Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells
Quantum dots multifunctionalized with the amyloid protein α-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's dis...
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todo:paper_00027863_v131_n23_p8102_Roberti2023-10-03T13:54:10Z Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells Roberti, M.J. Morgan, M. Menéndez, G. Pietrasanta, L.I. Jovin, T.M. Jares-Erijman, E.A. Amyloid formation Amyloid proteins Bulk concentration Fluorogenic In-cell In-vitro Live cell Nanoactuators Nanomolar concentration Parkinson's disease Process diagnostics Quantum Dot Sensitive systems Synuclein Ultrasensitive Glycoproteins Semiconductor quantum dots Cell membranes alpha synuclein amyloid amyloid protein nanoparticle quantum dot fluorescent dye article atomic force microscopy concentration (parameters) female fluorescence microscopy human human cell in vitro study molecular mechanics nanosensor Parkinson disease protein aggregation quantum mechanics chemistry genetic procedures HeLa cell metabolism alpha-Synuclein Amyloid Biosensing Techniques Fluorescent Dyes Hela Cells Humans Microscopy, Atomic Force Microscopy, Fluorescence Quantum Dots Quantum dots multifunctionalized with the amyloid protein α-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only ∼100 nanoparticles. The combination with a tetracysteine-tagged form of α-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells. © 2009 American Chemical Society. Fil:Roberti, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Menéndez, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00027863_v131_n23_p8102_Roberti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amyloid formation Amyloid proteins Bulk concentration Fluorogenic In-cell In-vitro Live cell Nanoactuators Nanomolar concentration Parkinson's disease Process diagnostics Quantum Dot Sensitive systems Synuclein Ultrasensitive Glycoproteins Semiconductor quantum dots Cell membranes alpha synuclein amyloid amyloid protein nanoparticle quantum dot fluorescent dye article atomic force microscopy concentration (parameters) female fluorescence microscopy human human cell in vitro study molecular mechanics nanosensor Parkinson disease protein aggregation quantum mechanics chemistry genetic procedures HeLa cell metabolism alpha-Synuclein Amyloid Biosensing Techniques Fluorescent Dyes Hela Cells Humans Microscopy, Atomic Force Microscopy, Fluorescence Quantum Dots |
| spellingShingle |
Amyloid formation Amyloid proteins Bulk concentration Fluorogenic In-cell In-vitro Live cell Nanoactuators Nanomolar concentration Parkinson's disease Process diagnostics Quantum Dot Sensitive systems Synuclein Ultrasensitive Glycoproteins Semiconductor quantum dots Cell membranes alpha synuclein amyloid amyloid protein nanoparticle quantum dot fluorescent dye article atomic force microscopy concentration (parameters) female fluorescence microscopy human human cell in vitro study molecular mechanics nanosensor Parkinson disease protein aggregation quantum mechanics chemistry genetic procedures HeLa cell metabolism alpha-Synuclein Amyloid Biosensing Techniques Fluorescent Dyes Hela Cells Humans Microscopy, Atomic Force Microscopy, Fluorescence Quantum Dots Roberti, M.J. Morgan, M. Menéndez, G. Pietrasanta, L.I. Jovin, T.M. Jares-Erijman, E.A. Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells |
| topic_facet |
Amyloid formation Amyloid proteins Bulk concentration Fluorogenic In-cell In-vitro Live cell Nanoactuators Nanomolar concentration Parkinson's disease Process diagnostics Quantum Dot Sensitive systems Synuclein Ultrasensitive Glycoproteins Semiconductor quantum dots Cell membranes alpha synuclein amyloid amyloid protein nanoparticle quantum dot fluorescent dye article atomic force microscopy concentration (parameters) female fluorescence microscopy human human cell in vitro study molecular mechanics nanosensor Parkinson disease protein aggregation quantum mechanics chemistry genetic procedures HeLa cell metabolism alpha-Synuclein Amyloid Biosensing Techniques Fluorescent Dyes Hela Cells Humans Microscopy, Atomic Force Microscopy, Fluorescence Quantum Dots |
| description |
Quantum dots multifunctionalized with the amyloid protein α-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only ∼100 nanoparticles. The combination with a tetracysteine-tagged form of α-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells. © 2009 American Chemical Society. |
| format |
JOUR |
| author |
Roberti, M.J. Morgan, M. Menéndez, G. Pietrasanta, L.I. Jovin, T.M. Jares-Erijman, E.A. |
| author_facet |
Roberti, M.J. Morgan, M. Menéndez, G. Pietrasanta, L.I. Jovin, T.M. Jares-Erijman, E.A. |
| author_sort |
Roberti, M.J. |
| title |
Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells |
| title_short |
Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells |
| title_full |
Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells |
| title_fullStr |
Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells |
| title_full_unstemmed |
Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells |
| title_sort |
quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells |
| url |
http://hdl.handle.net/20.500.12110/paper_00027863_v131_n23_p8102_Roberti |
| work_keys_str_mv |
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| _version_ |
1807323336279064576 |