Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern

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Fil: Gambini, Andrés. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Producción Equina. Buenos Aires, Argentina. - CONICET. Buenos Aires, Argentina.

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Autores principales: Gambini, Andrés, Duque Rodríguez, Matteo, Rodríguez, María Belén, Briski, Olinda, Flores Bragulat, Ana P., Demergassi, Natalia, Losinno, Luis, Salamone, Daniel Felipe
Formato: article Artículo publishedVersion
Lenguaje:Inglés
Publicado: 2020
Materias:
HORSE
GENETICS
OOCYTES
IMPLANTATION
ZEBRA
Acceso en línea:http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020gambini
Aporte de:
FAUBA Digital - Facultad de Agronomía (UBA) de Universidad de Buenos Aires
id snrd:2020gambini
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spelling snrd:2020gambini2023-02-10T10:19:05Z Gambini, Andrés Duque Rodríguez, Matteo Rodríguez, María Belén Briski, Olinda Flores Bragulat, Ana P. Demergassi, Natalia Losinno, Luis Salamone, Daniel Felipe 2020 Fil: Gambini, Andrés. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Producción Equina. Buenos Aires, Argentina. - CONICET. Buenos Aires, Argentina. Fil: Duque Rodríguez, Matteo. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina. Fil: Rodríguez, María Belén. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina. Fil: Briski, Olinda. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina. Fil: Flores Bragulat, Ana P. CONICET. Buenos Aires, Argentina. - Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Producción Equina. Río IV, Córdoba, Argentina. Fil: Demergassi, Natalia. Fundación Temaikèn. Belén de Escobar, Buenos Aires, Argentina. Fil: Losinno, Luis. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Producción Equina. Río IV, Córdoba, Argentina. Fil: Salamone, Daniel Felipe. CONICET. Buenos Aires, Argentina. - Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Fisiología Animal. Buenos Aires, Argentina. Several equids have gone extinct and many extant equids are currently considered vulnerable to critically endangered. This work aimed to evaluate whether domestic horse oocytes support preimplantation development of zebra embryos obtained by intracytoplasmic sperm injection (ICSI, zebroid) and cloning, and to study the Hippo signaling path way during the lineage specification of trophectoderm cells and inner cell mass cells. We first showed that zebra and horse sperm cells induce porcine oocyte activation and recruit maternal SMARCA4 during pronuclear formation. SMARCA4 recruitment showed to be independent of the genetic background of the injected sperm. No differences were found in blastocyst rate of ICSI hybrid (zebra spermatozoon into horse egg) embryos relative to the homospecific horse control group. Interestingly, zebra cloned blastocyst rate was significantly higher at day 8. Moreover, most ICSI and cloned horse and zebra blastocysts showed a similar expression pattern of SOX2 and nuclear YAP1 with the majority of the nuclei positive for YAP1, and most SOX2+ nuclei negative for YAP1. Here we demonstrated that horse oocytes support zebra preimplantation development of both, ICSI and cloned embryos, without compromising development to blastocyst, blastocyst cell number neither the expression of SOX2 and YAP1. Our results support the use of domestic horse oocytes as a model to study in vitro zebra embryos on behalf of preservation of valuable genetic. grafs., fot. application/pdf doi:10.1371/journal.pone.0238948 issn:1932-6203 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020gambini eng info:eu-repo/semantics/openAccess openAccess Plos One Vol.15, no.9 e0238948 http://www.plos.org/ HORSE GENETICS OOCYTES IMPLANTATION ZEBRA Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern article info:eu-repo/semantics/article info:ar-repo/semantics/artículo publishedVersion info:eu-repo/semantics/publishedVersion
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-140
collection FAUBA Digital - Facultad de Agronomía (UBA)
language Inglés
orig_language_str_mv eng
topic HORSE
GENETICS
OOCYTES
IMPLANTATION
ZEBRA
spellingShingle HORSE
GENETICS
OOCYTES
IMPLANTATION
ZEBRA
Gambini, Andrés
Duque Rodríguez, Matteo
Rodríguez, María Belén
Briski, Olinda
Flores Bragulat, Ana P.
Demergassi, Natalia
Losinno, Luis
Salamone, Daniel Felipe
Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
topic_facet HORSE
GENETICS
OOCYTES
IMPLANTATION
ZEBRA
description Fil: Gambini, Andrés. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Producción Equina. Buenos Aires, Argentina. - CONICET. Buenos Aires, Argentina.
format article
Artículo
Artículo
publishedVersion
publishedVersion
author Gambini, Andrés
Duque Rodríguez, Matteo
Rodríguez, María Belén
Briski, Olinda
Flores Bragulat, Ana P.
Demergassi, Natalia
Losinno, Luis
Salamone, Daniel Felipe
author_facet Gambini, Andrés
Duque Rodríguez, Matteo
Rodríguez, María Belén
Briski, Olinda
Flores Bragulat, Ana P.
Demergassi, Natalia
Losinno, Luis
Salamone, Daniel Felipe
author_sort Gambini, Andrés
title Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title_short Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title_full Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title_fullStr Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title_full_unstemmed Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
title_sort horse ooplasm supports in vitro preimplantation development of zebra icsi and scnt embryos without compromising yap1 and sox2 expression pattern
publishDate 2020
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2020gambini
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