In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells

Human cystic echinococcosis is a zoonosis caused by the metacestode stage of the tapeworm Echinococcus granulosus. Although benzimidazole compounds such as albendazole and mebendazole have been the cornerstone of chemotherapy for the disease, there is often no complete recovery after treatment. Henc...

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Autores principales:	Pensel, P.E., Albani, C., Gamboa, G.U., Benoit, J.P., Elissondo, M.C.
Formato:	JOUR
Materias:	5-Fluorouracilo Cyst echinococcosis Echinococcus granulosus Hydatid cysts Paclitaxel fluorouracil paclitaxel anticestodal agent cyst drug echinococcosis efficiency measurement experiment laboratory method larva parasite survival time dependent behavior cell organelle chemotherapy disease treatment incubation inoculation parasite infestation animal cell animal experiment animal model animal tissue antiparasitic activity article cell growth cell survival cell type cestode germinal cell concentration response controlled study drug activity drug efficacy female growth inhibition in vitro study metacestode mouse nonhuman parasite viability protoscolex scanning electron microscopy scolex transmission electron microscopy animal dose response drug effects inbred mouse strain anthelmintic activity Article drug effect parasite survival parasite virulence Animals Anticestodal Agents Dose-Response Relationship, Drug Echinococcosis Female Fluorouracil In Vitro Techniques Larva Mice Mice, Inbred Strains
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0001706X_v140_n_p1_Pensel
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0001706X_v140_n_p1_Pensel
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spelling	todo:paper_0001706X_v140_n_p1_Pensel2023-10-03T13:51:40Z In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells Pensel, P.E. Albani, C. Gamboa, G.U. Benoit, J.P. Elissondo, M.C. 5-Fluorouracilo Cyst echinococcosis Echinococcus granulosus Hydatid cysts Paclitaxel fluorouracil paclitaxel anticestodal agent fluorouracil paclitaxel fluorouracil paclitaxel cyst drug echinococcosis efficiency measurement experiment laboratory method larva parasite survival time dependent behavior cell organelle chemotherapy disease treatment incubation inoculation parasite infestation animal cell animal experiment animal model animal tissue antiparasitic activity article cell growth cell survival cell type cestode germinal cell concentration response controlled study drug activity drug efficacy echinococcosis Echinococcus granulosus female growth inhibition in vitro study metacestode mouse nonhuman parasite viability protoscolex scanning electron microscopy scolex transmission electron microscopy animal dose response drug effects echinococcosis Echinococcus granulosus inbred mouse strain larva anthelmintic activity Article drug effect echinococcosis parasite survival parasite virulence Animals Anticestodal Agents Dose-Response Relationship, Drug Echinococcosis Echinococcus granulosus Female Fluorouracil In Vitro Techniques Larva Mice Mice, Inbred Strains Paclitaxel Echinococcus granulosus Human cystic echinococcosis is a zoonosis caused by the metacestode stage of the tapeworm Echinococcus granulosus. Although benzimidazole compounds such as albendazole and mebendazole have been the cornerstone of chemotherapy for the disease, there is often no complete recovery after treatment. Hence, in searching for novel treatment options, we examined the in vitro efficacies of 5-fluorouracil (5-FU) and paclitaxel (PTX) against E. granulosus germinal cells, protoscoleces and cysts. 5-FU or PTX inhibited the growth of E. granulosus cells in a time dependent manner. Although both treatments had a protoscolicidal effect, 5-FU had a considerably stronger effect than PTX. 5-FU produced a dose- and time-dependent effect, provoking the complete loss of viability after 24 days of incubation. Moreover, cysts did not develop following the inoculation of treated protoscoleces into mice. The loss of viability was slower in PTX treated protoscoleces, reaching to approximately 60% after 30 days. The results of the in vitro treatment with 5-FU and PTX were similar in secondary murine cysts. The employment of SEM and TEM allowed us to examine, at an ultrastructural level, the effects induced by 5-FU and PTX on E. granulosus germinal cells, protoscoleces and murine cysts. In conclusion, the data obtained clearly demonstrated that 5-FU and PTX at clinically achievable concentrations inhibit the survival of larval cells, protoscoleces and metacestodes. In vivo studies to test the antiparasitic activities of 5-FU and PTX are currently being undertaken on the murine model of cystic echinococcosis. © 2014 Elsevier B.V. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0001706X_v140_n_p1_Pensel
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-Fluorouracilo Cyst echinococcosis Echinococcus granulosus Hydatid cysts Paclitaxel fluorouracil paclitaxel anticestodal agent fluorouracil paclitaxel fluorouracil paclitaxel cyst drug echinococcosis efficiency measurement experiment laboratory method larva parasite survival time dependent behavior cell organelle chemotherapy disease treatment incubation inoculation parasite infestation animal cell animal experiment animal model animal tissue antiparasitic activity article cell growth cell survival cell type cestode germinal cell concentration response controlled study drug activity drug efficacy echinococcosis Echinococcus granulosus female growth inhibition in vitro study metacestode mouse nonhuman parasite viability protoscolex scanning electron microscopy scolex transmission electron microscopy animal dose response drug effects echinococcosis Echinococcus granulosus inbred mouse strain larva anthelmintic activity Article drug effect echinococcosis parasite survival parasite virulence Animals Anticestodal Agents Dose-Response Relationship, Drug Echinococcosis Echinococcus granulosus Female Fluorouracil In Vitro Techniques Larva Mice Mice, Inbred Strains Paclitaxel Echinococcus granulosus
spellingShingle	5-Fluorouracilo Cyst echinococcosis Echinococcus granulosus Hydatid cysts Paclitaxel fluorouracil paclitaxel anticestodal agent fluorouracil paclitaxel fluorouracil paclitaxel cyst drug echinococcosis efficiency measurement experiment laboratory method larva parasite survival time dependent behavior cell organelle chemotherapy disease treatment incubation inoculation parasite infestation animal cell animal experiment animal model animal tissue antiparasitic activity article cell growth cell survival cell type cestode germinal cell concentration response controlled study drug activity drug efficacy echinococcosis Echinococcus granulosus female growth inhibition in vitro study metacestode mouse nonhuman parasite viability protoscolex scanning electron microscopy scolex transmission electron microscopy animal dose response drug effects echinococcosis Echinococcus granulosus inbred mouse strain larva anthelmintic activity Article drug effect echinococcosis parasite survival parasite virulence Animals Anticestodal Agents Dose-Response Relationship, Drug Echinococcosis Echinococcus granulosus Female Fluorouracil In Vitro Techniques Larva Mice Mice, Inbred Strains Paclitaxel Echinococcus granulosus Pensel, P.E. Albani, C. Gamboa, G.U. Benoit, J.P. Elissondo, M.C. In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells
topic_facet	5-Fluorouracilo Cyst echinococcosis Echinococcus granulosus Hydatid cysts Paclitaxel fluorouracil paclitaxel anticestodal agent fluorouracil paclitaxel fluorouracil paclitaxel cyst drug echinococcosis efficiency measurement experiment laboratory method larva parasite survival time dependent behavior cell organelle chemotherapy disease treatment incubation inoculation parasite infestation animal cell animal experiment animal model animal tissue antiparasitic activity article cell growth cell survival cell type cestode germinal cell concentration response controlled study drug activity drug efficacy echinococcosis Echinococcus granulosus female growth inhibition in vitro study metacestode mouse nonhuman parasite viability protoscolex scanning electron microscopy scolex transmission electron microscopy animal dose response drug effects echinococcosis Echinococcus granulosus inbred mouse strain larva anthelmintic activity Article drug effect echinococcosis parasite survival parasite virulence Animals Anticestodal Agents Dose-Response Relationship, Drug Echinococcosis Echinococcus granulosus Female Fluorouracil In Vitro Techniques Larva Mice Mice, Inbred Strains Paclitaxel Echinococcus granulosus
description	Human cystic echinococcosis is a zoonosis caused by the metacestode stage of the tapeworm Echinococcus granulosus. Although benzimidazole compounds such as albendazole and mebendazole have been the cornerstone of chemotherapy for the disease, there is often no complete recovery after treatment. Hence, in searching for novel treatment options, we examined the in vitro efficacies of 5-fluorouracil (5-FU) and paclitaxel (PTX) against E. granulosus germinal cells, protoscoleces and cysts. 5-FU or PTX inhibited the growth of E. granulosus cells in a time dependent manner. Although both treatments had a protoscolicidal effect, 5-FU had a considerably stronger effect than PTX. 5-FU produced a dose- and time-dependent effect, provoking the complete loss of viability after 24 days of incubation. Moreover, cysts did not develop following the inoculation of treated protoscoleces into mice. The loss of viability was slower in PTX treated protoscoleces, reaching to approximately 60% after 30 days. The results of the in vitro treatment with 5-FU and PTX were similar in secondary murine cysts. The employment of SEM and TEM allowed us to examine, at an ultrastructural level, the effects induced by 5-FU and PTX on E. granulosus germinal cells, protoscoleces and murine cysts. In conclusion, the data obtained clearly demonstrated that 5-FU and PTX at clinically achievable concentrations inhibit the survival of larval cells, protoscoleces and metacestodes. In vivo studies to test the antiparasitic activities of 5-FU and PTX are currently being undertaken on the murine model of cystic echinococcosis. © 2014 Elsevier B.V.
format	JOUR
author	Pensel, P.E. Albani, C. Gamboa, G.U. Benoit, J.P. Elissondo, M.C.
author_facet	Pensel, P.E. Albani, C. Gamboa, G.U. Benoit, J.P. Elissondo, M.C.
author_sort	Pensel, P.E.
title	In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells
title_short	In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells
title_full	In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells
title_fullStr	In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells
title_full_unstemmed	In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells
title_sort	in vitro effect of 5-fluorouracil and paclitaxel on echinococcus granulosus larvae and cells
url	http://hdl.handle.net/20.500.12110/paper_0001706X_v140_n_p1_Pensel
work_keys_str_mv	AT penselpe invitroeffectof5fluorouracilandpaclitaxelonechinococcusgranulosuslarvaeandcells AT albanic invitroeffectof5fluorouracilandpaclitaxelonechinococcusgranulosuslarvaeandcells AT gamboagu invitroeffectof5fluorouracilandpaclitaxelonechinococcusgranulosuslarvaeandcells AT benoitjp invitroeffectof5fluorouracilandpaclitaxelonechinococcusgranulosuslarvaeandcells AT elissondomc invitroeffectof5fluorouracilandpaclitaxelonechinococcusgranulosuslarvaeandcells
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In vitro effect of 5-fluorouracil and paclitaxel on Echinococcus granulosus larvae and cells

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