The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease

Studies were carried out to elucidate if in the hexachlorobenzene (HCB) pophyria, total, nonhaem and haem iron contents in liver are altered and if any relation exists between these alterations and the hepatic porphyrinogen carboxy-lyase (PCL) decrease in rats treated with the drug. It was observed...

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Autores principales: Wainstok De Calmanovici, R., Rios De Molina del, M.C., San Martin De Viale, L.C.
Formato: JOUR
Materias:
ferritin
heme
hexachlorobenzene
iron
uroporphyrinogen decarboxylase
animal experiment
blood and hemopoietic system
intoxication
liver
nonhuman
porphyria
rat
Animal
Carboxy-Lyases
Chlorobenzenes
Disease Models, Animal
Female
Heme
Hexachlorobenzene
Iron
Liver
Porphyria
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Uroporphyrinogen Decarboxylase
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00016764_v35_n4_p481_WainstokDeCalmanovici
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00016764_v35_n4_p481_WainstokDeCalmanovici
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spelling todo:paper_00016764_v35_n4_p481_WainstokDeCalmanovici2023-10-03T13:51:26Z The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease Wainstok De Calmanovici, R. Rios De Molina del, M.C. San Martin De Viale, L.C. ferritin heme hexachlorobenzene iron uroporphyrinogen decarboxylase animal experiment blood and hemopoietic system intoxication liver nonhuman porphyria rat Animal Carboxy-Lyases Chlorobenzenes Disease Models, Animal Female Heme Hexachlorobenzene Iron Liver Porphyria Rats Rats, Inbred Strains Support, Non-U.S. Gov't Uroporphyrinogen Decarboxylase Studies were carried out to elucidate if in the hexachlorobenzene (HCB) pophyria, total, nonhaem and haem iron contents in liver are altered and if any relation exists between these alterations and the hepatic porphyrinogen carboxy-lyase (PCL) decrease in rats treated with the drug. It was observed that in porphyric livers total and non-haem iron levels increased significantly as a consequence of HCB intoxication while this treatment produced a non significant decrease in the haem iron content. Enzymic preparations of porphyric livers filtered through Sephadex G-25 columns which separate the free iron and that has a content of iron-protein greater than those in normals, exhibited a strong inhibition of PCL. Chelating agents, α α' bipyridyl and 8-hydroxyquinoline do not revert such inhibition. The effect in vitro of ferritin, haemin and inorganic iron at different concentrations of normal PCL activity was also assayed. So, it could be observed that inorganic iron and haemin produce slight inhibition of PCL when added in concentrations higher than those corresponding to a porphyric liver (0.08mM and 10-6 M, respectively, as mean in the incubation media). So, they have no physiological significance. Ferritin does not modify the decarboxylation process. From these results it arises that iron does not play a direct role in the decrease of PCL activity in the experimental porphyria by HCB, not being the inhibitor made evident by heating assays. Iron could perhaps stimulate the metabolization of HCB, giving rise to active metabolite. Fil:Wainstok De Calmanovici, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rios De Molina del, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:San Martin De Viale, L.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00016764_v35_n4_p481_WainstokDeCalmanovici
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic ferritin
heme
hexachlorobenzene
iron
uroporphyrinogen decarboxylase
animal experiment
blood and hemopoietic system
intoxication
liver
nonhuman
porphyria
rat
Animal
Carboxy-Lyases
Chlorobenzenes
Disease Models, Animal
Female
Heme
Hexachlorobenzene
Iron
Liver
Porphyria
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Uroporphyrinogen Decarboxylase
spellingShingle ferritin
heme
hexachlorobenzene
iron
uroporphyrinogen decarboxylase
animal experiment
blood and hemopoietic system
intoxication
liver
nonhuman
porphyria
rat
Animal
Carboxy-Lyases
Chlorobenzenes
Disease Models, Animal
Female
Heme
Hexachlorobenzene
Iron
Liver
Porphyria
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Uroporphyrinogen Decarboxylase
Wainstok De Calmanovici, R.
Rios De Molina del, M.C.
San Martin De Viale, L.C.
The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease
topic_facet ferritin
heme
hexachlorobenzene
iron
uroporphyrinogen decarboxylase
animal experiment
blood and hemopoietic system
intoxication
liver
nonhuman
porphyria
rat
Animal
Carboxy-Lyases
Chlorobenzenes
Disease Models, Animal
Female
Heme
Hexachlorobenzene
Iron
Liver
Porphyria
Rats
Rats, Inbred Strains
Support, Non-U.S. Gov't
Uroporphyrinogen Decarboxylase
description Studies were carried out to elucidate if in the hexachlorobenzene (HCB) pophyria, total, nonhaem and haem iron contents in liver are altered and if any relation exists between these alterations and the hepatic porphyrinogen carboxy-lyase (PCL) decrease in rats treated with the drug. It was observed that in porphyric livers total and non-haem iron levels increased significantly as a consequence of HCB intoxication while this treatment produced a non significant decrease in the haem iron content. Enzymic preparations of porphyric livers filtered through Sephadex G-25 columns which separate the free iron and that has a content of iron-protein greater than those in normals, exhibited a strong inhibition of PCL. Chelating agents, α α' bipyridyl and 8-hydroxyquinoline do not revert such inhibition. The effect in vitro of ferritin, haemin and inorganic iron at different concentrations of normal PCL activity was also assayed. So, it could be observed that inorganic iron and haemin produce slight inhibition of PCL when added in concentrations higher than those corresponding to a porphyric liver (0.08mM and 10-6 M, respectively, as mean in the incubation media). So, they have no physiological significance. Ferritin does not modify the decarboxylation process. From these results it arises that iron does not play a direct role in the decrease of PCL activity in the experimental porphyria by HCB, not being the inhibitor made evident by heating assays. Iron could perhaps stimulate the metabolization of HCB, giving rise to active metabolite.
format JOUR
author Wainstok De Calmanovici, R.
Rios De Molina del, M.C.
San Martin De Viale, L.C.
author_facet Wainstok De Calmanovici, R.
Rios De Molina del, M.C.
San Martin De Viale, L.C.
author_sort Wainstok De Calmanovici, R.
title The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease
title_short The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease
title_full The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease
title_fullStr The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease
title_full_unstemmed The role of iron in the hexachlorobenzene induced porphyria. I. Studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease
title_sort role of iron in the hexachlorobenzene induced porphyria. i. studies on different types of iron and its relation with porphyrinogen carboxy-lyase decrease
url http://hdl.handle.net/20.500.12110/paper_00016764_v35_n4_p481_WainstokDeCalmanovici
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