Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation

We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregatio...

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Autores principales:	Yushchenko, D.A., Fauerbach, J.A., Thirunavukkuarasu, S., Jares-Erijman, E.A., Jovin, T.M.
Formato:	JOUR
Materias:	Continuous monitoring Dual emissions Fluorescent probes Intermediate stage Parkinsons disease Protein aggregation Ratiometric Sensor molecules Synuclein Probes Fluorescence acetylcysteine alanine alpha synuclein amyloid chromone derivative cysteine maleimide thiol article fluorescence spectroscopy molecular sensor protein aggregation proton transport synthesis alpha-Synuclein Fluorescent Dyes Maleimides Models, Molecular Protein Multimerization Protein Structure, Quaternary Spectrometry, Fluorescence Sulfhydryl Compounds Time Factors
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00027863_v132_n23_p7860_Yushchenko
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00027863_v132_n23_p7860_Yushchenko
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spelling	todo:paper_00027863_v132_n23_p7860_Yushchenko2023-10-03T13:54:13Z Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation Yushchenko, D.A. Fauerbach, J.A. Thirunavukkuarasu, S. Jares-Erijman, E.A. Jovin, T.M. Continuous monitoring Dual emissions Fluorescent probes Intermediate stage Parkinsons disease Protein aggregation Ratiometric Sensor molecules Synuclein Probes Fluorescence acetylcysteine alanine alpha synuclein amyloid chromone derivative cysteine maleimide thiol article fluorescence spectroscopy molecular sensor protein aggregation proton transport synthesis alpha-Synuclein Fluorescent Dyes Maleimides Models, Molecular Protein Multimerization Protein Structure, Quaternary Spectrometry, Fluorescence Sulfhydryl Compounds Time Factors We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease. © 2010 American Chemical Society. Fil:Fauerbach, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00027863_v132_n23_p7860_Yushchenko
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Continuous monitoring Dual emissions Fluorescent probes Intermediate stage Parkinsons disease Protein aggregation Ratiometric Sensor molecules Synuclein Probes Fluorescence acetylcysteine alanine alpha synuclein amyloid chromone derivative cysteine maleimide thiol article fluorescence spectroscopy molecular sensor protein aggregation proton transport synthesis alpha-Synuclein Fluorescent Dyes Maleimides Models, Molecular Protein Multimerization Protein Structure, Quaternary Spectrometry, Fluorescence Sulfhydryl Compounds Time Factors
spellingShingle	Continuous monitoring Dual emissions Fluorescent probes Intermediate stage Parkinsons disease Protein aggregation Ratiometric Sensor molecules Synuclein Probes Fluorescence acetylcysteine alanine alpha synuclein amyloid chromone derivative cysteine maleimide thiol article fluorescence spectroscopy molecular sensor protein aggregation proton transport synthesis alpha-Synuclein Fluorescent Dyes Maleimides Models, Molecular Protein Multimerization Protein Structure, Quaternary Spectrometry, Fluorescence Sulfhydryl Compounds Time Factors Yushchenko, D.A. Fauerbach, J.A. Thirunavukkuarasu, S. Jares-Erijman, E.A. Jovin, T.M. Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
topic_facet	Continuous monitoring Dual emissions Fluorescent probes Intermediate stage Parkinsons disease Protein aggregation Ratiometric Sensor molecules Synuclein Probes Fluorescence acetylcysteine alanine alpha synuclein amyloid chromone derivative cysteine maleimide thiol article fluorescence spectroscopy molecular sensor protein aggregation proton transport synthesis alpha-Synuclein Fluorescent Dyes Maleimides Models, Molecular Protein Multimerization Protein Structure, Quaternary Spectrometry, Fluorescence Sulfhydryl Compounds Time Factors
description	We introduce a sensor molecule, AS140-MFC, consisting of a covalent adduct of an Ala-to-Cys mutant of α-synuclein with the 3-hydroxychromone dual emission dye MFC. We show that the AS140-MFC construct is a multiparametric fluorescent probe suitable for the continuous monitoring of protein aggregation and is sensitive to the early and intermediate stages of α-synuclein aggregation, a process associated with Parkinsons disease. © 2010 American Chemical Society.
format	JOUR
author	Yushchenko, D.A. Fauerbach, J.A. Thirunavukkuarasu, S. Jares-Erijman, E.A. Jovin, T.M.
author_facet	Yushchenko, D.A. Fauerbach, J.A. Thirunavukkuarasu, S. Jares-Erijman, E.A. Jovin, T.M.
author_sort	Yushchenko, D.A.
title	Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title_short	Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title_full	Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title_fullStr	Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title_full_unstemmed	Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation
title_sort	fluorescent ratiometric mfc probe sensitive to early stages of α-synuclein aggregation
url	http://hdl.handle.net/20.500.12110/paper_00027863_v132_n23_p7860_Yushchenko
work_keys_str_mv	AT yushchenkoda fluorescentratiometricmfcprobesensitivetoearlystagesofasynucleinaggregation AT fauerbachja fluorescentratiometricmfcprobesensitivetoearlystagesofasynucleinaggregation AT thirunavukkuarasus fluorescentratiometricmfcprobesensitivetoearlystagesofasynucleinaggregation AT jareserijmanea fluorescentratiometricmfcprobesensitivetoearlystagesofasynucleinaggregation AT jovintm fluorescentratiometricmfcprobesensitivetoearlystagesofasynucleinaggregation
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Fluorescent ratiometric MFC probe sensitive to early stages of α-Synuclein aggregation

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