Molecular "wiring" enzymes in organized nanostructures
We report on the "molecular wiring" efficiency of glucose oxidase in organized self-assembled nanostructures comprised of enzyme layers alternating with layers of an osmium-derivatized poly(allylamine) cationic polyelectrolyte, acting as redox relays. Varying the relative position of the a...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00027863_v124_n11_p2452_Calvo |
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todo:paper_00027863_v124_n11_p2452_Calvo2023-10-03T13:53:32Z Molecular "wiring" enzymes in organized nanostructures Calvo, E.J. Danilowicz, C. Wolosiuk, A. apoenzyme avidin biotin enzyme glucose glucose oxidase osmium polyelectrolyte polymer single stranded DNA adsorption amperometry antigen antibody reaction article biosensor cyclic potentiometry diffusion diffusion coefficient DNA hybridization electricity electrochemical analysis electron transport electronics enzyme immobilization enzyme structure glucose oxidation hydrogel isoelectric point Michaelis constant molecular recognition oxidation pH steady state Electrochemistry Glucose Glucose Oxidase Nanotechnology Organometallic Compounds Osmium Oxidation-Reduction Pyridines We report on the "molecular wiring" efficiency of glucose oxidase in organized self-assembled nanostructures comprised of enzyme layers alternating with layers of an osmium-derivatized poly(allylamine) cationic polyelectrolyte, acting as redox relays. Varying the relative position of the active enzyme layer in nanostructures alternating active enzyme and inactive apoenzyme we have demonstrated that the specific rate of bimolecular FADH2 oxidation ("wiring efficiency") is limited by the diffusion-like electron hopping mechanism in the multilayers. Copyright © 2002 American Chemical Society. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00027863_v124_n11_p2452_Calvo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
apoenzyme avidin biotin enzyme glucose glucose oxidase osmium polyelectrolyte polymer single stranded DNA adsorption amperometry antigen antibody reaction article biosensor cyclic potentiometry diffusion diffusion coefficient DNA hybridization electricity electrochemical analysis electron transport electronics enzyme immobilization enzyme structure glucose oxidation hydrogel isoelectric point Michaelis constant molecular recognition oxidation pH steady state Electrochemistry Glucose Glucose Oxidase Nanotechnology Organometallic Compounds Osmium Oxidation-Reduction Pyridines |
| spellingShingle |
apoenzyme avidin biotin enzyme glucose glucose oxidase osmium polyelectrolyte polymer single stranded DNA adsorption amperometry antigen antibody reaction article biosensor cyclic potentiometry diffusion diffusion coefficient DNA hybridization electricity electrochemical analysis electron transport electronics enzyme immobilization enzyme structure glucose oxidation hydrogel isoelectric point Michaelis constant molecular recognition oxidation pH steady state Electrochemistry Glucose Glucose Oxidase Nanotechnology Organometallic Compounds Osmium Oxidation-Reduction Pyridines Calvo, E.J. Danilowicz, C. Wolosiuk, A. Molecular "wiring" enzymes in organized nanostructures |
| topic_facet |
apoenzyme avidin biotin enzyme glucose glucose oxidase osmium polyelectrolyte polymer single stranded DNA adsorption amperometry antigen antibody reaction article biosensor cyclic potentiometry diffusion diffusion coefficient DNA hybridization electricity electrochemical analysis electron transport electronics enzyme immobilization enzyme structure glucose oxidation hydrogel isoelectric point Michaelis constant molecular recognition oxidation pH steady state Electrochemistry Glucose Glucose Oxidase Nanotechnology Organometallic Compounds Osmium Oxidation-Reduction Pyridines |
| description |
We report on the "molecular wiring" efficiency of glucose oxidase in organized self-assembled nanostructures comprised of enzyme layers alternating with layers of an osmium-derivatized poly(allylamine) cationic polyelectrolyte, acting as redox relays. Varying the relative position of the active enzyme layer in nanostructures alternating active enzyme and inactive apoenzyme we have demonstrated that the specific rate of bimolecular FADH2 oxidation ("wiring efficiency") is limited by the diffusion-like electron hopping mechanism in the multilayers. Copyright © 2002 American Chemical Society. |
| format |
JOUR |
| author |
Calvo, E.J. Danilowicz, C. Wolosiuk, A. |
| author_facet |
Calvo, E.J. Danilowicz, C. Wolosiuk, A. |
| author_sort |
Calvo, E.J. |
| title |
Molecular "wiring" enzymes in organized nanostructures |
| title_short |
Molecular "wiring" enzymes in organized nanostructures |
| title_full |
Molecular "wiring" enzymes in organized nanostructures |
| title_fullStr |
Molecular "wiring" enzymes in organized nanostructures |
| title_full_unstemmed |
Molecular "wiring" enzymes in organized nanostructures |
| title_sort |
molecular "wiring" enzymes in organized nanostructures |
| url |
http://hdl.handle.net/20.500.12110/paper_00027863_v124_n11_p2452_Calvo |
| work_keys_str_mv |
AT calvoej molecularwiringenzymesinorganizednanostructures AT danilowiczc molecularwiringenzymesinorganizednanostructures AT wolosiuka molecularwiringenzymesinorganizednanostructures |
| _version_ |
1807322562081849344 |