Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells
Quantum dots multifunctionalized with the amyloid protein α-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's dis...
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| Autores principales: | , , , , , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00027863_v131_n23_p8102_Roberti |
| Aporte de: |
| Sumario: | Quantum dots multifunctionalized with the amyloid protein α-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only ∼100 nanoparticles. The combination with a tetracysteine-tagged form of α-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells. © 2009 American Chemical Society. |
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