoparticle Gold Nanoparticles Photochromic systems Photophysical performance Single-molecule Time monitoring Photochromism gold nanoparticle merocyanine benzopyran derivative fluorescent dye gold indole derivative metal nanoparticle nitro derivative spiropyran accuracy article bleaching brightness controlled study fluorescence microscopy molecular interaction photochemistry photon thermostability chemistry fluorescence fluorescence microscopy Benzopyrans Fluorescence Fluorescent Dyes Gold Indoles Metal Nanoparticles Microscopy, Fluorescence Nitro Compounds Single-molecule (SM) fluorescence microscopy was used to investigate the photochromic fluorescent system spiropyran-merocyanine (SP ↔ MC) interacting with gold nanoparticles (AuNPs). We observe a significant increase in the brightness of the emissive MC form, in the duration of its ON time, and in the total number of emitted photons. The spatial distribution of SMs with improved photophysical performance was obtained with 40 nm precision relative to the nearest AuNP. We demonstrate that even photochromic systems with poor photochemical performance for SM can become suitable for long time monitoring and high performance microscopy by interaction with metallic NP. © 2014 American Chemical Society. Fil:Simoncelli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Roberti, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Araoz, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bossi, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aramendía, P.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00027863_v136_n19_p6878_Simoncelli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Fluorescence Fluorescence microscopy Gold Metal nanoparticles Molecules Emitted photons Fluorescent systems Gold nanoparticle Gold Nanoparticles Photochromic systems Photophysical performance Single-molecule Time monitoring Photochromism gold nanoparticle merocyanine benzopyran derivative fluorescent dye gold indole derivative metal nanoparticle nitro derivative spiropyran accuracy article bleaching brightness controlled study fluorescence microscopy molecular interaction photochemistry photon thermostability chemistry fluorescence fluorescence microscopy Benzopyrans Fluorescence Fluorescent Dyes Gold Indoles Metal Nanoparticles Microscopy, Fluorescence Nitro Compounds
spellingShingle	Fluorescence Fluorescence microscopy Gold Metal nanoparticles Molecules Emitted photons Fluorescent systems Gold nanoparticle Gold Nanoparticles Photochromic systems Photophysical performance Single-molecule Time monitoring Photochromism gold nanoparticle merocyanine benzopyran derivative fluorescent dye gold indole derivative metal nanoparticle nitro derivative spiropyran accuracy article bleaching brightness controlled study fluorescence microscopy molecular interaction photochemistry photon thermostability chemistry fluorescence fluorescence microscopy Benzopyrans Fluorescence Fluorescent Dyes Gold Indoles Metal Nanoparticles Microscopy, Fluorescence Nitro Compounds Simoncelli, S. Roberti, M.J. Araoz, B. Bossi, M.L. Aramendía, P.F. Mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level
topic_facet	Fluorescence Fluorescence microscopy Gold Metal nanoparticles Molecules Emitted photons Fluorescent systems Gold nanoparticle Gold Nanoparticles Photochromic systems Photophysical performance Single-molecule Time monitoring Photochromism gold nanoparticle merocyanine benzopyran derivative fluorescent dye gold indole derivative metal nanoparticle nitro derivative spiropyran accuracy article bleaching brightness controlled study fluorescence microscopy molecular interaction photochemistry photon thermostability chemistry fluorescence fluorescence microscopy Benzopyrans Fluorescence Fluorescent Dyes Gold Indoles Metal Nanoparticles Microscopy, Fluorescence Nitro Compounds
description	Single-molecule (SM) fluorescence microscopy was used to investigate the photochromic fluorescent system spiropyran-merocyanine (SP ↔ MC) interacting with gold nanoparticles (AuNPs). We observe a significant increase in the brightness of the emissive MC form, in the duration of its ON time, and in the total number of emitted photons. The spatial distribution of SMs with improved photophysical performance was obtained with 40 nm precision relative to the nearest AuNP. We demonstrate that even photochromic systems with poor photochemical performance for SM can become suitable for long time monitoring and high performance microscopy by interaction with metallic NP. © 2014 American Chemical Society.
format	JOUR
author	Simoncelli, S. Roberti, M.J. Araoz, B. Bossi, M.L. Aramendía, P.F.
author_facet	Simoncelli, S. Roberti, M.J. Araoz, B. Bossi, M.L. Aramendía, P.F.
author_sort	Simoncelli, S.
title	Mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level
title_short	Mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level
title_full	Mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level
title_fullStr	Mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level
title_full_unstemmed	Mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level
title_sort	mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level
url	http://hdl.handle.net/20.500.12110/paper_00027863_v136_n19_p6878_Simoncelli
work_keys_str_mv	AT simoncellis mappingthefluorescenceperformanceofaphotochromicfluorescentsystemcoupledwithgoldnanoparticlesatthesinglemoleculesingleparticlelevel AT robertimj mappingthefluorescenceperformanceofaphotochromicfluorescentsystemcoupledwithgoldnanoparticlesatthesinglemoleculesingleparticlelevel AT araozb mappingthefluorescenceperformanceofaphotochromicfluorescentsystemcoupledwithgoldnanoparticlesatthesinglemoleculesingleparticlelevel AT bossiml mappingthefluorescenceperformanceofaphotochromicfluorescentsystemcoupledwithgoldnanoparticlesatthesinglemoleculesingleparticlelevel AT aramendiapf mappingthefluorescenceperformanceofaphotochromicfluorescentsystemcoupledwithgoldnanoparticlesatthesinglemoleculesingleparticlelevel
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Mapping the fluorescence performance of a photochromic-fluorescent system coupled with gold nanoparticles at the single-molecule-single-particle level

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